{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/checksum.txt"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/D3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/W3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/D4.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/W4.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/D1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/W2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/D2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/W1.raw"],"Mztab":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075513/LFQ_Turno262376.mzTab"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["adekanty@ial.unl.edu.ar"],"submitter":["Andrés Dekanty"],"technology_type":["Mass Spectrometry","Bottom-up proteomics"],"software":[""],"submitter_keywords":["Oxidative stress","Adipose tissue","Inter organ communication","Mirna","Insulin like peptides"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD075513"],"tissue":["Fat Body"],"sample_protocol":["We compared fat body samples from four biological replicates of wild-type (w1118) and dcr-1MI13376 heterozygous larvae. Fat bodies were dissected and processed for proteomic analysis. Peptides were separated on a nanoHPLC Ultimate 3000 system (Thermo Scientific) using an EASY-Spray ES903 column (50 cm × 50 μm ID, PepMap RSLC C18) at a flow rate of 300 nL/min. Solvent A consisted of 0.1% formic acid in water, and solvent B of 0.1% formic acid in acetonitrile. The gradient was as follows: 4–30% B for 114 min, 30–80% B for 14 min, and 80% B for 2 min. Mass spectrometry was performed on a Q-Exactive HF (Thermo Scientific) with the following settings: full scan range m/z 375–2000, resolution 120,000 (at m/z 200), AGC target 1 × 10^6, and maximum injection time 100 ms. The 15 most intense ions were selected for MS/MS fragmentation using a normalized collision energy of 28 eV. MS/MS scans were acquired at 30,000 resolution with an AGC target of 5 × 10^5, intensity threshold 1.5 × 10^5, isolation window 1.4 m/z, and maximum injection time 55 ms. Singly charged, unassigned, and ≥6+ ions were excluded, and dynamic exclusion was set to 30 s."],"repository":["Pride"],"quantification_method":[""],"modification":[""],"data_protocol":["MS data were analyzed using Proteome Discoverer v2.4.1.15 with standard workflows. Spectrawere searched against the Drosophila melanogaster UNIPROT database (UP000000803) with precursor and fragment mass tolerances of 10 ppm and 0.02 Da, respectively, allowing up to twomissed cleavages."],"omics_type":["Proteomics"],"labhead":["Andres Dekanty"],"instrument_platform":[""],"labhead_affiliation":["Laboratorio de Biología Molecular - Instituto de Agrobiotecnología del Litoral - CONICET - Santa Fe,Argentina"],"submission_type":["COMPLETE"],"species":["Drosophila Melanogaster (fruit Fly)"],"submitter_mail":["adekanty@ial.unl.edu.ar"],"publication":["10.1073/PNAS.2525327123"],"submitter_affiliation":["CONICET"],"submitter_country":["Argentina"],"doi":["10.6019/PXD075513"],"additional_accession":[]},"is_claimable":false,"name":"Adipose Dicer-1 modulates systemic insulin signaling and longevity via a miR-8–Aop–Dilp6 axis","description":"This project examines how adipose tissue regulates systemic metabolism and aging in Drosophila melanogaster through the miRNA-processing enzyme Dicer-1 (Dcr-1). Proteomic profiling of fat bodies from Dcr-1 heterozygous flies was performed to identify molecular changes associated with reduced Dcr-1 activity. The dataset reveals metabolic and stress-response remodeling consistent with reduced insulin/IGF signaling, supporting a model in which decreased Dcr-1 increases Dilp6 expression in the fat body and suppresses Dilp2 secretion from brain insulin-producing cells, promoting longevity.","dates":{"publication":"2026-04-13","submission":"2026-03-11"},"accession":"PXD075513","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:3555","NEWT:241368","NEWT:2","NEWT:157546","NEWT:190802","NEWT:35554","NEWT:150475","NEWT:9417","NEWT:347515","NEWT:1216979","NEWT:307972","NEWT:544496","NEWT:5180","NEWT:256737","NEWT:115104","NEWT:1081927","NEWT:67825","NEWT:13076","NEWT:1249668","NEWT:317","NEWT:1736309","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:4081","NEWT:876138","NEWT:554","NEWT:98334","NEWT:237561","NEWT:10036","NEWT:7574","NEWT:1351","NEWT:7215","NEWT:272563","NEWT:507601","NCBITaxon:79857","NCBITaxon:6157","NEWT:95648","NEWT:746360","NEWT:6239","NEWT:1589","NEWT:470150","NEWT:135622","NEWT:216257","NEWT:6915","NEWT:9986","NEWT:101510","NEWT:4054","NEWT:3880","NEWT:8782","NEWT:1000589","NEWT:1902","NEWT:85962","NEWT:160488","NEWT:28104","NEWT:317447","NEWT:7955","NCBITaxon:2","NEWT:985076","NEWT:7959","NEWT:2261","NEWT:4565","NEWT:1264690","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:216595","NEWT:243230","NEWT:8355","NEWT:931281","NEWT:7029","NEWT:1283300","NEWT:334747","NCBITaxon:79824","NCBITaxon:4563","NEWT:5755","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:436486","NEWT:6287","NEWT:2242","NEWT:300641","NEWT:4784","NEWT:727","NEWT:9796","NEWT:725","NEWT:360106","NEWT:260707","NEWT:287","NEWT:10117","NEWT:10239","NEWT:10116","NEWT:1280","NEWT:1836","NEWT:1735272","NEWT:83334","NEWT:83332","NEWT:29760","NEWT:703612","NEWT:260705","NEWT:80863","NEWT:2697049","NEWT:1148","NEWT:11676","NEWT:55571","NEWT:100226","NCBITaxon:6073","NEWT:4530","NEWT:4896","NEWT:6279","NEWT:7370","NEWT:6282","NEWT:1134506","NEWT:575584","NEWT:1773","NEWT:38783","NEWT:8727","NEWT:1895","NEWT:1182590","NEWT:8726","NEWT:10090","NEWT:935293","NEWT:749200","NEWT:4120","NEWT:5693","NEWT:8724","NEWT:51511","NEWT:92867","NEWT:8723","NEWT:990346","NEWT:5334","NEWT:145953","NEWT:257309","NEWT:230741","NEWT:284812","NCBITaxon:10359","NCBITaxon:1313","NEWT:43330","NEWT:242619","NEWT:44544","NEWT:373995","NEWT:544404","NEWT:3702","NEWT:129249","NEWT:8839","NEWT:4232","NEWT:990119","NEWT:4113","NEWT:837","NEWT:11298","NEWT:171101","NEWT:196627","NEWT:408172","NEWT:5691","NEWT:408170","NEWT:493760","NEWT:260710","NEWT:627025","NEWT:400772","NEWT:1097677","NEWT:3708","NEWT:106592","NEWT:9913","NEWT:1432138","NEWT:10312","NEWT:4100","NEWT:1076","NEWT:6763","NEWT:803","NEWT:8030","NEWT:29722","NEWT:380394","NEWT:1692259","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:67767","NEWT:135588","NEWT:1843183","NEWT:95486","NEWT:58002","NEWT:9103","NEWT:4577","NEWT:5664","NEWT:2157","NEWT:146479","NEWT:10306","NEWT:1911079","NEWT:145943","NEWT:3635","NEWT:5811","NEWT:235443","NEWT:1480154","NEWT:1274414","NEWT:3197","NEWT:9615","NEWT:10299","NEWT:860688","NEWT:884019","NEWT:169963","NEWT:36329","NEWT:9606","NEWT:367830","NEWT:157295","NEWT:410289","NEWT:373153","NEWT:915099","NEWT:74940","NEWT:1450511","NEWT:470","NEWT:84023","NEWT:9838","NCBITaxon:9615","NEWT:1193501","NEWT:3055","NEWT:6326","NEWT:6689","NEWT:2762","NEWT:5476","NEWT:1174673","NEWT:562","NEWT:1274432","NEWT:1274426","NEWT:1423","NEWT:4932","NEWT:70448","NEWT:9825","NEWT:1274423","NEWT:3603","NEWT:698936","NEWT:2759","NEWT:3847","NEWT:39946","NEWT:9823","NEWT:9940","NEWT:573","NEWT:9031","NEWT:1274420","NEWT:7091"],"pubmed":[],"ORCID":["0000-0002-0587-0449"]}}