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Facility"],"technology_type":["Mass Spectrometry","Top-down proteomics"],"software":[""],"submitter_keywords":["Multiple sclerosis","Proteomics","Serum"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD077759"],"tissue":["Blood Serum"],"sample_protocol":["We extracted proteins following the method described earlier (18). Briefly, 5 µL of serum from each sample was aliquoted to an Eppendorf tube, and 300 µL of MeOH (Fisher Scientific, Loughborough, UK) was subsequently mixed with the aliquot. Following vortexing, the samples were refrigerated at −20 °C for 2 hours and then centrifuged at 14,000 rpm for 15 minutes at 24 °C to precipitate the protein. After air-drying, the protein extracts were resuspended in 100 µL of a buffer consisting of 6M urea and 2M thiourea in a 10 mM Tris at pH 8. Proteins were subsequently estimated using the modified Bradford (Sigma, St Louis, MO, USA) (19). 2.5 Enzymatic Digestion and Peptide Desalting Protein samples in the amount of 100 µg were reduced with 1 mM dithiothreitol (DTT) (Thermo Scientific, Rockford, USA) and subsequently incubated at room temperature (RT) for 1 hour with modest mixing. Following this, the samples were alkylated by adding 5.5 mM iodoacetamide (IAA) (Thermo Scientific, Rockford, USA) and maintained at room temperature in the dark for 1 hour. pH measurements were conducted at the end of each stage, and if necessary, pH levels were adjusted to 8.0. Following this, 1 µg of lysyl-endopeptidase LysC (1:100, w/w) was introduced to the samples, and they were maintained at room temperature for three hours at 100 rpm. After being diluted 4 times with 20 mM ammonium bicarbonate, the samples were digested with 1 µg trypsin (1:100 ratio) and maintained by gently mixing for approximately 18 hours at RT. The reaction was subsequently halted by acidification to 1% trifluoroacetic acid (TFA) (Fisher Scientific, Loughborough, UK). Every sample underwent drying during the desalting process, and they were subsequently reconstituted in 1% TFA and filtered with C18 STAGE (Stop And Go Extraction) tips. 100 µL of 50% acetonitrile (ACN) (Wunstorfer Strasse, Seelze, Germany) was aspirated and dispensed to condition C18 membranes, and 100 µL of 0.1% TFA was added to equilibrate the sample. By aspirating and loading the samples 10x, the protein samples were loaded. Subsequently, the columns were washed with 0.1% TFA in 5% ACN. Following this, the peptides were eluted into LC containers through aspiration and the dispensation of 100 µL of 0.1% formic acid (FA) (Fisher Scientific, Loughborough, UK) in 60% ACN. Prior to LC-MS/MS analysis, the eluted peptide samples were reconstituted in 100 µL of 0.1% FA in 2% ACN after being dehydrated using EZ-2 Plus (GeneVac, Ipswich, UK)."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["To detect the peptides and proteins, the raw data were analyzed by MaxQuant version 2.0.3.0. utilizing the UniProt proteome for Homo sapiens and the Andromeda search engine (https://www.uniprot.org/proteomes/UP000005640). The MS/MS database search was conducted using standard settings, which included the carbamidomethylation of cysteine residues as a fixed modification and methionine oxidation and acetylation of protein N-termini as variable modifications. A 20-ppm precursor mass tolerance and a 1% false discovery rate (FDR) were utilized to filter the peptide spectral matching (PSM). The MaxLFQ method was used to quantify label-free data (LFQ). The default trypsin/P enzymatic cleavage protocol was employed in the in-silico digestion. In order to guarantee a valid label-free quantification, the protein groups were detected by assigning at ≥ 2 peptides, ≥1 of which was unique to each protein. Using the t-test and one-way ANOVA with GraphPad Prism 9 (GraphPad Software, San Diego, CA), a p-value of <0.05 was regarded as statistically significant. The receiver operating characteristic curves (ROC) were generated in biomarker module of Metaboanalyst 6.0 (Montreal, QC, Canada) (http://www.metaboanalyst.ca)."],"omics_type":["Proteomics"],"labhead":["Prof. Mohammad Harb Semreen"],"instrument_platform":[""],"submission_type":["PARTIAL"],"labhead_affiliation":["1. Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates. 2. College of Pharmacy, Department of Medicinal Chemistry, University of Sharjah, 27272, United Arab Emirates"],"species":["Homo Sapiens (human)"],"publication":["Not available"],"submitter_mail":["tims-tof@sharjah.ac.ae"],"submitter_affiliation":["Sharjah Institute for Medical Research, University of Sharjah"],"submitter_country":["United Arab Emirates"],"additional_accession":[]},"is_claimable":false,"name":"Serum Proteomics Analysis Identified Junction Plakoglobin and Glyceraldehyde-3-Phosphate Dehydrogenase as Potential Novel Circulating Biomarkers for Patients with Multiple Sclerosis","description":"Multiple Sclerosis (MS) is a multifaceted neurological condition associated with recurrent inflammatory episodes, demyelination, axonal injury, and resultant functional impairment. MS has a diverse array of clinical manifestations, with the progressive variants culminating in irreparable neurological impairment. Sensitive and accurate biomarkers are required to diagnose, predict disease progression, and tailor therapy methods for MS. Here, we utilized liquid chromatography mass spectrometry-based Proteomics (LC-MS/MS) to find biomarkers in serum from patients with MS. The study examined variations of protein expression in healthy (n = 20) and MS groups (n = 60), comprising 20 patients in each disease course (relapsing-remitting (RRMS), primary progressive (PPMS), and secondary progressive MS (SPMS)). The enzyme-linked immune assay (ELISA) was then used to verify a candidate protein on serum samples obtained from patients with MS (n = 15) and healthy controls (HC) (n = 18) (validation cohort). Our findings showed 12 differentially expressed proteins (DEPs). Among the discovered proteins, some have been previously documented in patients with MS and animal models, while others constitute novel findings. We found two proteins, junction plakoglobin protein (JUP) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), that were downregulated and upregulated in patients with MS in comparison with HCs, respectively. The validation results of these two proteins by ELISA were consistent with those from LC-MS/MS. The identification of these proteins may assist in the elucidation of pathogenetic processes and might open the door to novel methods of therapy for individuals with MS.","dates":{"publication":"2026-04-29","submission":"2026-04-28"},"accession":"PXD077759","cross_references":{"TAXONOMY":["NEWT:3555","NEWT:71647","NEWT:330879","NEWT:260201","NEWT:35554","NCBITaxon:12939","NEWT:32046","NEWT:544496","NEWT:59529","NEWT:2042546","NEWT:32049","NEWT:1392696","NEWT:259447","NEWT:295546","NEWT:45351","NEWT:445974","NEWT:43179","NEWT:180454","NEWT:5722","NCBITaxon:1280","NEWT:112503","NEWT:1129","NEWT:309807","NEWT:10245","NEWT:55153","NEWT:89184","NEWT:309800","NEWT:281395","NEWT:1211601","NEWT:876138","NEWT:44271","NEWT:193516","NEWT:351581","NEWT:43186","NEWT:428146","NEWT:1117","NEWT:498257","NEWT:10036","NEWT:1590","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:2649997","NEWT:1147161","NEWT:661410","NEWT:638632","NEWT:263737","NEWT:224326","NEWT:1333499","NEWT:376619","NCBITaxon:79857","NEWT:1096976","NEWT:5702","NEWT:95648","NEWT:1589","NEWT:135622","NEWT:1349","NEWT:35786","NEWT:1580","NEWT:399784","NEWT:96731","NEWT:383379","NEWT:1401257","NEWT:418106","NEWT:10029","NEWT:913645","NEWT:44491","NEWT:641809","NEWT:27933","NEWT:556484","NEWT:317447","NEWT:4688","NEWT:7955","NEWT:7719","NEWT:7959","NEWT:2261","NEWT:868565","NEWT:135674","NEWT:94323","NEWT:31156","NEWT:3112","NEWT:79329","NEWT:4442","NEWT:192875","NEWT:30069","NEWT:12637","NEWT:59729","NEWT:2164133","NEWT:284218","NEWT:295105","NEWT:108061","NEWT:60711","NEWT:1316931","NEWT:224308","NEWT:3347","NEWT:511145","NEWT:160621","NEWT:212790","NEWT:931281","NEWT:4432","NEWT:5762","NEWT:5763","NEWT:658457","NEWT:1310161","NEWT:77133","NEWT:295358","NEWT:145481","NEWT:1310165","NEWT:29058","NCBITaxon:79824","NEWT:2246","NEWT:2652724","NEWT:5757","NEWT:68415","NCBITaxon:4563","NEWT:5755","NEWT:44688","NEWT:44689","NEWT:44447","NEWT:752555","NEWT:498211","NEWT:5759","NEWT:347256","NEWT:1736231","NEWT:5518","NEWT:398007","NEWT:1527468","NEWT:1392","NEWT:498217","NEWT:498216","NEWT:2242","NEWT:11320","NEWT:286","NEWT:391619","NEWT:246196","NEWT:287","NEWT:246197","NEWT:145458","NEWT:633149","NEWT:1149786","NEWT:10239","NEWT:68895","NEWT:44685","NEWT:161934","NEWT:4897","NEWT:1148","NEWT:5744","NEWT:72901","NEWT:213863","NEWT:5508","NEWT:3329","NEWT:5507","NEWT:410661","NEWT:55571","NEWT:35500","NEWT:1140","NCBITaxon:2157","NEWT:1143","NEWT:4896","NEWT:1287689","NEWT:1390","NEWT:11557","NEWT:1094343","NEWT:1462472","NEWT:1336795","NEWT:644042","NEWT:294","NEWT:1773","NEWT:1182590","NEWT:3712","NEWT:82380","NEWT:3711","NEWT:935293","NEWT:375146","NEWT:2065263","NEWT:177437","NEWT:1772","NEWT:10418","NEWT:118698","NEWT:1616117","NEWT:263","NEWT:118696","NEWT:34865","NEWT:52283","NEWT:11988","NEWT:284812","NEWT:8175","NEWT:400667","NEWT:43330","NEWT:1603293","NEWT:408169","NEWT:44664","NEWT:47946","NEWT:3702","NEWT:1245466","NEWT:243277","NEWT:1246791","NEWT:7067","NEWT:118694","NEWT:586722","NEWT:2850","NEWT:118691","NEWT:34871","NEWT:33548","NEWT:274","NEWT:7070","NCBITaxon:287","NEWT:408172","NEWT:408170","NEWT:96794","NEWT:3708","NEWT:332648","NEWT:44670","NEWT:536231","NEWT:376219","NEWT:219813","NEWT:1510","NEWT:1436733","NEWT:460519","NEWT:1247411","NEWT:1515","NEWT:572307","NEWT:1432138","NEWT:1424507","NEWT:1194599","NEWT:272844","NEWT:1303443","NEWT:333760","NEWT:34607","NEWT:56636","NEWT:1513458","NEWT:1233681","NCBITaxon:40559","NEWT:506599","NEWT:2853422","NEWT:81077","NEWT:84588","NEWT:1679718","NEWT:480","NEWT:65349","NEWT:67767","NEWT:46835","NEWT:109757","NEWT:582580","NEWT:300852","NEWT:1502","NEWT:128017","NEWT:376686","NEWT:95486","NEWT:508771","NEWT:1883446","NEWT:253","NCBITaxon:2759","NEWT:1233435","NEWT:93061","NEWT:93062","NEWT:34613","NCBITaxon:4932","NEWT:109760","NEWT:29031","NEWT:943274","NEWT:644223","NEWT:235443","NEWT:108458","NEWT:5936","NEWT:272623","NEWT:272624","NEWT:320637","NEWT:886559","NEWT:3750","NEWT:55521","NEWT:983964","NEWT:118499","NEWT:11706","NEWT:32644","NEWT:527796","NEWT:1130798","NEWT:55529","NEWT:499175","NEWT:109779","NEWT:3745","NEWT:1715989","NCBITaxon:4751","NEWT:476272","NEWT:3747","NEWT:195051","NEWT:3988","NEWT:1116234","NEWT:561007","NEWT:1255228","NEWT:649908","NEWT:410289","NEWT:373153","NEWT:3983","NEWT:203696","NEWT:472","NEWT:352472","NEWT:97441","NEWT:1071661","NEWT:1202531","NEWT:1202532","NEWT:360094","NEWT:1408223","NEWT:470","NEWT:1408224","NEWT:41364","NEWT:5911","NEWT:1313","NEWT:1218097","NEWT:1080348","NCBITaxon:50557","NEWT:1314","NEWT:39251","NEWT:39491","NEWT:212142","NCBITaxon:5811","NEWT:29491","NEWT:101841","NEWT:153481","NEWT:2014887","NEWT:33952","NEWT:445","NEWT:153009","NEWT:261756","NEWT:571256","NEWT:40483","NEWT:63366","NEWT:63367","NEWT:688333","NEWT:215402","NCBITaxon:548681","NEWT:318586","NCBITaxon:5820","NEWT:1547","NEWT:272634","NEWT:198107","NEWT:9031","NEWT:27292","NEWT:1872122","NEWT:1308","NEWT:7091","NEWT:1034015","NEWT:760568","NEWT:141262","NEWT:108931","NEWT:1293497","NEWT:1055524","NEWT:4087","NEWT:9778","NEWT:306","NEWT:150475","NEWT:303","NEWT:267872","NEWT:97477","NEWT:7111","NEWT:347515","NEWT:172269","NEWT:953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