{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/checksum.txt"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/PD018LPSc_N_7_Slot2-5_1_10693.d.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/PD018LPSc_N_4_Slot2-4_1_10691.d.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/PD018LPSc_BLM_3_Slot2-7_1_10697.d.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/PD018LPSc_BLM_18_Slot2-6_1_10695.d.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/PD018LPSc_N_3_Slot2-3_1_10689.d.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/PD018LPSc_BLM_25_Slot2-8_1_10699.d.raw"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075455/txt.rar"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["songxd71@bzmc.edu.cn"],"submitter":["Du Guanglian"],"technology_type":["Data-dependent acquisition","Mass Spectrometry"],"software":[""],"submitter_keywords":["succinyl-proteome","hdac8","cytoskeleton remodeling","Idiopathic pulmonary fibrosis","Idiopathic pulmonary fibrosis; lysine succinylation; succinyl-proteome; capzb; hdac8; cytoskeleton remodeling; lc-ms/ms; mouse lung tissue","lysine succinylation","capzb","mouse lung tissue","lc-ms/ms"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD075455"],"tissue":["Lung"],"sample_protocol":["Cells were harvested and lysed using a urea-based lysis buffer supplemented with protease inhibitors and desuccinylase inhibitors. Total protein was extracted and quantified using a BCA assay. Proteins were reduced, alkylated, and digested with trypsin. Peptides were subjected to affinity enrichment using anti-succinyl-lysine antibody beads to enrich lysine-succinylated peptides. Enriched peptides were desalted and prepared for LC-MS/MS analysis."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["Raw mass spectrometry data were processed using MaxQuant software. Database searches were performed against the UniProt mouse protein database with carbamidomethylation of cysteine as a fixed modification and succinylation (lysine) and oxidation (methionine) as variable modifications. Trypsin was specified as the digestion enzyme with up to two missed cleavages allowed. Peptide and protein false discovery rates (FDR) were controlled at 1%. Quantitative analysis was performed based on normalized peptide intensities."],"omics_type":["Proteomics"],"labhead":["XiaoDong Song"],"instrument_platform":[""],"labhead_affiliation":["Binzhou Medical University"],"submission_type":["PARTIAL"],"species":["Mus Musculus (mouse)"],"submitter_mail":["d2435186757@gmail.com"],"publication":["42050207 Liu B, Kang D, Zhang J, Wang Y, Li R, Lv C, Zhai N, Song X, Zhang S, Li H. HDAC8-mediated CAPZB desuccinylation enhances cytoskeleton remodeling to promote idiopathic pulmonary fibrosis. Commun Biol. 2026 10.1038/s42003-026-10125-y"],"submitter_affiliation":["Binzhou Medical University"],"submitter_country":["China"],"pubmed_abstract":["Idiopathic pulmonary fibrosis (IPF) is a chronic progressive lung disease of unknown etiology, characterized by progressive scarring and fibrosis of the lungs, leading to irreversible loss of lung function. Current mechanism studies have found that fibroblasts, as the main effector cells, exhibit abnormal differentiation and proliferation in pulmonary fibrosis, thereby promoting collagen secretion for extracellular matrix (ECM) deposition. Cytoskeletal remodeling is the basis for fibroblast-to-myofibroblast differentiation, however, the key proteins and regulatory mechanisms involved in this process are still unclear. Here, F-actin-capping protein subunit beta (CAPZB) was identified to be desuccinylated at three lysine residues (K57, K95, and K235). Gain- and loss-of-function studies in vitro and in vivo demonstrated that CAPZB regulates cytoskeletal remodeling and differentiation of fibroblasts to myofibroblasts by capping the barbed end of F-actin, and mutation of the modification site confirmed the inhibition of CAPZB function by desuccinylation. Further studies showed that histone deacetylase enzymes 8 (HDAC8) could interacts with CAPZB, and its pro-fibrotic role as a desuccinylase was verified. Our findings suggested that desuccinylation inhibited CAPZB function and promoted cytoskeletal remodeling, targeting the succinylation modification of CAPZB could potentially serve as a novel therapeutic approach for pulmonary fibrosis."],"pubmed_title":["HDAC8-mediated CAPZB desuccinylation enhances cytoskeleton remodeling to promote idiopathic pulmonary fibrosis."],"pubmed_authors":["Liu Bo B, Kang Di D, Zhang Jinjin J, Wang Yujie Y, Li Rongrong R, Lv Changjun C, Zhai Nailiang N, Song Xiaodong X, Zhang Songzi S, Li Hongbo H"],"additional_accession":[]},"is_claimable":false,"name":"Succinyl-proteome profiling reveals HDAC8-mediated CAPZB desuccinylation regulates cytoskeleton remodeling in bleomycin-induced idiopathic pulmonary fibrosis","description":"Idiopathic pulmonary fibrosis (IPF) is a chronic progressive lung disease of unknown etiology, characterized by progressive scarring and fibrosis of the lungs, leading to irreversible loss of lung function. Current mechanism studies have found that fibroblasts, as the main effector cells, exhibit abnormal differentiation and proliferation in pulmonary fibrosis, thereby promoting collagen secretion for extracellular matrix (ECM) deposition. Cytoskeletal remodeling is the basis for fibroblast-to-myofibroblast differentiation, however, the key proteins and regulatory mechanisms involved in this process are still unclear. Here, F-actin-capping protein subunit beta (CAPZB) was identified to be desuccinylated at three lysine residues (K57, K95, and K235). Gain- and loss-of-function studies in vitro and in vivo demonstrated that CAPZB regulates cytoskeletal remodeling and differentiation of fibroblasts to myofibroblasts by capping the barbed end of F-actin, and mutation of the modification site confirmed the inhibition of CAPZB function by desuccinylation. Further studies showed that histone deacetylase enzymes 8 (HDAC8) could interacts with CAPZB, and its pro-fibrotic role as a desuccinylase was verified. Our findings suggested that desuccinylation inhibited CAPZB function and promoted cytoskeletal remodeling, targeting the succinylation modification of CAPZB could potentially serve as a novel therapeutic approach for pulmonary fibrosis.","dates":{"publication":"2026-04-30","submission":"2026-03-10"},"accession":"PXD075455","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:3555","NEWT:241368","NEWT:2","NEWT:157546","NEWT:190802","NEWT:35554","NEWT:9778","NEWT:150475","NEWT:9417","NEWT:7111","NEWT:347515","NEWT:1216979","NEWT:307972","NEWT:32046","NEWT:544496","NEWT:5180","NEWT:256737","NEWT:2042546","NEWT:115104","NEWT:1081927","NEWT:67825","NEWT:185579","NEWT:43179","NEWT:13076","NEWT:1249668","NEWT:317","NEWT:55153","NCBITaxon:10407","NEWT:1736309","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:415540","NEWT:4081","NEWT:876138","NEWT:554","NEWT:98334","NEWT:426428","NEWT:237561","NEWT:6928","NEWT:10036","NEWT:7574","NEWT:1351","NEWT:7215","NEWT:29204","NEWT:272563","NEWT:507601","NCBITaxon:79857","NCBITaxon:6157","NEWT:95648","NEWT:3885","NEWT:746360","NEWT:6239","NEWT:3888","NEWT:1589","NEWT:470150","NEWT:135622","NEWT:216257","NEWT:6915","NEWT:9986","NEWT:101510","NEWT:4054","NEWT:3880","NEWT:3641","NEWT:383379","NEWT:8782","NEWT:1263854","NEWT:1000589","NEWT:1902","NEWT:85962","NEWT:160488","NEWT:28104","NEWT:317447","NEWT:7955","NCBITaxon:2","NEWT:985076","NEWT:7959","NEWT:2261","NEWT:4565","NEWT:1264690","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:216595","NEWT:243230","NEWT:8355","NEWT:1283","NEWT:931281","NEWT:4550","NEWT:1000561","NEWT:7029","NEWT:1283300","NEWT:6183","NEWT:6063","NEWT:334747","NEWT:61235","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:436486","NEWT:6287","NEWT:2242","NEWT:300641","NEWT:4784","NEWT:727","NEWT:9796","NEWT:725","NEWT:360106","NEWT:260707","NEWT:287","NEWT:10117","NEWT:10239","NCBITaxon:6191","NEWT:10116","NEWT:1280","NEWT:1836","NEWT:1735272","NEWT:83334","NEWT:185431","NEWT:83332","NEWT:29760","NEWT:260704","NEWT:703612","NEWT:260705","NEWT:80863","NEWT:44685","NEWT:2697049","NEWT:1148","NEWT:11676","NEWT:55571","NEWT:100226","NCBITaxon:6073","NEWT:4530","NEWT:4896","NEWT:6279","NEWT:1123869","NEWT:7370","NEWT:75058","NEWT:83906","NEWT:607699","NEWT:6282","NEWT:1094343","NEWT:208964","NEWT:1134506","NEWT:575584","NEWT:296543","NEWT:1773","NEWT:38783","NEWT:8727","NEWT:1895","NEWT:4006","NEWT:1182590","NEWT:8726","NEWT:6669","NEWT:10090","NEWT:935293","NEWT:64152","NEWT:749200","NEWT:4120","NEWT:51515","NEWT:5693","NEWT:8724","NEWT:51511","NEWT:92867","NEWT:8723","NEWT:990346","NEWT:5334","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:230741","NEWT:284812","NCBITaxon:10359","NCBITaxon:1313","NEWT:43330","NEWT:242619","NEWT:44544","NEWT:632957","NEWT:373995","NEWT:645463","NEWT:544404","NEWT:3702","NEWT:129249","NEWT:9925","NEWT:8839","NEWT:4232","NEWT:990119","NEWT:2758385","NEWT:4113","NEWT:837","NEWT:11298","NEWT:171101","NEWT:421932","NEWT:196627","NEWT:408172","NEWT:5691","NEWT:408170","NEWT:493760","NEWT:260710","NEWT:627025","NEWT:400772","NEWT:1097677","NEWT:3708","NEWT:128161","NEWT:106592","NEWT:61674","NEWT:1117957","NEWT:9913","NEWT:1432138","NEWT:10312","NEWT:1424507","NEWT:4100","NEWT:1076","NEWT:6763","NEWT:803","NEWT:8030","NEWT:29722","NEWT:380394","NEWT:1692259","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:180066","NEWT:67767","NEWT:46835","NEWT:135588","NEWT:1843183","NEWT:95486","NEWT:58002","NEWT:9103","NEWT:4577","NEWT:1416333","NEWT:5664","NEWT:2157","NEWT:146479","NEWT:10306","NCBITaxon:2759","NEWT:1911079","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NEWT:3635","NEWT:5811","NEWT:1480154","NEWT:235443","NEWT:1274414","NEWT:27606","NEWT:59202","NEWT:9975","NEWT:3197","NEWT:9615","NEWT:10299","NEWT:860688","NEWT:884019","NEWT:169963","NEWT:36329","NEWT:1147787","NCBITaxon:3044782","NEWT:72407","NEWT:9606","NEWT:367830","NEWT:157295","NEWT:641501","NEWT:178616","NEWT:410289","NEWT:373153","NEWT:915099","NEWT:74940","NEWT:9721","NEWT:1450511","NEWT:360094","NEWT:470","NEWT:1313","NEWT:84023","NEWT:9838","NCBITaxon:9615","NCBITaxon:5811","NEWT:58334","NEWT:1193501","NEWT:3055","NEWT:6326","NEWT:6689","NEWT:2762","NEWT:5476","NEWT:1174673","NEWT:562","NEWT:33952","NEWT:2094720","NEWT:1274432","NEWT:1274426","NEWT:1423","NEWT:4932","NEWT:70448","NEWT:9825","NEWT:1274423","NEWT:3603","NEWT:698936","NEWT:2759","NEWT:3847","NEWT:39946","NEWT:9823","NEWT:178876","NEWT:9940","NEWT:327160","NEWT:573","NEWT:9031","NEWT:1274420","NEWT:7091","NEWT:578458"],"pubmed":["42050207"],"ORCID":["0009-0008-7162-8205"]}}