Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211220_WT1_PM_QO_i03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211220_WT1_PM_QO_i02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211220_WT1_PM_QO_i04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211221_WT1_INC1_i02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211220_WT1_PM_QO_i05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211221_WT1_INC1_i01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211221_WT1_SN_i01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211221_WT1_SN_i02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211220_WT1_PM_QO_i01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211221_WT1_INC2_i02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20211221_WT1_INC2_i01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/TriTrypDB-68_TbruceiTREU927_AnnotatedProteins_contam.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/TriTrypDB-42_TbruceiLister427_AnnotatedProteins_v2.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_FeNTa_7_DDA_CW_i01_Slot2-13_1_7766.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC1_3_DIA_CW_i01_Slot2-11_1_7759.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_1_0_04L_0_4H_DDA_CW_i01_Slot2-10_1_7797.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_5_5L_0_4H_DDA_CW_i01_Slot2-13_1_7806.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_8_0_4L_5H_DDA_CW_i01_Slot2-15_1_7812.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_FeNTa_7_DIA_CW_i01_Slot2-13_1_7767.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC2_7_DIA_CW_i01_Slot2-14_1_7771.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_6_5L_0_4H_DDA_CW_i01_Slot2-14_1_7809.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/FragPipe_Proteome.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/MaxQuant_PM_PHOS.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC1_7_DDA_CW_i01_Slot2-15_1_7772.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC1_3_DDA_CW_i01_Slot2-11_1_7758.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_FeNTa_7_DDA_CW_i01_Slot2-17_1_7779.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC2_7_DDA_CW_i01_Slot2-14_1_7770.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/MaxQuant_PM.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC1_7_DIA_CW_i01_Slot2-15_1_7773.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_1_SN_0_04L_0_4H_DDA_CW_i01_Slot2-19_1_7816.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_TiO2_3_DDA_CW_i01_Slot2-19_1_7785.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_5_SN_5L_0_4H_DDA_CW_i01_Slot2-22_1_7825.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_FeNTa_3_DDA_CW_i01_Slot2-18_1_7782.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_FeNTa_7_DIA_CW_i01_Slot2-17_1_7780.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_TiO2_3_DIA_CW_i01_Slot2-19_1_7786.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_4_SN_0_4L_0_04H_DDA_CW_i01_Slot2-21_1_7822.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/FragPipe_Phos.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_TiO2_7_DDA_CW_i01_Slot2-16_1_7776.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_FeNTa_3_DIA_CW_i01_Slot2-18_1_7783.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_SN_TiO2_7_DIA_CW_i01_Slot2-16_1_7777.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20251023_FeNTa_3_DIA_CW_i01_Slot2-12_1_7763.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_2_0_04L_0_4H_DDA_CW_i01_Slot2-11_1_7800.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC2_3_DIA_CW_i01_Slot2-10_1_7757.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20251023_FeNTa_3_DDA_CW_i01_Slot2-12_1_7762.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_6_SN_5L_0_4H_DDA_CW_i01_Slot2-23_1_7828.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260203_TiO2_INC2_3_DDA_CW_i01_Slot2-10_1_7756.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_4_0_4L_0_04H_DDA_CW_i01_Slot2-12_1_7803.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_2_SN_0_04L_0_4H_DDA_CW_i01_Slot2-20_1_7819.d.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079824/20260210_8_SN_0_4L_5H_DDA_CW_i01_Slot2-24_1_7813.d.zipprimaryOK200doris.rentsch@unibe.chManfred HellerMass SpectrometryBottom-up proteomicsTb927.8.8300Branched-chain amino acidsAmino acidPhosphoproteomicsSubstrate specificityParasiteTransporthttps://www.ebi.ac.uk/pride/archive/projects/PXD079824Phosphoproteome: T. brucei cells (strain 29-13), equivalent to 1 mg of total proteins, as determined by Bradford (1), were washed in PBS and frozen at -80°C. Samples were processed by the Proteomics & Mass Spectrometry Core Facility of the department for BioMedical Research of the University of Bern according to their validated protocols for phospho-proteome analyses (2), and (plasma-)membrane protein enrichment using a two-step ultracentrifugation protocol (3). Phosphorylation comparison in different BCAA concentrations using SILAC: T. brucei cells (strain 29-13) were washed in PBS and resuspended in SDM-80 (4) containing 5.55 mM glucose, 10% dialyzed FCS (BioConcept, Switzerland) and either light (12C6/14Nχ) or heavy (13C6/15Nχ) isotopes of arginine (1.1 mM) and lysine (0.4 mM) (Euroisotope), as well as 0.4 mM BCAA. The cells were cultivated for six days. On day six, the cells were washed twice in SDM-80 containing either 0.04 mM BCAA, 0.4 mM BCAA or 5 mM BCAA and diluted in SDM-80 containing the corresponding BCAA concentration. After 24 h, for the first analysis, 0.75·108 cells grown in 0.04 mM BCAA were mixed with 0.75·108 cells grown in 0.4 mM BCAA. For the second analysis, 0.75·108 cells grown in 0.4 mM BCAA were mixed with 0.75·108 cells grown in 5 mM BCAA. The cells were washed in PBS and the cell pellets flash frozen in liquid nitrogen. Each cell pellet contained the equivalent of 1.1 mg of total protein, as determined by Bradford (1). Four biological replicates, including a label switch were prepared for the 0.04/0.4 mM BCAA and 0.4/5 mM BCAA analyses. Frozen cell pellets were homogenized in 500 µL 8M urea in 100mM Tris-HCl pH 8.00 containing a protease (Complete EDTA free, Roche, Switzerland) and phosphatase inhibitor cocktail (10mM NaF/2mM Na2VO4) by probe sonication on ice with six bursts of 10 seconds and intermittent cooling on ice. The supernatant after centrifugation of the lysate for 4 minutes at 10’000 g and 4°C was transferred into a new vial. Protein concentration was determined with Bradford assay and proteins reduced and alkylated with 10 mM DTT (30 minutes) followed by 50 mM iodoacetamide for 30 minutes at 37°C in the dark. Proteins were precipitated overnight with 5 volumes of acetone at -20°C. Proteins were pelleted by centrifugation for 10 minutes at 4°C and 16’000 g, liquid supernatant carefully removed, and the protein pellet dried for 15 minutes at ambient air. Proteins were reconstituted with 100 µL 8M urea/50mM Tris/HCl pH 8.00 and protein content determined again with Bradford assay. An aliquote corresponding to 1 mg protein was diluted five times with 20mM Tris/HCl pH 8.00, 2 mM CaCl2 and digested with porcine trypsin (Sigma, Switzerland) at an enzyme-to-protein ratio of 1:50 over night at ambient temperature. Digestion was stopped by acidifying with 1/20 volume of 20% TFA. The supernatant after centrifugation for 1 minute at 20°C and 16’000 g was then cleaned through C18 cartridges (SEP Pak 1cc, Waters, Switzerland) according to manufacturer’s recommendations. Eluates were dried under nitrogen, and peptides resuspended in 110 µL 80% ACN, 0.1% TFA. Phosphopeptide enrichment was carried out on an AssayMAP Bravo (Agilent Technologies) platform using the Phospho Enrichment v2.0 protocol and the 5 µL Fe (III)-NTA cartridges. Briefly the cartridges were primed with 100 µL 50% ACN, 0.1% TFA at a flow rate of 300 µL/min and equilibrated with 50 µL loading buffer (80% ACN, 0.1% TFA) at 10 µL/min. Samples were loaded onto the cartridge at 3.5 µL/min. The cartridges were washed with 50μL loading buffer and the phosphorylated peptides were eluted with 20 μL 1% ammonia directly into 2 μL 100% formic acid and further acidified with 2.5 µL TFA 20%. The samples were analysed by nano-liquid chromatography on a nanoElute 2 UPLC coupled to a timsTOF HT (Bruker Daltonics, Bremen, Germany), through a CaptiveSpray source (Bruker, Bremen, Germany) with an end-plate offset of 500 V, a drying temperature of 200 °C, and with the capillary voltage fixed at 1.6 kV. A volume of 4 µL from the enriched peptides were loaded directly onto the analytical column (PepSepC18 1.5 Å, 150 μm × 15 cm, Bruker) with 0.05% TFA in water/acetonitrile 98:2 with a fixed pressure of 800 bar. After loading, peptides were eluted with a 30 min active peptide separation gradient and electrosprayed into the mass spectrometer. Data were acquired in DDA method employing eight PASEF ramps with an ion mobility range from 0.75 to 1.35 1/k0, in the m/z range between 100–1700 u and a cycle time of 0.95 s at an acquisition rate of 300Hz. For DIA 32 isolation windows were set, each 26 m/z wide, with a 1 m/z overlap between adjacent windows. These isolation windows corresponded to an ion mobility range of 0.75–1.36 V s/cm². Both TIMS accumulation and separation times were set at 100 ms. The same analysis was carried out with 4 µL of the flow-through from the FeNTA cartridges what we called “total proteome”.PrideNot availablePhosphoproteome: Processed as published earlier (2) using MaxQuant (version 1.6.14.0) against the TriTrypDB-50_TbruceiTREU927 protein sequence database, including some common contaminants. Results are from a single phosphoproteome- and a single (plasma)membrane proteome analysis. Phosphorylation comparison in different BCAA concentrations using SILAC: The mass spectrometry data were interpreted with FragPipe (5) against the TriTrypDB-68_TbruceiTREU927 protein sequences, including some common contaminants. Search parameters included precursor and fragment mass tolerances of 20 ppm and 0.05 Da; strict trypsin cleavage rule without cuts if proline follows arginine or lysine and allowing two missed cleavages; fixed carbamidomethylation modification on cysteines and oxidation of methionine (maximum 1 time per peptide), protein N-terminal acetylation, phosphorylation of serine/threonine/tyrosine (max. 3x), and heavy label of lysine (+8) and arginine (+10) as variable modifications. Peptide spectrum matches were re-scored with MS-Booster using the Prosit_2019_irt, Prosit_2023_intensity_timsTOF, and DIA-NN models for retention time, fragment spectrum and ion mobility verification followed by percolator for false discovery estimation. Peptide intensities and heavy-to-light ratios were calculated with the ionquant algorithm. For the total proteome data, identical search parameters were used with the exception that variable phosphorylation (2x) was only allowed on serine and threonine. 1. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254 2. Hallal, M., Braga-Lagache, S., Jankovic, J., Simillion, C., Bruggmann, R., Uldry, A.-C., Allam, R., Heller, M., and Bonadies, N. (2021) Inference of kinase-signaling networks in human myeloid cell line models by phosphoproteomics using kinase activity enrichment analysis (KAEA). BMC Cancer 21, 789-789 3. Timpanaro, A., Piccand, C., Uldry, A.-C., Bode, P. K., Dzhumashev, D., Sala, R., Heller, M., Rössler, J., and Bernasconi, M. (2023) Surfaceome profiling of cell lines and patient-derived xenografts confirm FGFR4, NCAM1, CD276, and highlight AGRL2, JAM3, and L1CAM as surface targets for rhabdomyosarcoma. International Journal of Molecular Sciences 24, 2601-2601 4. Lamour N, Riviere L, Coustou V, Coombs GH, Barrett MP & Bringaud F (2005) Proline metabolism in procyclic Trypanosoma brucei is down-regulated in the presence of glucose. J Biol Chem 280, 11902–11910. 5. Kong, Andy T, Felipe V Leprevost, Dmitry M Avtonomov, Dattatreya Mellacheruvu, and Alexey I Nesvizhskii. 2017. “MSFragger: Ultrafast and Comprehensive Peptide Identification in Mass Spectrometry-Based Proteomics.” Nat Methods (United States) 14 (5): 513–20. 43ProteomicsDoris RentschInstitute of Plant Sciences, University of Bern, Bern, SwitzerlandPARTIALTrypanosoma Brucei42520931 Oliveres QF, Haindrich AC, Piller S, Macedo JP, van Berkum V, Rogé Ferreira FL, Wenger C, Naguleswaran A, Rentsch D. The branched-chain amino acid transporter AAT10.2 of Trypanosoma brucei is regulated by substrate availability. J Biol Chem. 2026 302(9):113379 10.1016/j.jbc.2026.113379pmscf.dbmr@unibe.chProteomics and Mass Spectrometry Core Facility, Departement for Biomedical Research, University of BerneSwitzerlandAmino acid transporters (AATs) are essential for the uptake of exogenous amino acids, and their regulation allows the protozoan parasite Trypanosoma brucei to rapidly adapt to changing environmental conditions when alternating between the mammalian and the insect host, or when invading different host tissues. With a phosphoproteome analysis of procyclic form T. brucei we identified many AATs of the amino acid/auxin permease (AAAP) family with phosphorylated residues exclusively in their hydrophilic N-terminal domain. AAT10.2, which contained multiple phosphorylated residues, was further characterized and shown to be localized at the plasma membrane and the flagellar pocket. RNAi in procyclic form T. brucei revealed that AAT10.2 is essential and the main transporter for branched-chain amino acids (BCAA) under the conditions tested. High affinity and selective transport of BCAA was verified by expressing AAT10.2 in Saccharomyces cerevisiae. In T. brucei, AAT10.2 protein abundance was regulated by BCAA levels. However, mutation of the identified phosphorylation sites or truncation of the first 35 amino acids did not affect affinity, localization, or the BCAA-dependent regulation of AAT10.2. The non-phosphorylatable 3xTy-STA-AAT10.2∗ variant, in which N-terminal serine and threonine residues were mutated to alanine, showed increased protein levels compared to native Ty-tagged AAT10.2∗ or the phosphomimicking variant (3xTy-STD-AAT10.2∗) with aspartate replacing serine and threonine in the N-terminal region. Truncation of the N-terminal domain reduced the overall AAT10.2 abundance and BCAA levels in short-term experiments, and decreased T. brucei growth when BCAA availability was limited. These findings establish AAT10.2 as an essential, substrate-regulated BCAA transporter in procyclic form T. brucei.The branched-chain amino acid transporter AAT10.2 of Trypanosoma brucei is regulated by substrate availability.Oliveres Quentin-Florian QF, Haindrich Alexander C AC, Piller Silvan S, Macedo Juan P JP, van Berkum Veerle V, Rogé Ferreira Fabio Luiz FL, Wenger Christoph C, Naguleswaran Arunasalam A, Rentsch Doris DfalseThe branched-chain amino acid transporter AAT10.2 of Trypanosoma brucei is regulated by substrate availabilityA phosphoproteome analysis of procyclic form T. brucei revealed many amino acid transporters (AATs) of the amino acid/auxin permease (AAAP) family with phosphorylated residues exclusively in their hydrophilic N-terminal domain. AAT10.2, which contained multiple phosphorylated residues, was further characterized and shown to be localized at the plasma membrane and the flagellar pocket. RNAi in procyclic form T. brucei revealed that AAT10.2 is essential and the main transporter for branched-chain amino acids (BCAA). Furthermore, the abundance of AAT10.2 protein is regulated by BCAA levels. However, mutation of the identified phosphorylation-sites or truncation of the first 35 amino acids did not affect affinity, localization, or the BCAA-dependent regulation of AAT10.2. The non-phosphorylatable 3xTy-STA-AAT10.2* variant, in which N-terminal serine and threonine residues were mutated to alanine, showed increased protein levels compared to native Ty-tagged AAT10.2* or the phosphomimicking variant (3xTy-STD-AAT10.2*) with aspartate replacing serine and threonine in the N-terminal region. Truncation of the N-terminal domain reduced the overall AAT10.2 abundance and internal BCAA levels in short-term experiments, and decreased T. brucei growth when BCAA availability was limited. These findings establish AAT10.2 as essential, substrate-regulated BCAA transporter in procyclic form T. brucei.2026-09-142026-06-17PXD079824NEWT:3555NEWT:71647NEWT:330879NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:295546NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:309807NEWT:10245NEWT:55153NEWT:10244NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:43186NEWT:498257NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:661410NEWT:638632NEWT:263737NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:5702NEWT:95648NEWT:1589NEWT:135622NEWT:1349NEWT:67352NEWT:35786NEWT:1580NEWT:96731NEWT:383379NEWT:418106NEWT:10029NEWT:913645NEWT:44491NEWT:641809NEWT:27933NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:3112NEWT:79329NEWT:4442NEWT:31155NEWT:192875NEWT:1214915NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:108061NEWT:60711NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:160621NEWT:212790NEWT:931281NEWT:4432NEWT:5762NEWT:5763NEWT:658457NEWT:1310161NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NEWT:2246NEWT:2652724NEWT:1912919NEWT:5757NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:752555NEWT:498211NEWT:5759NEWT:1736231NEWT:5518NEWT:398007NEWT:1392NEWT:498217NEWT:498216NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:1367269NEWT:145458NEWT:633149NEWT:10239NEWT:68895NEWT:44685NEWT:161934NEWT:4897NEWT:1148NEWT:5744NEWT:5508NEWT:3329NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1235816NEWT:1140NCBITaxon:2157NEWT:1143NEWT:4896NEWT:1287689NEWT:1390NEWT:11557NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:294NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:3711NEWT:935293NEWT:375146NEWT:2065263NEWT:1772NEWT:118698NEWT:1616117NEWT:263NEWT:118696NEWT:52283NEWT:11988NEWT:284812NEWT:8175NEWT:400667NEWT:43330NEWT:980415NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:3702NEWT:243277NEWT:1246791NEWT:7067NEWT:118694NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:7070NCBITaxon:287NEWT:408172NEWT:408170NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:1436733NEWT:460519NEWT:1247411NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:1348799NEWT:9483NEWT:333760NEWT:56636NEWT:1513458NEWT:1233681NCBITaxon:40559NEWT:506599NEWT:2853422NEWT:81077NEWT:84588NEWT:1679718NEWT:480NEWT:65349NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:109760NEWT:943274NEWT:644223NEWT:235443NEWT:108458NEWT:5936NEWT:272623NEWT:272624NEWT:320637NEWT:2719099NEWT:983964NEWT:118499NEWT:11706NEWT:32644NEWT:527796NEWT:1130798NEWT:55529NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1116234NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:3983NEWT:352472NEWT:1071661NEWT:1202532NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:1218097NCBITaxon:50557NEWT:39251NEWT:39491NCBITaxon:5811NEWT:29491NEWT:101841NEWT:2014887NEWT:33952NEWT:153009NEWT:261756NEWT:571256NEWT:40483NEWT:63366NEWT:63367NEWT:688333NEWT:215402NCBITaxon:548681NEWT:318586NEWT:272634NEWT:9031NEWT:1872122NEWT:1308NEWT:7091NEWT:1034015NEWT:760568NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:97477NEWT:7111NEWT:347515NEWT:172269NEWT:9534NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:8694NEWT:4093NEWT:4096NEWT:8692NEWT:2903NEWT:185579NEWT:941442NEWT:536088NEWT:13076NEWT:1226408NEWT:7108NEWT:40479NEWT:43989NEWT:4076NEWT:142615NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:552NEWT:4084NEWT:63577NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:114155NEWT:6494NEWT:6491NEWT:507601NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:8654NEWT:8658NEWT:1268063NEWT:8655NEWT:5147NEWT:98351NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:207559NEWT:407821NCBITaxon:2NEWT:85057NEWT:985076NEWT:568708NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:267671NEWT:28532NEWT:198618NEWT:353152NEWT:8496NEWT:7165NEWT:27448NEWT:40674NEWT:1194669NEWT:51329NEWT:243230NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:1328388NEWT:104395NEWT:9557NEWT:6289NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:9554NEWT:118562NEWT:725NEWT:7137NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:2925NEWT:214053NEWT:80863NEWT:90675NEWT:105231NEWT:104385NEWT:74649NEWT:52638NEWT:57075NEWT:206672NEWT:4097NEWT:8697NEWT:8695NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:9545NEWT:7370NEWT:9793NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:255470NEWT:27457NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:6669NEWT:9935NEWT:89453NEWT:287889NEWT:1114301NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:419610NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NCBITaxon:10239NCBITaxon:714NEWT:295027NCBITaxon:11320NEWT:632957NEWT:11051NEWT:9925NEWT:89462NCBITaxon:9606NEWT:452680NEWT:8839NEWT:90690NEWT:1436183NEWT:4232NEWT:2758385NEWT:416348NEWT:11298NEWT:171101NEWT:714NEWT:5322NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:553470NEWT:242507NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:999810NCBITaxon:5693NEWT:28995NEWT:1392998NEWT:380394NEWT:114796NEWT:981087NEWT:180066NEWT:226900NEWT:1266738NEWT:231490NEWT:244704NEWT:7725NEWT:430615NEWT:563041NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:35024NEWT:135461NEWT:1678078NEWT:7962NEWT:749906NEWT:418985NEWT:1817808NEWT:749907NEWT:145262NEWT:145263NEWT:69014NEWT:142809NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:8643NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:8644NEWT:36329NEWT:62615NEWT:5141NEWT:46360NCBITaxon:3044782NEWT:1333550NEWT:160235NEWT:72407NEWT:8639NEWT:1928434NEWT:8637NEWT:349741NEWT:409859NEWT:157295NEWT:2096NEWT:509169NEWT:6210NEWT:3051137NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:409860NEWT:2785785NEWT:411901NEWT:8626NEWT:105884NCBITaxon:9615NEWT:8621NEWT:58334NEWT:452659NEWT:1193501NEWT:58331NEWT:6689NEWT:8624NEWT:5116NEWT:8623NEWT:5597NCBITaxon:3077NEWT:298315NEWT:8618NEWT:70448NEWT:9704NEWT:698936NEWT:8855NEWT:9940NEWT:3168NEWT:1247190NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:2126719NEWT:224911NEWT:118705NCBITaxon:11552NEWT:334249NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:6943NEWT:2NEWT:157546NEWT:870726NEWT:474186NEWT:38942NEWT:1493NEWT:307972NEWT:1496NEWT:2102NEWT:3674NEWT:660469NEWT:1365186NEWT:38949NEWT:45474NEWT:1299114NEWT:1590841NEWT:2282334NEWT:4513NEWT:1247NEWT:70667NEWT:869728NEWT:5843NEWT:6938NEWT:376741NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:557436NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:1085956NEWT:5833NEWT:3659NEWT:6928NEWT:1239NEWT:36745NEWT:499229NEWT:400727NEWT:272563NEWT:11009NEWT:79220NEWT:82688NEWT:5823NEWT:3885NEWT:3888NEWT:5821NEWT:44139NEWT:649408NCBITaxon:4895NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:12993NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:316407NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:1123294NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:4555NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:288705NEWT:29176NEWT:4787NEWT:3217NEWT:4547NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:154293NEWT:156471NEWT:4784NEWT:3694NEWT:360106NEWT:156476NEWT:4543NEWT:4785NEWT:360104NEWT:1857017NEWT:10117NEWT:10359NEWT:10116NEWT:1280NEWT:10358NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:718308NEWT:4530NEWT:54126NEWT:75058NEWT:13616NEWT:172NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:215358NEWT:64152NEWT:4924NEWT:749200NEWT:2748NEWT:8049NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:203124NEWT:192952NEWT:230741NEWT:8296NEWT:117008NCBITaxon:1313NEWT:167748NEWT:44544NEWT:529818NEWT:400540NEWT:4911NEWT:645463NEWT:152480NEWT:1886NEWT:129249NEWT:1077286NEWT:943129NEWT:1221594NEWT:990119NEWT:77216NEWT:1111708NEWT:227321NEWT:493760NEWT:281677NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:1162296NEWT:106592NEWT:46704NEWT:482428NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1423772NEWT:53232NEWT:1639NEWT:188229NEWT:246437NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:9103NEWT:715877NEWT:862238NEWT:29159NEWT:10306NEWT:9595NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3633NEWT:3635NEWT:5811NEWT:3879NEWT:180923NEWT:1328881NEWT:411483NEWT:76336NEWT:3870NEWT:884019NEWT:198215NEWT:411490NEWT:200298NEWT:169963NEWT:587NEWT:588NEWT:585NEWT:343NEWT:225117NEWT:746128NEWT:5801NEWT:43665NEWT:212035NCBITaxon:1653394NEWT:1266464NEWT:1205NEWT:367830NEWT:178616NEWT:375451NEWT:357NEWT:9172NEWT:2010198NEWT:1277351NEWT:457428NEWT:106178NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:569NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:28035NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:62141NEWT:1423NEWT:4932NEWT:3603NEWT:2756NEWT:2759NEWT:3848NEWT:3847NEWT:38293NEWT:1428NEWT:339NEWT:327159NEWT:178876NEWT:1660NEWT:2751NEWT:327160NEWT:573NEWT:571NEWT:12922NEWT:241368NEWT:518766NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:509924NEWT:115104NEWT:1121114NEWT:663NEWT:8570NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:220668NEWT:1821314NEWT:1249668NEWT:426430NEWT:9407NEWT:9646NEWT:1808362NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:9644NEWT:415540NEWT:675060NEWT:334542NEWT:313625NEWT:27592NEWT:426428NEWT:227289NEWT:63459NEWT:1276815NEWT:40324NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:242231NEWT:197221NEWT:7215NEWT:2172103NCBITaxon:59201NEWT:7460NEWT:504457NEWT:434271NEWT:747679NEWT:66712NEWT:42752NEWT:103372NEWT:746360NEWT:862751NEWT:89764NEWT:470150NEWT:9627NEWT:543734NEWT:1009714NEWT:73239NEWT:38018NEWT:226186NEWT:9870NEWT:8782NEWT:115357NEWT:435590NEWT:1902NEWT:1908NEWT:1169740NEWT:13164NEWT:216129NEWT:2664179NEWT:623NEWT:7038NEWT:6192NCBITaxon:1639NCBITaxon:38727NEWT:74790NEWT:2829NEWT:366581NEWT:216599NEWT:405534NEWT:216595NEWT:40559NEWT:51208NEWT:216597NEWT:9689NEWT:8355NEWT:633NEWT:1607839NEWT:9685NEWT:7029NEWT:6182NEWT:1283300NEWT:9694NEWT:186990NEWT:632NEWT:1204551NEWT:6183NEWT:99586NEWT:630NCBITaxon:11019NEWT:334747NEWT:61235NEWT:392021NEWT:343691NEWT:1457551NEWT:15368NEWT:1491466NEWT:436486NEWT:340188NEWT:51655NEWT:170187NEWT:260707NEWT:121845NEWT:8595NEWT:7263NEWT:186763NEWT:185431NEWT:29760NEWT:260704NEWT:1719NEWT:703612NEWT:260705NEWT:692672NEWT:2697049NEWT:1505907NEWT:1216981NEWT:1109443NEWT:1249885NEWT:7004NEWT:1972855NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1415167NEWT:1329349NEWT:607699NEWT:426688NEWT:208963NEWT:208964NEWT:27337NEWT:575584NEWT:185687NEWT:3039NEWT:669202NEWT:2706014NEWT:8727NEWT:8726NEWT:10090NEWT:8729NEWT:4120NEWT:8720NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:37334NEWT:5697NCBITaxon:10357NCBITaxon:10114NCBITaxon:10359NEWT:1685931NEWT:1203258NEWT:242619NEWT:49317NEWT:557723NEWT:557722NEWT:160791NEWT:1353008NEWT:34073NEWT:696811NEWT:402042NEWT:373995NEWT:5689NEWT:8715NEWT:544404NEWT:92519NEWT:2173NEWT:943925NEWT:37349NEWT:4113NEWT:837NEWT:640459NEWT:36015NEWT:1232801NEWT:81866NEWT:5691NEWT:563192NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:61674NEWT:2072580NEWT:1117957NEWT:6526NEWT:8709NEWT:4100NEWT:1159556NEWT:5671NEWT:1076NEWT:4102NEWT:4101NEWT:6763NEWT:8700NEWT:3498NEWT:803NEWT:29722NEWT:1692259NEWT:1486917NEWT:419947NEWT:468911NEWT:36470NEWT:817NEWT:7604NEWT:818NEWT:135588NEWT:1843183NEWT:5661NEWT:157592NEWT:51866NEWT:29719NEWT:58002NEWT:5665NEWT:6754NEWT:6997NEWT:452589NEWT:509068NEWT:5666NEWT:4577NEWT:813NEWT:5664NEWT:2157NEWT:146479NEWT:1042986NEWT:1911079NEWT:69373NEWT:1843192NEWT:206189NEWT:427577NCBITaxon:620NEWT:264203NEWT:1480154NEWT:1274414NEWT:9612NEWT:38626NEWT:1286640NEWT:38865NEWT:3077NEWT:9615NEWT:10299NEWT:6100NEWT:713604NEWT:112273NEWT:1147787NEWT:62977NEWT:1440772NEWT:10040NEWT:1355477NEWT:8759NEWT:9605NEWT:9606NEWT:90371NEWT:641501NEWT:4153NEWT:9601NEWT:278839NEWT:35128NEWT:39966NEWT:7668NEWT:8756NEWT:915099NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:1143193NEWT:1122233NEWT:890382NEWT:174934NEWT:179392NEWT:2823525NEWT:115778NEWT:9838NEWT:9839NEWT:546414NEWT:227086NEWT:3055NEWT:11167NEWT:9835NEWT:4146NEWT:5478NEWT:6326NEWT:7656NEWT:5476NEWT:1450520NEWT:1042311NEWT:1123352NEWT:1882945NEWT:299767NEWT:235909NEWT:28835NEWT:47311NEWT:1274432NEWT:2053667NEWT:1274426NEWT:104660NEWT:9825NEWT:1274423NEWT:393765NEWT:652611NEWT:39946NEWT:9823NEWT:95602NEWT:8732NEWT:314539NEWT:39947NEWT:718274NEWT:1274420NEWT:578458NEWT:6052042520931