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The tissue was incubated in 4 mL of wash buffer [1mM DTT PBS with protease inhibitor (Roche, 4693159001)] on ice for 10 min and vortexed for 20 seconds. After the removal of tissue pieces, intestinal washes were centrifuged at 100 xg for 1 min to remove the large pieces of intestinal contents. The supernatant was concentrated by centrifugation at 4000 xg with 3 kDa MWCO Amicon Ultra centrifugal filter unit for 1 hour. The volume of the concentrate was adjusted to 400 mL with wash buffer. The supernatants were processed for LC-MS/MS analysis using the ENRICH-iST kit (PreOmics, #P.O.00163). Assay details have been described previously, including protein enrichment, denaturation, reduction, alkylation, enzymatic digestion, and peptide cleanup within a single workflow. Briefly, mucus proteins were captured on paramagnetic enrichment beads in binding buffer, followed by multiple bead-based wash steps to remove salts, lipids, and low-molecular-weight contaminants. Bead-captured proteins were denatured, reduced, and alkylated using the supplied lysis buffer at elevated temperature and digested on-bead with a Trypsin/LysC protease mixture. Digestion was terminated by stop solution, and peptides were purified using the solid-phase extraction cartridge with sequential washes to remove impurities. Peptides were eluted in volatile buffer, dried completely by vacuum centrifugation (SpeedVac SPD120), and stored at −80 °C until LC-MS/MS analysis.Peptides were reconstituted in 5-10 µL of solvent A (2.5% acetonitrile, 0.1% formic acid) and separated by nano-scale reverse-phase liquid chromatography using a custom-packed capillary column (100 µm inner diameter × ~30 cm length) containing 2.6 µm C18 spherical silica beads and terminated with a flame-drawn electrospray tip. Subsequently, peptides were loaded via an autosampler and eluted using a linear gradient of increasing concentrations of solvent B (97.5% acetonitrile, 0.1% formic acid). Eluting peptides were ionized by electrospray and analyzed on an Orbitrap Velos Pro hybrid ion trap-Orbitrap mass spectrometer (Thermo Fisher Scientific)."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["MS raw files were searched against the UniProt FASTA databases using Sequest (Thermo Fisher Scientific). Databases included reversed (decoy) sequences to enable estimation of false discovery rates. Peptide-spectrum matches were filtered to achieve a peptide-level false discovery rate of 1–2%. Carbamidomethylation of cysteine residues was specified as a fixed modification during database searching. Enzyme specificity was defined as cleavage at the carboxyl terminus of arginine and lysine residues, consistent with digestion by trypsin and LysC. A maximum of 1 missed cleavages was allowed. All bioinformatic analyses were performed using Perseus (version 2.0.7.0) as well as standard analytical workflows implemented in R (version 4.5.2)."],"omics_type":["Proteomics"],"labhead":["Matthew K Waldor"],"instrument_platform":[""],"submission_type":["COMPLETE"],"labhead_affiliation":["1.Division of Infectious Diseases, Brigham and Women's Hospital, Boston, MA, USA 2.Department of Microbiology, Harvard Medical School, Boston, MA, USA 3.Howard Hughes Medical Institute, Boston, MA, USA"],"species":["Mus Musculus (mouse)"],"submitter_mail":["zliang3@bwh.harvard.edu"],"publication":["Not available"],"submitter_affiliation":["Brigham and Women's Hospital, Harvard Medical School"],"submitter_country":["United States"],"doi":["10.6019/PXD075406"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic analysis of small intestine luminal contents in IL22Fc-treated mouse","description":"Proteomic analyis was perfomred to identify the IL22Fc-induced proteins/polypeptides in  the murine small intestine contents","dates":{"publication":"2026-04-13","submission":"2026-03-09"},"accession":"PXD075406","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:3555","NEWT:241368","NEWT:2","NEWT:157546","NEWT:190802","NEWT:35554","NEWT:9778","NEWT:150475","NEWT:9417","NEWT:347515","NEWT:1216979","NEWT:307972","NEWT:32046","NEWT:544496","NEWT:5180","NEWT:256737","NEWT:2042546","NEWT:115104","NEWT:1081927","NEWT:67825","NEWT:43179","NEWT:13076","NEWT:1249668","NEWT:317","NEWT:55153","NEWT:1736309","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:415540","NEWT:4081","NEWT:876138","NEWT:554","NEWT:98334","NEWT:426428","NEWT:237561","NEWT:6928","NEWT:10036","NEWT:7574","NEWT:1351","NEWT:7215","NEWT:29204","NEWT:272563","NEWT:507601","NCBITaxon:79857","NCBITaxon:6157","NEWT:95648","NEWT:3885","NEWT:746360","NEWT:6239","NEWT:1589","NEWT:470150","NEWT:135622","NEWT:216257","NEWT:6915","NEWT:9986","NEWT:101510","NEWT:4054","NEWT:3880","NEWT:3641","NEWT:8782","NEWT:1263854","NEWT:1000589","NEWT:1902","NEWT:85962","NEWT:160488","NEWT:28104","NEWT:317447","NEWT:7955","NCBITaxon:2","NEWT:985076","NEWT:7959","NEWT:2261","NEWT:4565","NEWT:1264690","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:216595","NEWT:243230","NEWT:8355","NEWT:1283","NEWT:931281","NEWT:1000561","NEWT:7029","NEWT:1283300","NEWT:6183","NEWT:334747","NEWT:61235","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:436486","NEWT:6287","NEWT:2242","NEWT:300641","NEWT:4784","NEWT:727","NEWT:9796","NEWT:725","NEWT:360106","NEWT:260707","NEWT:287","NEWT:10117","NEWT:10239","NCBITaxon:6191","NEWT:10116","NEWT:1280","NEWT:1836","NEWT:1735272","NEWT:83334","NEWT:185431","NEWT:83332","NEWT:29760","NEWT:260704","NEWT:703612","NEWT:260705","NEWT:80863","NEWT:44685","NEWT:2697049","NEWT:1148","NEWT:11676","NEWT:55571","NEWT:100226","NCBITaxon:6073","NEWT:4530","NEWT:4896","NEWT:6279","NEWT:1123869","NEWT:7370","NEWT:75058","NEWT:83906","NEWT:607699","NEWT:6282","NEWT:208964","NEWT:1134506","NEWT:575584","NEWT:296543","NEWT:1773","NEWT:38783","NEWT:8727","NEWT:1895","NEWT:1182590","NEWT:8726","NEWT:6669","NEWT:10090","NEWT:935293","NEWT:64152","NEWT:749200","NEWT:4120","NEWT:51515","NEWT:5693","NEWT:8724","NEWT:51511","NEWT:92867","NEWT:8723","NEWT:990346","NEWT:5334","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:230741","NEWT:284812","NCBITaxon:10359","NCBITaxon:1313","NEWT:43330","NEWT:242619","NEWT:44544","NEWT:632957","NEWT:373995","NEWT:645463","NEWT:544404","NEWT:3702","NEWT:129249","NEWT:9925","NEWT:8839","NEWT:4232","NEWT:990119","NEWT:2758385","NEWT:4113","NEWT:837","NEWT:11298","NEWT:171101","NEWT:421932","NEWT:196627","NEWT:408172","NEWT:5691","NEWT:408170","NEWT:493760","NEWT:260710","NEWT:627025","NEWT:400772","NEWT:1097677","NEWT:3708","NEWT:128161","NEWT:106592","NEWT:1117957","NEWT:9913","NEWT:1432138","NEWT:10312","NEWT:1424507","NEWT:4100","NEWT:1076","NEWT:6763","NEWT:803","NEWT:8030","NEWT:29722","NEWT:380394","NEWT:1692259","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:180066","NEWT:67767","NEWT:46835","NEWT:135588","NEWT:1843183","NEWT:95486","NEWT:58002","NEWT:9103","NEWT:4577","NEWT:5664","NEWT:2157","NEWT:146479","NEWT:10306","NEWT:1911079","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NEWT:3635","NEWT:5811","NEWT:1480154","NEWT:235443","NEWT:1274414","NEWT:59202","NEWT:9975","NEWT:3197","NEWT:9615","NEWT:10299","NEWT:860688","NEWT:884019","NEWT:169963","NEWT:36329","NEWT:1147787","NEWT:72407","NEWT:9606","NEWT:367830","NEWT:157295","NEWT:178616","NEWT:410289","NEWT:373153","NEWT:915099","NEWT:74940","NEWT:1450511","NEWT:360094","NEWT:470","NEWT:84023","NEWT:9838","NCBITaxon:9615","NEWT:58334","NEWT:1193501","NEWT:3055","NEWT:6326","NEWT:6689","NEWT:2762","NEWT:5476","NEWT:1174673","NEWT:562","NEWT:33952","NEWT:1274432","NEWT:1274426","NEWT:1423","NEWT:4932","NEWT:70448","NEWT:9825","NEWT:1274423","NEWT:3603","NEWT:698936","NEWT:2759","NEWT:3847","NEWT:39946","NEWT:9823","NEWT:9940","NEWT:327160","NEWT:573","NEWT:9031","NEWT:1274420","NEWT:7091"]}}