Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/SampleLabels.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/250621_Hepatocytes_TimeCourse_Report_Rename_without25.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0621_AS2_001614_MRL_Hep_ARA_10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1014_AS2_001617_MRL_Hep_ARA_13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0503_AS2_001613_MRL_Hep_ARA_9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-2154_AS2_001626_MRL_Hep_ARA_22.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0029_AS2_001628_MRL_Hep_ARA_24.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1247_AS2_001619_MRL_Hep_ARA_15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250617-2115_AS2_001607_MRL_Hep_ARA_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250617-2232_AS2_001608_MRL_Hep_ARA_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0345_AS2_001612_MRL_Hep_ARA_8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0147_AS2_001629_MRL_Hep_ARA_25.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250617-1838_AS2_001605_MRL_Hep_ARA_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250617-2350_AS2_001609_MRL_Hep_ARA_5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-2312_AS2_001627_MRL_Hep_ARA_23.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0108_AS2_001610_MRL_Hep_ARA_6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0739_AS2_001615_MRL_Hep_ARA_11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-2037_AS2_001625_MRL_Hep_ARA_21.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0226_AS2_001611_MRL_Hep_ARA_7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-0858_AS2_001616_MRL_Hep_ARA_12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1524_AS2_001621_MRL_Hep_ARA_17.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250617-1957_AS2_001606_MRL_Hep_ARA_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1642_AS2_001622_MRL_Hep_ARA_18.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1801_AS2_001623_MRL_Hep_ARA_19.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0420_AS2_001631_MRL_Hep_ARA_27.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1406_AS2_001620_MRL_Hep_ARA_16.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1919_AS2_001624_MRL_Hep_ARA_20.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250618-1129_AS2_001618_MRL_Hep_ARA_14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0814_AS2_001634_MRL_Hep_ARA_30.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0656_AS2_001633_MRL_Hep_ARA_29.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0303_AS2_001630_MRL_Hep_ARA_26.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD075440/20250619-0538_AS2_001632_MRL_Hep_ARA_28.rawprimaryOK200grevengoed@sund.ku.dkIvan BradićMass SpectrometryBottom-up proteomicsFatty acid oxidationEndogenous lipid metaboliteArachidonic acidN-acyl taurineMasldAra-thttps://www.ebi.ac.uk/pride/archive/projects/PXD075440HepatocyteHepatocytes were lysed in 1% sodium deoxycholate (SDC) in 50 mM Hepes (pH 8.5) containing protease (Roche Complete protease inhibitor EDTA free 118735800001) and phosphatase (PhosphoStop 04906837001, Sigma) inhibitors. Afterward, samples were sonicated twice for 30 s at 60% amplitude, proteins were denatured at 95°C for 5 min, and centrifuged for 30 min at 20,000 x g. Protein concentration was determined in supernatant using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Twenty micrograms of protein were reduced and alkylated with 10 mM DTT and 20 mM IAA, respectively. Proteins were digested with LysC 0.04 AU/mg protein for 30 min at 37°C followed by the addition of 5% trypsin overnight at 37°C. The next day, proteins were further digested by the addition of 1% trypsin for one hour. Digested peptides acidified with 2% trifluoroacetic acid (TFA) and precipitated SDC was removed by centrifugation at 20,000 x g for 1 min. The supernatant was transferred to C18 stage tips (Thermo Fischer Scientific) for peptide purification. Peptides were eluted with 65% acetonitrile in 0.1% TFA, vacuum-dried, and resuspended in 50 µL of 0.1% formic acid. Approximately 1 µg of peptides in solvent A (0.1% formic acid) was loaded onto a 20-cm analytical column (100-µm inner diameter) packed with ReproSil-Pur C18 AQ 1.9-µm RP material using a Thermo Scientific™ Vanquish™ Neo UHPLC system coupled to an Orbitrap Astral mass spectrometer. Peptides were eluted from the column by changing the gradient from 98% solvent A and 2% solvent B (80% acetonitrile and 0.1% formic acid) to 38% solvent B during 45 min. Over the next 2 min, solvent B was increased to 90% and the column was washed for 6 more min. Mass spectrometer operated in a positive mode with orbitrap resolution set at 240,000, scan range between 400 and 1000 m/z and maximum injection time of 5 ms. Data-independent acquisition (DIA) was performed with higher-energy collision-induced dissociation set to 25%, a scan range from 150 to 2000 m/z, a maximum injection time of 3.5 ms, and a cycle time of 0.6 s.PrideNot availableRaw data files were searched with Spectronaut (20.0) using the Mus musculus reference proteome FASTA file, ID UP000000589_10090, containing 21,756 genes (downloaded on 17.06.2025). Most search settings were kept at their defaults. In brief, search settings included trypsin-digested peptides with a maximum of 2 missed cleavages, carbamidomethyl (C) as a fixed modification, and a maximum of 5 variable modifications, including acetyl (Protein N-term) and oxidation (M). The Deep DirectDIA+ workflow was used for identification. For quantification, precursor filtering was set to “Identified (Qvalue)”, and no imputation was performed. The proteotypicity filter was set to “Only protein group specific”, and cross-run normalization was usedProteomicsTrisha Jean GrevengoedPARTIALDepartment of Biomedical Sciences, University of CopenhagenMus Musculus (mouse)10.1016/J.JHEP.2026.04.033ibradic95@gmail.comUniversity of Southern DenmarkDenmarkfalseArachidonoyl-taurine protects from diet-induced hepatic steatosis and inflammation - Time course experimentMetabolic dysfunction-associated steatotic liver disease and its progressive and inflamma-tory form metabolic dysfunction-associated steatohepatitis represents a global health challenge, with limited treatment options available. In this study we identified an endoge-nous, understudied omega-6 fatty acid metabolite, arachidonoyl-taurine (ARA-T), capable of mitigating liver disease. ARA-T levels increased in human plasma of chronic and acute fatty liver and their abundance can be driven in humans and mice by dietary supplementa-tion of arachidonic acid. Surprisingly, our genetic model of elevated circulating ARA-T levels prevented inflammation and hepatic steatosis by increased uptake and turnover of fatty ac-ids in the liver. Pharmacological administration of ARA-T reduced liver weight and diet-induced hepatic lipid deposition in mice, demonstrating its potential to protect against and reverse the progression of liver disease. Thus, ARA-T may represent a way to protect against pro-inflammatory actions of omega-6 fatty acids thereby contributing to regulation of in-flammation and accumulation of hepatic lipids.2026-05-202026-03-10PXD075440NEWT:6945NEWT:3555NEWT:241368NEWT:2NEWT:157546NEWT:190802NEWT:35554NEWT:9778NEWT:150475NEWT:9417NEWT:7111NEWT:347515NEWT:1216979NEWT:307972NEWT:32046NEWT:544496NEWT:5180NEWT:256737NEWT:2042546NEWT:115104NEWT:1081927NEWT:67825NEWT:185579NEWT:43179NEWT:13076NEWT:1249668NEWT:317NEWT:55153NCBITaxon:10407NEWT:1736309NEWT:7227NEWT:7469NEWT:885318NEWT:1211601NEWT:415540NEWT:4081NEWT:876138NEWT:554NEWT:98334NEWT:426428NEWT:237561NEWT:6928NEWT:10036NEWT:7574NEWT:1351NEWT:7215NEWT:29204NEWT:272563NEWT:507601NCBITaxon:79857NCBITaxon:6157NEWT:95648NEWT:3885NEWT:746360NEWT:6239NEWT:3888NEWT:1589NEWT:470150NEWT:135622NEWT:216257NEWT:6915NEWT:9986NEWT:101510NEWT:4054NEWT:3880NEWT:272559NEWT:226186NEWT:3641NEWT:383379NEWT:8782NEWT:1263854NEWT:1000589NEWT:435590NEWT:1902NEWT:85962NEWT:160488NEWT:28104NEWT:317447NEWT:7955NCBITaxon:2NEWT:985076NEWT:7959NEWT:2261NEWT:4565NEWT:1264690NEWT:515619NEWT:6192NEWT:28532NCBITaxon:38727NEWT:34305NEWT:59729NCBITaxon:183674NEWT:224308NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:216595NEWT:243230NEWT:8355NEWT:1283NEWT:931281NEWT:4550NEWT:1000561NEWT:7029NEWT:1283300NEWT:6183NEWT:6063NEWT:334747NEWT:61235NCBITaxon:79824NEWT:4787NCBITaxon:4563NEWT:5755NEWT:3218NEWT:5759NEWT:1736231NEWT:436486NEWT:6287NEWT:2242NEWT:300641NEWT:4784NEWT:727NEWT:9796NEWT:11320NEWT:725NEWT:360106NEWT:260707NEWT:287NEWT:10117NEWT:10239NCBITaxon:6191NEWT:10116NEWT:1280NEWT:1836NEWT:1735272NEWT:83334NEWT:185431NEWT:83332NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:80863NEWT:44685NEWT:2697049NEWT:1148NEWT:11676NEWT:55571NEWT:100226NCBITaxon:6073NEWT:4530NEWT:4896NEWT:6279NEWT:1123869NEWT:7370NEWT:75058NEWT:83906NEWT:607699NEWT:6282NEWT:1094343NEWT:208964NEWT:1134506NEWT:575584NEWT:296543NEWT:1773NEWT:38783NEWT:8727NEWT:1895NEWT:4006NEWT:1182590NEWT:8726NEWT:6669NEWT:10090NEWT:935293NEWT:64152NEWT:749200NEWT:4120NEWT:51515NEWT:5693NEWT:8724NEWT:51511NEWT:92867NEWT:8723NEWT:990346NEWT:5334NEWT:145953NEWT:257309NEWT:100816NEWT:230741NEWT:284812NCBITaxon:10359NCBITaxon:1313NEWT:43330NEWT:242619NEWT:44544NEWT:632957NEWT:373995NEWT:5689NEWT:645463NEWT:544404NEWT:3702NEWT:129249NEWT:9925NEWT:8839NEWT:4232NEWT:990119NEWT:2758385NEWT:4113NEWT:837NEWT:11298NEWT:171101NEWT:421932NEWT:196627NEWT:408172NEWT:5691NEWT:408170NEWT:493760NEWT:260710NEWT:627025NEWT:400772NEWT:1097677NEWT:3708NEWT:128161NEWT:106592NEWT:536231NEWT:61674NEWT:1117957NEWT:9913NEWT:1432138NEWT:10312NEWT:1424507NEWT:4100NEWT:1076NEWT:6763NEWT:803NEWT:8030NEWT:29722NEWT:380394NEWT:1692259NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:180066NEWT:67767NEWT:46835NEWT:135588NEWT:1843183NEWT:95486NEWT:58002NEWT:9103NEWT:4577NEWT:1416333NEWT:5664NEWT:2157NEWT:146479NEWT:10306NCBITaxon:2759NEWT:1911079NEWT:8022NEWT:145943NCBITaxon:4932NEWT:595536NEWT:3635NEWT:5811NEWT:235443NEWT:1480154NEWT:1274414NEWT:27606NEWT:59202NEWT:9975NEWT:3197NEWT:9615NEWT:10299NEWT:860688NEWT:411483NEWT:884019NEWT:411490NEWT:169963NEWT:36329NEWT:1147787NCBITaxon:3044782NEWT:72407NEWT:476272NEWT:349741NEWT:9606NEWT:367830NEWT:157295NEWT:641501NEWT:178616NEWT:410289NEWT:373153NEWT:915099NEWT:74940NEWT:9721NEWT:1450511NEWT:360094NEWT:470NEWT:411901NEWT:1313NEWT:411469NEWT:84023NEWT:9838NCBITaxon:9615NCBITaxon:5811NEWT:58334NEWT:411464NEWT:1193501NEWT:3055NEWT:6326NEWT:6689NEWT:411460NEWT:2762NEWT:5476NEWT:1174673NEWT:562NEWT:411470NEWT:33952NEWT:2094720NEWT:1274432NEWT:1274426NEWT:1423NEWT:4932NEWT:70448NEWT:9825NEWT:1274423NEWT:3603NEWT:698936NEWT:2759NEWT:3847NEWT:39946NEWT:9823NEWT:178876NEWT:9940NEWT:327160NEWT:573NEWT:521001NEWT:9031NEWT:1274420NEWT:7091NEWT:578458