{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/proteinGroups.txt"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrH3_612.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrC1_589.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrC4_618.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrL1_591.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrC3_601.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrC2_595.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrH2_599.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrH1_593.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrL3_603.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrL2_597.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075471/LrL4_609.d.zip"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"labhead_mail":["natalie.leys@sckcen.be"],"submitter":["Surya Gupta"],"technology_type":["Data-dependent acquisition","Mass Spectrometry","Bottom-up proteomics"],"software":[""],"submitter_keywords":[""],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD075471"],"sample_protocol":["The protein extraction, quantification as well as the proteomic analysis were performed as previously described in Ellena et al. 2024. The cell pellets were resuspended in twice their volume of 2% SDS in 50 mM ammonium bicarbonate (ABC). After vortexing, the suspension was incubated at 95 °C for 5 min and subsequently cooled on ice for 5 min. Cell lysis was then performed by sonication (4 × 10 s, amplitude 40%, 1 cycle, on ice) using a sonicator (Imlab, Boutersem, Belgium). The samples were centrifuged, and the supernatant was collected for protein quantification using a BCA assay (Merck Life Science BV, Hoeilaart, Belgium) according to the manufacturer’s instructions. Extracted proteins were further processed using the suspension trapping method, S-trap (Bioconnect, Huissen, The Netherlands) following the manufacturer’s instructions. LC-MS/MS analysis was performed using a nanoElute UHPLC (Bruker Daltonics, Bremen, Germany) connected to a QTOF-MS instrument (Impact II, Bruker Daltonics, Germany) via a CaptiveSpray nanoflow electrospray source (Bruker Daltonics, Bremen, Germany). Ellena, G. et al. Development and implementation of a simulated microgravity setup for edible cyanobacteria. NPJ Microgravity 10, 99 (2024)."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["All raw mass spectrometry spectra files were processed using MaxQuant software version 2.7.0 (Max-Plank Institute of Biochemistry, Department of Proteomics and Signal Transduction, Munich, Germany) and proteins were identified with the built-in Andromeda search engine. In total 12 raw files were processed in parallel. The MS/MS spectra searches were performed with a database containing all Limosilactobacillus reuteri strain DSM20016  (Taxonomy ID: 55743) UniProt protein sequences (downloaded from ftp.uniprot.org on 2025-12-17). Searches were performed with default MaxQuant parameter settings with cysteine carbamidomethylation as fixed modification, and methionine oxidation and Protein N-terminal acetylation as variable modification. False-discovery rate (FDR) cutoffs were set to 1% on peptide, protein and site decoy level, trypsin as a digestion enzyme and seven amino acids as minimum peptide length. The resulting data from MaxQuant with minimum two unique peptides was retained, processed to remove reverse hits and contaminants, and log2 transformed for further analysis. The technical variation among replicates was removed by median normalization on log2-transformed intensities via an in-house python script. To impute the small number of missing values in the data, downshift imputation method was used. Protein differential expression was calculated using LIMMA R package, based on the empirical Bayes moderated test-statistics. Differential expression was considered significant at a q-value < 0.05 and a fold-change (FC) of at least ± 1.5."],"omics_type":["Proteomics"],"labhead":["natalie leys"],"instrument_platform":[""],"labhead_affiliation":["Head of Microbiology Unit, SCKCEN"],"submission_type":["PARTIAL"],"species":["Lactobacillus Reuteri Dsm 20016"],"publication":["Not available"],"submitter_mail":["surya.gupta@sckcen.be"],"submitter_affiliation":["SCKCEN"],"submitter_country":["Belgium"],"additional_accession":[]},"is_claimable":false,"name":"Limosilactobacillus reuteri DSM 20016 under simulated martian radiation","description":"Future crewed missions and long-term settlements on Mars will require robust microbial systems capable of operating under chronic low dose rate ionizing radiation. Microorganisms are key candidates for bioregenerative life-support applications, including nutrient production, fermentation, and metabolic recycling. However, little is known about how beneficial microbial species commonly used in food biotechnology perform under Mars-relevant radiation levels. This study evaluated the radiation tolerance, growth stability, vitamin biosynthesis, and whole-proteome response of Limosilactobacillus reuteri, under simulated Martian ionizing radiation environment. Cultures were exposed to 21 and 77 µSv h⁻¹ using 60Co (agar cultures). L. reuteri exhibited clear radiation sensitivity on agar, with reduced biomass and altered lag phase. However, in long-term liquid culture under 137Cs irradiation, the strain remained viable and productive, displaying only subtle trends toward lower generation time, stronger acidification, reduced CO2 production, decreased riboflavin secretion, and increased intracellular cobalamin.","dates":{"publication":"2026-09-30","submission":"2026-03-11"},"accession":"PXD075471","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:3555","NEWT:241368","NEWT:2","NEWT:157546","NEWT:190802","NEWT:35554","NEWT:9778","NEWT:150475","NEWT:9417","NEWT:347515","NEWT:1216979","NEWT:307972","NEWT:32046","NEWT:544496","NEWT:5180","NEWT:256737","NEWT:2042546","NEWT:115104","NEWT:1081927","NEWT:67825","NEWT:185579","NEWT:43179","NEWT:13076","NEWT:1249668","NEWT:376741","NEWT:317","NEWT:55153","NCBITaxon:10407","NEWT:1736309","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:415540","NEWT:4081","NEWT:876138","NEWT:554","NEWT:98334","NEWT:426428","NEWT:237561","NEWT:6928","NEWT:10036","NEWT:7574","NEWT:1351","NEWT:7215","NEWT:29204","NEWT:272563","NEWT:79220","NEWT:507601","NCBITaxon:79857","NCBITaxon:6157","NEWT:95648","NEWT:3885","NEWT:746360","NEWT:6239","NEWT:3888","NEWT:1589","NEWT:470150","NEWT:135622","NEWT:216257","NEWT:6915","NEWT:9986","NEWT:101510","NEWT:4054","NEWT:3880","NEWT:3641","NEWT:383379","NEWT:8782","NEWT:1263854","NEWT:1000589","NEWT:1902","NEWT:85962","NEWT:160488","NEWT:28104","NEWT:317447","NEWT:7955","NCBITaxon:2","NEWT:985076","NEWT:7959","NEWT:2261","NEWT:623","NEWT:4565","NEWT:1264690","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:211586","NEWT:216595","NEWT:243230","NEWT:8355","NEWT:1283","NEWT:931281","NEWT:1000561","NEWT:9685","NEWT:7029","NEWT:1283300","NEWT:6183","NEWT:6063","NEWT:334747","NEWT:61235","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:436486","NEWT:6287","NEWT:2242","NEWT:300641","NEWT:4784","NEWT:727","NEWT:9796","NEWT:725","NEWT:360106","NEWT:260707","NEWT:287","NEWT:10117","NEWT:10239","NCBITaxon:6191","NEWT:10116","NEWT:1280","NEWT:1836","NEWT:1735272","NEWT:83334","NEWT:185431","NEWT:83332","NEWT:29760","NEWT:260704","NEWT:703612","NEWT:260705","NEWT:80863","NEWT:44685","NEWT:2697049","NEWT:1148","NEWT:11676","NEWT:55571","NEWT:100226","NCBITaxon:6073","NEWT:4530","NEWT:4896","NEWT:6279","NEWT:1123869","NEWT:7370","NEWT:75058","NEWT:83906","NEWT:607699","NEWT:6282","NEWT:426688","NEWT:1094343","NEWT:208964","NEWT:1134506","NEWT:575584","NEWT:296543","NEWT:1773","NEWT:38783","NEWT:8727","NEWT:1895","NEWT:1182590","NEWT:8726","NEWT:6669","NEWT:10090","NEWT:935293","NEWT:64152","NEWT:749200","NEWT:4120","NEWT:51515","NEWT:5693","NEWT:8724","NEWT:51511","NEWT:92867","NEWT:8723","NEWT:990346","NEWT:5334","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:230741","NEWT:284812","NCBITaxon:10359","NCBITaxon:1313","NEWT:43330","NEWT:242619","NEWT:44544","NEWT:632957","NEWT:373995","NEWT:5689","NEWT:645463","NEWT:544404","NEWT:3702","NEWT:129249","NEWT:9925","NEWT:8839","NEWT:4232","NEWT:990119","NEWT:2758385","NEWT:4113","NEWT:837","NEWT:11298","NEWT:171101","NEWT:421932","NEWT:196627","NEWT:408172","NEWT:5691","NEWT:408170","NEWT:493760","NEWT:260710","NEWT:627025","NEWT:400772","NEWT:1097677","NEWT:3708","NEWT:128161","NEWT:106592","NEWT:1117957","NEWT:9913","NEWT:1432138","NEWT:10312","NEWT:1424507","NEWT:4100","NEWT:1076","NEWT:6763","NEWT:3498","NEWT:803","NEWT:8030","NEWT:29722","NEWT:380394","NEWT:1692259","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:180066","NEWT:67767","NEWT:46835","NEWT:135588","NEWT:1843183","NEWT:95486","NEWT:58002","NEWT:9103","NEWT:4577","NEWT:1416333","NEWT:5664","NEWT:2157","NEWT:749906","NEWT:146479","NEWT:10306","NCBITaxon:2759","NEWT:1911079","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NCBITaxon:620","NEWT:3635","NEWT:5811","NEWT:235443","NEWT:1480154","NEWT:1274414","NEWT:27606","NEWT:59202","NEWT:9975","NEWT:3197","NEWT:9615","NEWT:10299","NEWT:2719099","NEWT:860688","NEWT:884019","NEWT:169963","NEWT:36329","NEWT:1147787","NCBITaxon:3044782","NEWT:72407","NEWT:9606","NEWT:367830","NEWT:157295","NEWT:641501","NEWT:178616","NEWT:410289","NEWT:373153","NEWT:915099","NEWT:74940","NEWT:1450511","NEWT:360094","NEWT:470","NEWT:84023","NEWT:9838","NCBITaxon:9615","NEWT:58334","NEWT:1193501","NEWT:3055","NEWT:6326","NEWT:6689","NEWT:2762","NEWT:5476","NEWT:1174673","NEWT:562","NEWT:33952","NEWT:1274432","NEWT:1274426","NEWT:1423","NEWT:4932","NEWT:70448","NEWT:9825","NEWT:1274423","NEWT:3603","NEWT:698936","NEWT:2759","NEWT:3847","NEWT:39946","NEWT:9823","NEWT:178876","NEWT:9940","NEWT:327160","NEWT:573","NEWT:9031","NEWT:1274420","NEWT:7091","NEWT:578458"],"ORCID":["0000-0002-6290-6161"]}}