{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/H37Ra_report.pg_matrix.tsv","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_report.tsv","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/H37Ra_report.pr_matrix.tsv"],"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/checksum.txt"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T1_0xMIC_3.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Mtb_0xMIC_3.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T2_HalfxMIC_1.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Mtb_HalfxMIC_2.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T2_HalfxMIC_2.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_Limma_script.r","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T2_HalfxMIC_3.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Mtb_HalfxMIC_3.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T1_0xMIC_1.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T2_0xMIC_1.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Mtb_0xMIC_1.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T1_HalfxMIC_2.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T2_0xMIC_2.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Mtb_HalfxMIC_1.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T2_0xMIC_3.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T1_HalfxMIC_3.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Mtb_0xMIC_2.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_T1_0xMIC_2.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD075873/Msm_Report_analysis_script.R"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["tariq.ganief@uct.ac.za"],"submitter":["Blake Stuart"],"technology_type":["Mass Spectrometry","Data-independent acquisition"],"disease":["Tuberculosis"],"software":[""],"submitter_keywords":[""],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD075873"],"sample_protocol":["Mycobacterium smegmatis mc²155 and Mycobacterium tuberculosis H37Ra were cultured in Middlebrook 7H9 medium supplemented with 10% OADC, 0.2% glycerol, and 0.05% Tween-80 at 37 °C with agitation. Rifampicin minimum inhibitory concentrations (MICs) were determined using microbroth dilution assays. For proteomic experiments, M. smegmatis cultures were grown to mid-exponential phase (OD₆₀₀ ≈ 1.0–1.2) and treated with either vehicle control (DMSO) or half-MIC rifampicin (3.9 µg/mL). Cells were harvested at 45 min and 180 min post-treatment in biological triplicate. M. tuberculosis H37Ra cultures were treated with half-MIC rifampicin (0.0006 µg/mL) and harvested after 2 h. Cultures were pelleted by centrifugation at 4000 rpm for 10 min at 4 °C, washed three times with phosphate-buffered saline, and stored at −20 °C. Pellets were lysed using trifluoroacetic acid (TFA), followed by neutralization with 4 M Tris buffer. Protein concentrations were quantified using a Bradford assay. Five micrograms of protein per sample were reduced with dithiothreitol, alkylated with iodoacetamide, and digested overnight with sequencing-grade trypsin. Peptides were desalted using EvoTips prior to LC–MS/MS analysis. Peptide samples were analysed using an Evosep One system coupled to a timsTOF Pro2 mass spectrometer operating in data-independent acquisition parallel accumulation–serial fragmentation (DIA-PASEF) mode. Peptides were separated using the Evosep 60 samples-per-day method and analysed across a mass range of 475–1000 m/z and an ion mobility range of 0.85–1.27 1/K₀. DIA-PASEF window settings included 25 Da mass width with 21 mass steps per cycle."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["The M. smegmatis (UniProt proteome ID: UP000000757) and M. tuberculosis H37Rv (UniProt proteome ID: UP000001584) reference proteomes were used for library free analysis in DIA-NN (v1.9). Missed cleavages were set to 1, with carbamidomethylation of cysteine as a fixed modification and no variable modifications. Additional parameters included peptide lengths of 7 - 30 amino acids, precursor charge range of 2 - 3, precursor m/z range of 300 - 1300 and fragment ion m/z range of 100 – 1700, with MS mass accuracy set to 20 ppm. Match-between-runs (MBR), heuristic protein inference, no shared spectra and double-pass mode were enabled. For M. smegmatis, downstream quantitative matrices (protein group and precursor-level) were generated by custom filtering of the DIA-NN report output using an in-house R script. In contrast, for M. tuberculosis H37Ra, the DIA-NN protein group (pg) and precursor (pr) matrices were used for downstream analysis.  Statistical analyses were performed using Perseus, MetaboAnalyst, R (v4.4.2) and Python (v3.14.1). The packages used to generate plots included ggplot, pheatmap, seaborn and matpotlib. Significantly dysregulated proteins between sample groups in M. smegmatis were identified using limma (FDR<0.05) differential expression analysis in R (v4.4.2) with readxl, limma and openxlsx packages. In M. tuberculosis H37Ra, Metaboanalyst was used to perform t-tests (FDR<0.05) and calculate fold-changes. Following statistical analyses, UniProt and Mycobrowser were used for protein annotation. Protein-protein interaction networks and functional enrichment analyses were performed using STRING, with enrichment clusters identified using Markov Cluster (MCL) algorithm and visualised in Cytoscape (v3.10.3)."],"omics_type":["Proteomics"],"labhead":["Tariq Ganief"],"instrument_platform":[""],"labhead_affiliation":["Department of Integrative Biomedical Sciences, University of Cape Town, Cape Town, South Africa, 7925"],"submission_type":["PARTIAL"],"species":["Mycobacterium Smegmatis (strain Atcc 700084 / Mc(2)155)","Bacteria","Mycobacterium Tuberculosis H37ra"],"publication":["42048180 Stuart BD, van der Merwe A, Chengalroyen MD, Moosa A, Warner DF, Blackburn JM, Ganief TA. Beyond Inhibition: Sublethal Rifampicin-Induced Molecular Adaptations Confer Phenotypic Drug Tolerance in Mycobacteria. ACS Infect Dis. 2026 12(5):1600-1610 10.1021/acsinfecdis.5c00701"],"submitter_mail":["strbla002@myuct.ac.za"],"submitter_affiliation":["University of Cape Town"],"submitter_country":["South Africa"],"pubmed_abstract":["Tuberculosis (TB) remains a major global health threat, largely due to <i>Mycobacterium tuberculosis</i> (Mtb) resilience, which can be exacerbated by exposure to sublethal antibiotic concentrations arising from factors such as patient nonadherence and the granuloma structure which limits drug penetration. Within host granulomas, Mtb can exhibit both phenotypic tolerance and genotypic resistance, complicating curative treatments. This study aimed to determine whether sublethal rifampicin acts as a signaling molecule in <i>Mycobacterium smegmatis</i> (Msm) and the attenuated Mtb H37Ra strain, triggering phenotypic changes that promote tolerance to lethal drug levels. Msm exposed to half-MIC rifampicin showed an initial transient growth deceleration followed by a resumption of proliferation, indicating the acquisition of phenotypic tolerance. Deep data-independent acquisition (DIA) mass spectrometry-based proteomic profiling revealed that the early response (45 min) involved the upregulation of ribosomal proteins, DNA replication, and de novo purine biosynthesis. Proteins associated with phenotypic resistance (e.g., RpoZ, GidB, WhiB2) and efflux transporters were also upregulated. As Msm recovered (180 min), its proteome largely returned to baseline, but key resistance-associated pathways, including the Rifampin ADP-ribosyltransferase superfamily and certain efflux systems, remained dysregulated. Parallel studies on Mtb H37Ra also demonstrated a distinct proteomic shift, comprising conserved adaptive responses such as ribosomal perturbation and compensatory transcriptional activity, as well as species-specific dysregulation of drug influx/efflux pumps and cell envelope remodelling via the polyketide synthase family. These findings demonstrate that sublethal rifampicin exposure primes mycobacteria for enhanced tolerance to lethal drug concentrations, underscoring a significant challenge in current TB therapy."],"pubmed_title":["Beyond Inhibition: Sublethal Rifampicin-Induced Molecular Adaptations Confer Phenotypic Drug Tolerance in Mycobacteria."],"pubmed_authors":["Stuart Blake D BD, van der Merwe Anja A, Chengalroyen Melissa D MD, Moosa Atica A, Warner Digby F DF, Blackburn Jonathan M JM, Ganief Tariq A TA"],"additional_accession":[]},"is_claimable":false,"name":"Beyond Inhibition - Sub-lethal Rifampicin-induced molecular adaptations confer phenotypic drug tolerance in Mycobacteria","description":"This study investigates how sub-lethal concentrations of rifampicin act as a signalling stimulus that induces phenotypic drug tolerance in mycobacteria. Using Mycobacterium smegmatis as a fast-growing model and validating findings in the attenuated Mycobacterium tuberculosis H37Ra strain, we examined growth dynamics and proteomic adaptations following exposure to half the minimum inhibitory concentration (MIC) of rifampicin. Growth assays demonstrated a transient growth deceleration followed by recovery, indicative of acquired phenotypic tolerance. Deep data-independent acquisition parallel accumulation–serial fragmentation (DIA-PASEF) proteomics was employed to capture early (45 min) and adaptive (180 min) molecular responses in M. smegmatis, with early validation in M. tuberculosis H37Ra. Proteomic analyses revealed rapid and dynamic remodelling of transcriptional, translational, metabolic, and stress-response pathways, including ribosomal perturbation, altered purine biosynthesis, activation of resistance-associated regulators, and modulation of predicted rifampicin influx and efflux systems. In M. tuberculosis H37Ra, conserved responses were accompanied by pronounced cell envelope remodelling via polyketide synthase pathways. Functional validation confirmed that sub-MIC rifampicin pre-exposure primes mycobacteria for enhanced tolerance to lethal drug concentrations. These findings highlight sub-lethal antibiotic exposure as a critical driver of phenotypic tolerance, with important implications for tuberculosis treatment efficacy and resistance evolution.","dates":{"publication":"2026-06-08","submission":"2026-03-19"},"accession":"PXD075873","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:184922","NEWT:6703","NEWT:3555","NEWT:71647","NEWT:2","NEWT:157546","NEWT:474186","NEWT:35554","NEWT:38942","NEWT:307972","NEWT:32046","NEWT:1496","NEWT:544496","NEWT:2102","NEWT:2042546","NEWT:259447","NEWT:45351","NEWT:445974","NEWT:43179","NEWT:4513","NEWT:180454","NEWT:5722","NEWT:1247","NEWT:6938","NEWT:1129","NEWT:55153","NCBITaxon:10407","NEWT:1736309","NEWT:309800","NEWT:281395","NEWT:10360","NEWT:1211601","NEWT:876138","NEWT:47664","NEWT:317548","NEWT:3654","NEWT:237561","NEWT:5833","NEWT:6928","NEWT:10036","NEWT:36745","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:2649997","NEWT:272563","NEWT:224326","NEWT:1333499","NCBITaxon:79857","NEWT:1096976","NEWT:82688","NEWT:95648","NEWT:3885","NEWT:3888","NEWT:5821","NEWT:1589","NEWT:135622","NCBITaxon:4896","NEWT:6915","NEWT:3649","NEWT:101510","NEWT:28903","NEWT:3880","NEWT:272559","NEWT:28909","NEWT:515849","NEWT:3641","NEWT:383379","NEWT:466585","NEWT:10029","NEWT:913645","NEWT:1000589","NEWT:85963","NEWT:85962","NEWT:143361","NEWT:317447","NEWT:4688","NEWT:7955","NEWT:7959","NEWT:2261","NEWT:31156","NEWT:398580","NEWT:4442","NEWT:4565","NEWT:1264690","NEWT:515619","NEWT:192875","NEWT:34305","NEWT:59729","NEWT:2164133","NCBITaxon:183674","NEWT:1316931","NEWT:224308","NEWT:167387","NEWT:84645","NEWT:44586","NEWT:626528","NEWT:3347","NEWT:511145","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:5888","NEWT:211586","NEWT:747078","NEWT:1282","NEWT:1283","NEWT:931281","NEWT:4550","NEWT:1000561","NEWT:2133","NEWT:294381","NEWT:197","NEWT:1390363","NEWT:77133","NEWT:288705","NEWT:29176","NEWT:145481","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:44689","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:556182","NEWT:1270","NEWT:374990","NEWT:1392","NEWT:498217","NEWT:156471","NEWT:2242","NEWT:4784","NEWT:11320","NEWT:360106","NEWT:156476","NEWT:286","NEWT:391619","NEWT:360104","NEWT:246196","NEWT:287","NEWT:246197","NEWT:10117","NEWT:10239","NEWT:10116","NEWT:1280","NEWT:1735272","NEWT:83334","NEWT:83332","NEWT:44685","NEWT:317513","NEWT:161934","NEWT:1148","NEWT:580240","NEWT:5508","NEWT:294128","NEWT:5507","NEWT:11676","NEWT:55571","NEWT:35500","NEWT:1140","NEWT:100226","NEWT:4530","NEWT:1143","NEWT:4896","NEWT:75058","NEWT:13616","NEWT:1390","NEWT:1094343","NEWT:1336795","NEWT:644042","NEWT:296543","NEWT:316435","NEWT:42157","NEWT:1773","NEWT:1895","NEWT:1182590","NEWT:3712","NEWT:82380","NEWT:1034304","NEWT:105023","NEWT:866628","NEWT:935293","NEWT:64152","NEWT:4924","NEWT:749200","NEWT:375146","NEWT:9378","NEWT:990346","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:263","NEWT:230741","NEWT:52283","NEWT:284812","NEWT:8175","NCBITaxon:1313","NEWT:43330","NEWT:1603293","NEWT:408169","NEWT:44544","NEWT:47946","NEWT:4911","NEWT:645463","NEWT:3702","NEWT:129249","NEWT:1077286","NEWT:243277","NEWT:990119","NEWT:2850","NEWT:1111708","NEWT:408172","NEWT:227321","NEWT:408170","NEWT:493760","NEWT:106590","NEWT:260710","NEWT:257313","NEWT:3827","NEWT:400772","NEWT:3708","NEWT:128161","NEWT:332648","NEWT:930945","NEWT:106592","NEWT:536231","NEWT:46704","NEWT:1436733","NEWT:460519","NEWT:1187947","NEWT:572307","NEWT:1432138","NEWT:269796","NEWT:10312","NEWT:1424507","NCBITaxon:1773","NEWT:1194599","NEWT:272844","NEWT:54571","NEWT:9598","NEWT:8030","NEWT:9483","NEWT:1513458","NCBITaxon:40559","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:67767","NEWT:759272","NEWT:432359","NEWT:46835","NEWT:1182263","NEWT:109757","NEWT:943146","NEWT:2711","NEWT:300852","NEWT:1502","NEWT:376686","NEWT:95486","NEWT:9103","NEWT:508771","NEWT:1883446","NEWT:29159","NEWT:253","NEWT:10306","NCBITaxon:2759","NEWT:1233435","NEWT:93061","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NEWT:240906","NEWT:593117","NEWT:89920","NEWT:29158","NEWT:3635","NEWT:5811","NEWT:235443","NEWT:180923","NEWT:108458","NEWT:272623","NEWT:272624","NEWT:411483","NEWT:884019","NEWT:198215","NEWT:411490","NEWT:983964","NEWT:118499","NEWT:169963","NEWT:32644","NEWT:527796","NEWT:225117","NEWT:746128","NEWT:499175","NEWT:109779","NEWT:43665","NEWT:1266464","NEWT:1715989","NEWT:476272","NEWT:3747","NEWT:195051","NEWT:367830","NEWT:1255228","NEWT:178616","NEWT:649908","NEWT:410289","NEWT:373153","NEWT:375451","NEWT:352472","NEWT:357","NEWT:1071661","NEWT:360094","NEWT:470","NEWT:41364","NEWT:1313","NEWT:411469","NEWT:84023","NEWT:1679","NEWT:559292","NEWT:39491","NCBITaxon:5811","NEWT:29491","NEWT:411464","NEWT:411460","NEWT:2014887","NEWT:2762","NEWT:1174673","NEWT:1328426","NEWT:562","NEWT:411470","NEWT:33952","NEWT:2094720","NCBITaxon:2697049","NEWT:571256","NCBITaxon:138","NEWT:40483","NEWT:28038","NEWT:1663","NEWT:1423","NEWT:4932","NEWT:3603","NEWT:2759","NEWT:3847","NEWT:38293","NEWT:1428","NEWT:327159","NEWT:178876","NEWT:1660","NEWT:327160","NEWT:573","NEWT:9031","NEWT:1872122","NEWT:7091","NEWT:108931","NEWT:241368","NEWT:1055524","NEWT:42528","NEWT:190802","NEWT:9778","NEWT:150475","NEWT:303","NEWT:9417","NEWT:7111","NEWT:347515","NEWT:1216979","NEWT:7237","NEWT:9534","NEWT:5180","NEWT:256737","NEWT:9541","NEWT:115104","NEWT:1121114","NEWT:663","NEWT:1081927","NEWT:1238993","NEWT:67825","NEWT:187878","NEWT:185579","NEWT:941442","NEWT:220668","NEWT:13076","NEWT:1821314","NEWT:1249668","NEWT:7108","NEWT:40479","NEWT:317","NEWT:5286","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:9402","NEWT:9644","NEWT:415540","NEWT:550","NEWT:675060","NEWT:4081","NEWT:334542","NEWT:273068","NEWT:554","NEWT:27592","NEWT:98334","NEWT:426428","NEWT:451516","NEWT:63459","NEWT:1276815","NEWT:36185","NEWT:588858","NEWT:1225786","NEWT:9639","NCBITaxon:11071","NEWT:242231","NEWT:7574","NEWT:1715256","NEWT:197221","NEWT:7215","NEWT:575412","NEWT:929793","NEWT:29204","NEWT:28112","NEWT:2172103","NEWT:6494","NEWT:7460","NEWT:6491","NEWT:507601","NEWT:643680","NCBITaxon:6157","NEWT:42752","NEWT:746360","NEWT:6239","NEWT:89764","NEWT:470150","NEWT:162425","NEWT:216257","NEWT:102169","NEWT:543734","NEWT:9986","NEWT:4054","NEWT:73239","NEWT:226186","NEWT:1268063","NEWT:8782","NEWT:1263854","NEWT:118503","NEWT:2059687","NEWT:115357","NEWT:435590","NEWT:1902","NEWT:160488","NEWT:28104","NEWT:1908","NEWT:13164","NEWT:216129","NCBITaxon:2","NEWT:985076","NEWT:1215323","NEWT:2664179","NEWT:986","NEWT:52641","NEWT:7038","NEWT:7159","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:353152","NEWT:2829","NEWT:366581","NEWT:216599","NEWT:216595","NEWT:1194669","NEWT:51329","NEWT:40559","NEWT:243230","NEWT:8355","NEWT:633","NEWT:9685","NEWT:7029","NEWT:1080772","NEWT:8479","NEWT:1283300","NEWT:186990","NEWT:632","NEWT:6183","NEWT:6063","NEWT:630","NEWT:49240","NEWT:334747","NEWT:61235","NEWT:15368","NEWT:6289","NEWT:436486","NEWT:6287","NEWT:300641","NEWT:727","NEWT:9796","NEWT:725","NEWT:170187","NEWT:469008","NEWT:260707","NEWT:256318","NEWT:206411","NCBITaxon:6191","NEWT:596153","NEWT:1836","NEWT:185431","NEWT:29760","NEWT:260704","NEWT:1719","NEWT:703612","NEWT:260705","NEWT:80863","NEWT:2697049","NEWT:105231","NEWT:1216981","NEWT:52638","NEWT:4097","NCBITaxon:6073","NEWT:884204","NEWT:9785","NEWT:6279","NEWT:1123869","NEWT:9544","NEWT:1329349","NEWT:7370","NEWT:83906","NEWT:607699","NCBITaxon:780","NCBITaxon:1502","NEWT:6282","NEWT:208963","NEWT:208964","NEWT:27337","NEWT:1134506","NEWT:575584","NEWT:38783","NEWT:8727","NEWT:4006","NEWT:8726","NEWT:6426","NEWT:6669","NEWT:10090","NEWT:9935","NEWT:89453","NEWT:4120","NEWT:51515","NEWT:5693","NEWT:2186","NEWT:8724","NEWT:51511","NEWT:8845","NEWT:92867","NEWT:8723","NEWT:92866","NEWT:5334","NEWT:37334","NEWT:51750","NEWT:382352","NCBITaxon:10359","NEWT:1203258","NEWT:242619","NEWT:160791","NEWT:632957","NEWT:34073","NEWT:373995","NEWT:5689","NEWT:544404","NEWT:9925","NEWT:89462","NEWT:8839","NEWT:4232","NEWT:2758385","NEWT:37349","NEWT:4113","NEWT:837","NEWT:11298","NEWT:171101","NEWT:714","NEWT:36015","NEWT:421932","NEWT:312017","NEWT:196627","NEWT:5691","NEWT:48479","NEWT:627025","NEWT:1097677","NEWT:375939","NEWT:61674","NEWT:870435","NEWT:1117957","NEWT:6526","NEWT:9913","NEWT:8709","NEWT:105841","NEWT:337330","NEWT:4100","NEWT:2666255","NEWT:1159556","NEWT:5671","NEWT:2285","NEWT:1076","NEWT:4101","NEWT:6763","NEWT:803","NEWT:29722","NEWT:380394","NEWT:1692259","NEWT:1486917","NEWT:981087","NEWT:180066","NEWT:226900","NEWT:468911","NEWT:817","NEWT:7604","NEWT:818","NEWT:135588","NEWT:1843183","NEWT:5661","NEWT:29719","NEWT:58002","NEWT:326423","NEWT:452467","NEWT:36111","NEWT:326424","NEWT:4577","NEWT:1416333","NEWT:5664","NEWT:2157","NEWT:1678078","NEWT:749906","NEWT:418985","NEWT:146479","NEWT:749907","NEWT:1042986","NEWT:1911079","NEWT:69373","NEWT:145263","NEWT:264203","NEWT:1480154","NEWT:694569","NEWT:1274414","NEWT:27606","NEWT:9739","NEWT:59202","NEWT:9975","NEWT:9612","NEWT:38626","NEWT:38865","NEWT:51953","NEWT:3077","NEWT:3197","NEWT:9615","NEWT:10299","NEWT:860688","NEWT:112273","NEWT:36329","NEWT:1147787","NEWT:62615","NCBITaxon:3044782","NEWT:1440772","NEWT:72407","NEWT:1355477","NEWT:349741","NEWT:9605","NEWT:9606","NEWT:90371","NEWT:157295","NEWT:641501","NEWT:4153","NEWT:2096","NEWT:7668","NEWT:915099","NEWT:74940","NEWT:9721","NEWT:137221","NEWT:9600","NEWT:9963","NEWT:1450511","NEWT:1143193","NEWT:1617910","NEWT:174934","NEWT:565050","NEWT:2823525","NEWT:2785785","NEWT:411901","NEWT:9838","NEWT:105884","NCBITaxon:9615","NEWT:9839","NEWT:58334","NEWT:1193501","NEWT:3055","NEWT:58331","NEWT:4146","NEWT:5478","NEWT:6326","NEWT:6689","NEWT:5476","NEWT:1450520","NEWT:299767","NEWT:28835","NEWT:1274432","NEWT:2053667","NEWT:1274426","NEWT:70448","NEWT:9825","NEWT:1274423","NEWT:393765","NEWT:698936","NEWT:39946","NEWT:9823","NEWT:9940","NEWT:95602","NEWT:8732","NEWT:521001","NEWT:192011","NEWT:1813887","NEWT:39947","NEWT:224911","NEWT:1274420","NEWT:578458"],"pubmed":["42048180"],"ORCID":["0009-0003-2537-2046"]}}