Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/Sample_Info.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/proteinGroups.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_B4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_B3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_B1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_D4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_D3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_D2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_B2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073445/iMKTPC_D1.rawprimaryOK200frohlich@genzentrum.lmu.deThomas FröhlichData-dependent acquisitionMass SpectrometryBottom-up proteomicssenescenceTestisTranslational modelPeritubular cells; senescencePeritubular cellshttps://www.ebi.ac.uk/pride/archive/projects/PXD073445Peritubular Myoid CellCells were lysed in a buffer consisting of 8 mol/L urea (Carl Roth, Karlsruhe, Germany) in 50 mmol/L ammonium bicarbonate (Riedel-de Haën, Seelze, Germany) and sonicated in a Bandelin Sonoplus HD3200 cup resonator (Bandelin, Berlin, Germany). Cysteine residues were reduced for 30 min at 56 °C using 1,4-dithiothreitol at a concentration of 5 mM. Alkylation was performed for 30 min at room temperature by the addition of iodoacetamide to give a concentration of 15 mM, in darkness. Excess iodoacetamide was quenched by 1,4-dithiothreitol addition (final concentration 10 mmol/L) and a 15 min incubation at room temperature. The digestion of proteins was performed in two steps: (i) lysyl-endopeptidase C (Lys C; Fujifilm Wako, Neuss, Germany) 1:100 enzyme/protein ratio, for 3 h 40 min at 37 °C; (ii) the dilution of the samples with 50 mmol/L ammonium bicarbonate to 1 mol/L urea and overnight digestion with modified porcine trypsin (Promega, Madison, WI, USA) in a 1:50 enzyme/protein -ratio at 37 °C. Digestion was stopped by adding of 1 % formic acid. Nano LC-MS/MS analysis was carried out on an Ultimate 3000 RSLC coupled with a Q Exactive HF-X (both: Thermo Scientific, Waltham, MA, USA). Peptides were injected onto a trap column (Acclaim Pepmap™ 100 µm × 2 cm, C18, 5 µm, 100 Å, Thermo Scientific) at a flow rate of 5 μL/min. The LC-separation of peptides was performed on an EASY-spray column (Pepmap™ RSLC C18, 2 µm, 100 Å, 75 µm × 50 cm, Thermo Scientific, Waltham, MA, USA) with the flow rate set to 250 nL/min. A two-step gradient from 3% B (0.1% (v/v) formic acid in acetonitrile) to 25% B in 160 min followed by a ramp to 40% B for 10 min.. Peptides were analyzed in the data-dependent acquisition (DDA) mode with top 15 MS/MS scans selected per cycle.PrideAcquired MS spectra were processed using MaxQuant (1.6.11.0), with the “match between runs feature” and the label-free quantification feature being activated. UniProt was used to obtain all available entries of C. jacchus from both TrEMBL (unreviewed, 58047 entries, retrieval date: 04.2021) and Swiss-Prot (reviewed, 113 entries, retrieval date: 04.2021), and complemented by MaxQuant′s built-in contaminant database.ProteomicsThomas FröhlichGene Center Laboratory for Functional Genome Analysis Feodor-Lynen-Str. 25 D-81377 MünchenPARTIALCallithrix Jacchusfrohlich@genzentrum.lmu.de10.1007/S00418-026-02489-3Ludwig-Maximilians-Universität München Genzentrum Laboratory for Functional Genome Analysis Feodor-Lynen-Str. 25 81377 MunichGermanyfalseInduction of senescence in a translational cell model for testicular cells by Bleomycin treatment.We aimed to establish a translational aging model of testicular peritubular cells (TPCs), which is based on immortalized nonhuman primate (NHP) peritubular cells (iMKTPCs) from Callithrix jacchus. Cellular senescence was robustly induced within 10 days by exposure to 25 µg/ml bleomycin for 24 h.2026-05-132026-01-23PXD073445NEWT:6945NEWT:184922NEWT:3555NEWT:2NEWT:157546NEWT:35554NEWT:38942NEWT:307972NEWT:32046NEWT:544496NEWT:2102NEWT:2042546NEWT:45351NEWT:43179NEWT:4513NEWT:5722NEWT:55153NCBITaxon:10407NEWT:1736309NEWT:309800NEWT:1211601NEWT:876138NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:10036NEWT:36745NEWT:1351NEWT:1438992NEWT:2649997NEWT:272563NEWT:224326NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:3885NEWT:3888NEWT:1589NEWT:135622NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:3641NEWT:383379NEWT:466585NEWT:10029NEWT:913645NEWT:1000589NEWT:85963NEWT:85962NEWT:317447NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:192875NEWT:34305NEWT:59729NCBITaxon:183674NEWT:224308NEWT:84645NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:1283NEWT:931281NEWT:4550NEWT:1000561NEWT:197NEWT:1390363NEWT:288705NCBITaxon:79824NEWT:4787NCBITaxon:4563NEWT:5755NEWT:44689NEWT:3218NEWT:5759NEWT:1736231NEWT:1270NEWT:374990NEWT:2242NEWT:4784NEWT:11320NEWT:360106NEWT:286NEWT:391619NEWT:287NEWT:10117NEWT:10239NEWT:10116NEWT:1280NEWT:1735272NEWT:83334NEWT:83332NEWT:44685NEWT:317513NEWT:1148NEWT:580240NEWT:294128NEWT:11676NEWT:55571NEWT:100226NEWT:4530NEWT:4896NEWT:75058NEWT:13616NEWT:1390NEWT:1094343NEWT:296543NEWT:1773NEWT:1895NEWT:1182590NEWT:3712NEWT:105023NEWT:935293NEWT:64152NEWT:4924NEWT:749200NEWT:375146NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:263NEWT:230741NEWT:52283NEWT:284812NCBITaxon:1313NEWT:43330NEWT:1603293NEWT:408169NEWT:44544NEWT:4911NEWT:645463NEWT:3702NEWT:129249NEWT:243277NEWT:990119NEWT:408172NEWT:408170NEWT:493760NEWT:260710NEWT:257313NEWT:400772NEWT:3708NEWT:128161NEWT:332648NEWT:106592NEWT:536231NEWT:460519NEWT:1187947NEWT:1432138NEWT:10312NEWT:1424507NCBITaxon:1773NEWT:9598NEWT:8030NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:67767NEWT:432359NEWT:46835NEWT:1182263NEWT:2711NEWT:376686NEWT:95486NEWT:9103NEWT:29159NEWT:253NEWT:10306NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:8022NEWT:145943NCBITaxon:4932NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:3635NEWT:5811NEWT:235443NEWT:108458NEWT:272623NEWT:272624NEWT:411483NEWT:884019NEWT:198215NEWT:411490NEWT:983964NEWT:169963NEWT:32644NEWT:225117NEWT:499175NEWT:109779NEWT:476272NEWT:3747NEWT:195051NEWT:367830NEWT:1255228NEWT:178616NEWT:410289NEWT:373153NEWT:352472NEWT:357NEWT:360094NEWT:470NEWT:1313NEWT:411469NEWT:84023NEWT:559292NEWT:39491NCBITaxon:5811NEWT:411464NEWT:411460NEWT:2014887NEWT:2762NEWT:1174673NEWT:562NEWT:411470NEWT:33952NEWT:2094720NCBITaxon:2697049NEWT:571256NEWT:28038NEWT:1663NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:327159NEWT:178876NEWT:327160NEWT:573NEWT:9031NEWT:7091NEWT:108931NEWT:241368NEWT:42528NEWT:190802NEWT:9778NEWT:150475NEWT:303NEWT:9417NEWT:7111NEWT:347515NEWT:1216979NEWT:5180NEWT:256737NEWT:9541NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:185579NEWT:941442NEWT:220668NEWT:13076NEWT:1249668NEWT:7108NEWT:317NEWT:7227NEWT:7469NEWT:885318NEWT:9402NEWT:415540NEWT:550NEWT:675060NEWT:4081NEWT:334542NEWT:554NEWT:98334NEWT:426428NEWT:1276815NEWT:7574NEWT:1715256NEWT:7215NEWT:575412NEWT:29204NEWT:2172103NEWT:507601NEWT:643680NCBITaxon:6157NEWT:746360NEWT:6239NEWT:470150NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:73239NEWT:226186NEWT:1268063NEWT:8782NEWT:1263854NEWT:435590NEWT:1902NEWT:160488NEWT:28104NEWT:1908NEWT:13164NEWT:216129NCBITaxon:2NEWT:985076NEWT:1215323NEWT:52641NEWT:7038NEWT:6192NEWT:28532NCBITaxon:38727NEWT:353152NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:1194669NEWT:243230NEWT:8355NEWT:9685NEWT:7029NEWT:1080772NEWT:8479NEWT:1283300NEWT:6183NEWT:6063NEWT:630NEWT:334747NEWT:61235NEWT:15368NEWT:6289NEWT:436486NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:170187NEWT:469008NEWT:260707NEWT:256318NCBITaxon:6191NEWT:1836NEWT:185431NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:80863NEWT:2697049NEWT:105231NEWT:1216981NEWT:4097NCBITaxon:6073NEWT:884204NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NEWT:607699NEWT:6282NEWT:208964NEWT:1134506NEWT:575584NEWT:38783NEWT:8727NEWT:4006NEWT:8726NEWT:6426NEWT:6669NEWT:10090NEWT:4120NEWT:51515NEWT:5693NEWT:8724NEWT:51511NEWT:92867NEWT:8723NEWT:5334NEWT:37334NEWT:382352NCBITaxon:10359NEWT:242619NEWT:632957NEWT:34073NEWT:373995NEWT:5689NEWT:544404NEWT:9925NEWT:8839NEWT:4232NEWT:2758385NEWT:37349NEWT:4113NEWT:837NEWT:11298NEWT:171101NEWT:714NEWT:36015NEWT:421932NEWT:196627NEWT:5691NEWT:48479NEWT:627025NEWT:1097677NEWT:61674NEWT:1117957NEWT:9913NEWT:8709NEWT:4100NEWT:1076NEWT:6763NEWT:803NEWT:29722NEWT:380394NEWT:1692259NEWT:180066NEWT:817NEWT:135588NEWT:1843183NEWT:58002NEWT:326423NEWT:36111NEWT:4577NEWT:1416333NEWT:5664NEWT:2157NEWT:1678078NEWT:749906NEWT:418985NEWT:146479NEWT:749907NEWT:1911079NEWT:69373NEWT:264203NEWT:1480154NEWT:1274414NEWT:27606NEWT:59202NEWT:9975NEWT:9612NEWT:38865NEWT:51953NEWT:3197NEWT:9615NEWT:10299NEWT:860688NEWT:112273NEWT:36329NEWT:1147787NCBITaxon:3044782NEWT:1440772NEWT:72407NEWT:1355477NEWT:349741NEWT:9605NEWT:9606NEWT:90371NEWT:157295NEWT: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