{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1.pr_matrix.tsv","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1.pg_matrix.tsv"],"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/checksum.txt"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-D1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-CK1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-CK3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-D3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-W1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-W4.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-D2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-D4.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-W3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-CK2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-W2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073447/YAS202407230073-1-CK4.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["a1198494634@163.com"],"submitter":["Chaoran Song"],"technology_type":["Mass Spectrometry","Data-independent acquisition"],"software":["Not available"],"submitter_keywords":["precipitation","proteomics","endogenous hormones","vicia costata","Desert steppe; vicia costata; endogenous hormones; precipitation; proteomics","Desert steppe"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD073447"],"sample_protocol":["An appropriate amount of SDT lysis buffer (4% SDS, 100 mM Tris-HCl, pH 7.6) was added to each sample for protein extraction, and the BCA method was used for protein quantification. 15 μg of protein from each sample was mixed with an appropriate amount of 5X loading buffer, boiled for 5 min, and subjected to SDS-PAGE electrophoresis (4%-20% precast gradient gel, constant voltage 180 V, 45 min), followed by Coomassie Brilliant Blue R-250 staining. An appropriate amount of protein from all samples was mixed to form a Pool sample, which was used as a QC sample.  DIA analysis was performed using a nano-flow Vanquish Neo system (Thermo Fisher) for chromatographic separation. The samples after nano-scale high-performance liquid chromatography separation were analyzed by an Astral high-resolution mass spectrometer (Thermo Scientific) in DIA (Data-Independent Acquisition) mode. Detection mode: positive ion; parent ion scanning range: 380-980 m/z; primary mass spectrometry resolution: 240,000 at 200 m/z; Normalized AGC Target: 500%; Maximum IT: 5 ms. For MS2, the DIA data acquisition mode was set with 299 scanning windows, Isolation Window of 2 m/z, HCD Collision Energy of 25 ev, Normalized AGC Target of 500%, and Maximum IT of 3 ms."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["An appropriate amount of SDT lysis buffer (4% SDS, 100 mM Tris-HCl, pH 7.6) was added to each sample for protein extraction, and the BCA method was used for protein quantification. 15 μg of protein from each sample was mixed with an appropriate amount of 5X loading buffer, boiled for 5 min, and subjected to SDS-PAGE electrophoresis (4%-20% precast gradient gel, constant voltage 180 V, 45 min), followed by Coomassie Brilliant Blue R-250 staining. An appropriate amount of protein from all samples was mixed to form a Pool sample, which was used as a QC sample.  DIA analysis was performed using a nano-flow Vanquish Neo system (Thermo Fisher) for chromatographic separation. The samples after nano-scale high-performance liquid chromatography separation were analyzed by an Astral high-resolution mass spectrometer (Thermo Scientific) in DIA (Data-Independent Acquisition) mode. Detection mode: positive ion; parent ion scanning range: 380-980 m/z; primary mass spectrometry resolution: 240,000 at 200 m/z; Normalized AGC Target: 500%; Maximum IT: 5 ms. For MS2, the DIA data acquisition mode was set with 299 scanning windows, Isolation Window of 2 m/z, HCD Collision Energy of 25 ev, Normalized AGC Target of 500%, and Maximum IT of 3 ms."],"omics_type":["Proteomics"],"labhead":["Song Chaoran"],"instrument_platform":[""],"labhead_affiliation":["Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences"],"submission_type":["PARTIAL"],"species":["Vicia Costata"],"publication":["Not available"],"submitter_mail":["a1198494634@163.com"],"submitter_affiliation":["no"],"submitter_country":["China"],"additional_accession":[]},"is_claimable":false,"name":"Effects of Precipitation Changes on Endogenous Hormones, Metabolomics, and Proteomics of Vicia costata in Desert Steppe","description":"This study systematically investigated the regulatory mechanisms of precipitation changes on endogenous hormones, metabolome, and proteome of Vicia costata Ledeb., a key leguminous plant in desert steppe. Three precipitation treatments were established: 60% precipitation reduction (D), normal precipitation (CK), and 60% precipitation increase (W). Targeted plant hormone metabolomics, untargeted metabolomics, and proteomics technologies were employed to comprehensively analyze the response characteristics. The results showed that precipitation changes significantly affected the balance of endogenous hormones in Vicia costata. Six hormones, including ABA, ACC, cis-OPDA, cZR, tZ, and Typhasterol, exhibited significant differences among different treatments. A total of 1,058 metabolites were identified through metabolomics, with lipids and lipid-like molecules accounting for the highest proportion (30.34%). Differential metabolites were significantly enriched in pathways such as ABC transporters, phenylpropanoid biosynthesis, carbon fixation, and zeatin biosynthesis. Proteomic analysis revealed that the number of differential proteins was the highest in the D vs W comparison (41 proteins), which were significantly enriched in pathways including MAPK signaling pathway, starch and sucrose metabolism, and endoplasmic reticulum protein processing. Integrated analysis identified four common pathways (e.g., plant hormone signal transduction, starch and sucrose metabolism) that synergistically responded to water changes. This study uncovered the molecular adaptation mechanisms of Vicia costata to precipitation changes at the multi-omics level, providing a theoretical basis for the selection, breeding, and utilization of Vicia costata.","dates":{"publication":"2026-05-26","submission":"2026-01-23"},"accession":"PXD073447","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:184922","NEWT:3555","NEWT:2","NEWT:157546","NEWT:35554","NEWT:38942","NEWT:307972","NEWT:32046","NEWT:544496","NEWT:2102","NEWT:2042546","NEWT:45351","NEWT:43179","NEWT:4513","NEWT:5722","NEWT:55153","NCBITaxon:10407","NEWT:1736309","NEWT:309800","NEWT:1211601","NEWT:876138","NEWT:3654","NEWT:237561","NEWT:5833","NEWT:6928","NEWT:10036","NEWT:36745","NEWT:1351","NEWT:1438992","NEWT:2649997","NEWT:272563","NEWT:224326","NCBITaxon:79857","NEWT:1096976","NEWT:95648","NEWT:3885","NEWT:3888","NEWT:1589","NEWT:135622","NCBITaxon:4896","NEWT:6915","NEWT:3649","NEWT:101510","NEWT:28903","NEWT:3880","NEWT:272559","NEWT:3641","NEWT:383379","NEWT:466585","NEWT:10029","NEWT:913645","NEWT:1000589","NEWT:85963","NEWT:85962","NEWT:317447","NEWT:7955","NEWT:7959","NEWT:2261","NEWT:31156","NEWT:398580","NEWT:4565","NEWT:1264690","NEWT:515619","NEWT:192875","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:84645","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:5888","NEWT:1283","NEWT:931281","NEWT:4550","NEWT:1000561","NEWT:197","NEWT:1390363","NEWT:288705","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:44689","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:1270","NEWT:374990","NEWT:2242","NEWT:4784","NEWT:11320","NEWT:360106","NEWT:286","NEWT:391619","NEWT:287","NEWT:10117","NEWT:10239","NEWT:10116","NEWT:1280","NEWT:1735272","NEWT:83334","NEWT:83332","NEWT:44685","NEWT:317513","NEWT:1148","NEWT:580240","NEWT:294128","NEWT:11676","NEWT:55571","NEWT:100226","NEWT:4530","NEWT:4896","NEWT:75058","NEWT:13616","NEWT:1390","NEWT:1094343","NEWT:296543","NEWT:1773","NEWT:1895","NEWT:1182590","NEWT:3712","NEWT:105023","NEWT:935293","NEWT:64152","NEWT:4924","NEWT:749200","NEWT:375146","NEWT:990346","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:263","NEWT:230741","NEWT:52283","NEWT:284812","NCBITaxon:1313","NEWT:43330","NEWT:1603293","NEWT:408169","NEWT:44544","NEWT:4911","NEWT:645463","NEWT:3702","NEWT:129249","NEWT:243277","NEWT:990119","NEWT:408172","NEWT:408170","NEWT:493760","NEWT:260710","NEWT:257313","NEWT:400772","NEWT:3708","NEWT:128161","NEWT:332648","NEWT:106592","NEWT:536231","NEWT:460519","NEWT:1187947","NEWT:1432138","NEWT:10312","NEWT:1424507","NCBITaxon:1773","NEWT:9598","NEWT:8030","NEWT:9483","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:67767","NEWT:432359","NEWT:46835","NEWT:1182263","NEWT:2711","NEWT:376686","NEWT:95486","NEWT:9103","NEWT:29159","NEWT:253","NEWT:10306","NCBITaxon:2759","NEWT:1233435","NEWT:93061","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NEWT:240906","NEWT:593117","NEWT:89920","NEWT:3635","NEWT:5811","NEWT:235443","NEWT:108458","NEWT:272623","NEWT:272624","NEWT:411483","NEWT:884019","NEWT:198215","NEWT:411490","NEWT:983964","NEWT:169963","NEWT:32644","NEWT:225117","NEWT:499175","NEWT:109779","NEWT:476272","NEWT:3747","NEWT:195051","NEWT:367830","NEWT:1255228","NEWT:178616","NEWT:410289","NEWT:373153","NEWT:352472","NEWT:357","NEWT:360094","NEWT:470","NEWT:1313","NEWT:411469","NEWT:84023","NEWT:559292","NEWT:39491","NCBITaxon:5811","NEWT:411464","NEWT:411460","NEWT:2014887","NEWT:2762","NEWT:1174673","NEWT:562","NEWT:411470","NEWT:33952","NEWT:2094720","NCBITaxon:2697049","NEWT:571256","NEWT:28038","NEWT:1663","NEWT:1423","NEWT:4932","NEWT:3603","NEWT:2759","NEWT:3847","NEWT:327159","NEWT:178876","NEWT:327160","NEWT:573","NEWT:9031","NEWT:7091","NEWT:108931","NEWT:241368","NEWT:42528","NEWT:190802","NEWT:9778","NEWT:150475","NEWT:303","NEWT:9417","NEWT:7111","NEWT:347515","NEWT:1216979","NEWT:5180","NEWT:256737","NEWT:9541","NEWT:115104","NEWT:1121114","NEWT:663","NEWT:1081927","NEWT:1238993","NEWT:67825","NEWT:185579","NEWT:941442","NEWT:220668","NEWT:13076","NEWT:1249668","NEWT:7108","NEWT:317","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:9402","NEWT:415540","NEWT:550","NEWT:675060","NEWT:4081","NEWT:334542","NEWT:554","NEWT:98334","NEWT:426428","NEWT:7574","NEWT:1715256","NEWT:7215","NEWT:575412","NEWT:29204","NEWT:2172103","NEWT:7460","NEWT:507601","NEWT:643680","NCBITaxon:6157","NEWT:746360","NEWT:6239","NEWT:470150","NEWT:216257","NEWT:102169","NEWT:9986","NEWT:4054","NEWT:73239","NEWT:226186","NEWT:1268063","NEWT:8782","NEWT:1263854","NEWT:435590","NEWT:1902","NEWT:160488","NEWT:28104","NEWT:1908","NEWT:13164","NEWT:216129","NCBITaxon:2","NEWT:985076","NEWT:1215323","NEWT:52641","NEWT:7038","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:353152","NEWT:2829","NEWT:366581","NEWT:216599","NEWT:216595","NEWT:1194669","NEWT:51329","NEWT:243230","NEWT:8355","NEWT:9685","NEWT:7029","NEWT:1080772","NEWT:8479","NEWT:1283300","NEWT:6183","NEWT:6063","NEWT:630","NEWT:334747","NEWT:61235","NEWT:15368","NEWT:6289","NEWT:436486","NEWT:6287","NEWT:300641","NEWT:727","NEWT:9796","NEWT:725","NEWT:170187","NEWT:469008","NEWT:260707","NEWT:256318","NCBITaxon:6191","NEWT:1836","NEWT:1854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