Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/Supplementary_Table_2.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/Supplementary_Table_3.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/Supplementary_Table_1.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/ProteinGroups_File_WP_HEMK1.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/ProteinGroups_File_MEP_HEMK1.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/DIA-NN_report_MEP_HEMK1.pdfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/DIA-NN_report_WP_HEMK1.pdfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20251117_CB_HEK_HMTI1KO_E5_rep1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250510_AJ_HEK_F8_rep2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_WP_rep2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_WP_rep3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250510_AJ_HEK_F8_rep3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_mito_rep4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20251117_CB_HEK_HMTI1KO_F8_rep2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250510_AJ_HEK_E5_rep1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_WP_rep1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_mito_rep3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250510_AJ_HEK_E5_rep2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20251117_CB_HEK_HMTI1KO_F8_rep1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_mito_rep1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250822_MD_AJ_HEK_WT_mito_rep2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250510_AJ_HEK_E5_rep3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20250510_AJ_HEK_F8_rep1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080370/20251117_CB_HEK_HMTI1KO_E5_rep2.rawprimaryOK200franziska.traube@ibc.uni-stuttgart.deFranziska TraubeMass SpectrometryData-independent acquisitionBottom-up proteomicsmitochondrial translationHemk1translation termination factorggq loop methylationprotein glutamine methyltransferaseHemk1; protein glutamine methyltransferase; protein glutamine methylation; translation termination factor; mitochondrial translation; ggq loop methylationprotein glutamine methylationhttps://www.ebi.ac.uk/pride/archive/projects/PXD080370Whole-proteome samples lysis for proteomic analysis HEK293 WT and HEMK1 KO cells were lysed in NP-40 lysis buffer containing 1% (v/v) NP-40, 0.2% (w/v) SDS, and 20 mM HEPES (pH 7.5), supplemented with 500 U Benzonase (Merck Millipore), Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) and 2 mM DTT. Cell pellets containing approximately 1.5 × 10^6 cells were resuspended in 100 µL whole-proteome lysis buffer and incubated on ice for 30 min. Samples were then sonicated in a water-bath sonicator for three cycles of 30 s on and 30 s off, followed by centrifugation at 21,000 × g for 10 min at 4 °C. The cleared supernatants were transferred to fresh tubes, and protein concentrations were determined using a BCA assay. Mitochondrial enrichment for proteomic analysis Mitochondria were isolated from HEK293 WT cells and HEMK1 KO clones 3E5 and 1F8 using the Mitochondria Isolation Kit for Tissue and Cultured Cells (Abcam) according to the manufacturer's instructions. Briefly, cell pellets containing approximately 2 × 107 cells were resuspended in Mitochondria Isolation Buffer supplemented with protease inhibitors and incubated on ice. Permeabilization Reagent was then added, and samples were incubated on ice with intermittent vortexing. Cell debris and nuclei were removed by centrifugation at 600 × g for 10 min at 4 °C. The resulting supernatant was centrifuged at 7,000 × g for 10 min at 4 °C to pellet mitochondria. The mitochondrial pellet was washed with Mitochondria Isolation Buffer and resuspended in Storage Buffer II. Protein concentrations were determined using a BCA. Proteomic sample preparation Protein concentrations of whole-cell lysates and mitochondria-enriched fractions were determined using the Pierce™ BCA Protein Assay (Thermo Scientific) according to the manufacturer's instructions. Samples and corresponding lysis buffers were diluted 1:15 in water, and BSA standards ranging from 0 to 2000 µg/mL were used for calibration. Samples were incubated with BCA working reagent for 30 min at 37 °C, and absorbance was measured at 562 nm. For LC–MS/MS analysis, 20 µg of protein per sample were processed using the SP3 method as previously described. Briefly, proteins were bound to carboxylate-coated magnetic beads in the presence of ethanol and washed three times with 80% (v/v) ethanol. On-bead reduction and alkylation were performed in 100 mM ammonium bicarbonate containing DTT and iodoacetamide for 5 min at 95 °C. After cooling, proteins were digested overnight at 37 °C with sequencing-grade trypsin. Peptides were recovered from the supernatant, and additional washes with 0.1% formic acid were pooled with the peptide fraction. Residual beads were removed by magnetic separation, and peptide samples were transferred to LC–MS vials for LC–MS/MS analysis. LC–MS/MS acquisition LC–MS/MS acquisition was performed on an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific) coupled to an UltiMate 3000 Nano HPLC system (Thermo Fisher Scientific) via an EASY-Spray source and FAIMS interface (Thermo Fisher Scientific) in data-independent acquisition (DIA) mode. Per sample, 150 ng of peptides was first loaded on Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 A, 300 μm ID × 5 mm; Thermo Fisher Scientific) and was then separated at 40°C on a PicoTip emitter (non-coated, 15 cm, 75 μm ID, 8 μm tip; New Objective) that was in-house–packed with the Reprosil-Pur 120 C18-AQ material (1.9 μm, 150 Å, Dr. A. Maisch GmbH). For whole-proteome samples, a gradient over 60 min and 0.1% (vol/vol) FA in LC-MS grade water as buffer A and 0.1% (vol/vol) FA in MeCN as buffer B were used with a flow rate of 0.3 μL/min and 0 – 5 min 4% B, then from 5 – 6 min to 7% B, followed by 6 – 36 min to 24.8% B, 36 – 41 min to 35.2% B. From 41 – 41.1 min, B was increased to 80% until 46 min, when column was re-equilibrated at 4% B until 55 min. For mitochondria-enriched proteome samples, a gradient over 150 min and 0.1% (vol/vol) FA in LC-MS grade water as buffer A and 0.1% (vol/vol) FA in MeCN as buffer B were used with a flow rate of 0.3 μL/min and 0 – 5 min 4% B, then from 5 – 6 min to 7% B, followed by 6 – 105 min to 24.8% B, 105 – 126 min to 35.2% B. From 126 – 126.1 min, B was increased to 80% until 131 min, when column was re-equilibrated at 4% B until 131.1 min. The DIA duty cycle consisted of one MS1 scan followed by 30 MS2 scans with an isolation window of the 4 m/z range, overlapping with an adjacent window at the 2 m/z range. MS1 scan was conducted with Orbitrap at 60,000 resolution power and a scan range of 200 – 1,800 m/z with an adjusted RF lens at 30%. MS2 scans were conducted with Orbitrap at 30,000 resolution power, and RF lens was set to 30%. The precursor mass window was restricted to a 500 – 740 m/z range. HCD fragmentation was enabled as activation type with a fixed collision energy of 35%. FAIMS was performed with one CV at −45 V for both MS1 and MS2 scans during the duty cycle.PrideAnalysis of MS spectra, differential protein abundance and GO term enrichment analysis MS raw files were processed, separately for whole-proteome and mitochondrial enrichment samples, in DIA-NN (v. 1.8.1)to determine the protein identity and quantity in each sample. FASTA digest for library-free search/library generation was activated, and a FASTA spectral library generated for homo sapiens from UniProt (uniprot.org) was used. As protease, trypsin/P was chosen, the missed cleavages being allowed were set to 2, and the minimal peptide length was set to 6. MBR (match between runs) mode was enabled and heuristic protein inference was disabled. As quantification strategy, Robust LC (high precision) was used and threads were set to 7. Otherwise, default settings were used. From the DIA-NN output, the protein group file was uploaded to and subsequently analyzed using MaxQuant–Perseus (v2.0.11). For the mitochondrial enrichment samples, WT replicate #1 was discarded due to the high number of missing values compared to other WT samples, indicating reduced sample quality. Samples were grouped according to the genotype (samples that are biologically independent but of the same type). Proteins had to be present in at least >50% of the samples within at least one sample group or were otherwise completely discarded from the analysis to avoid bias by a large number of missing values over all experiments. For the remaining proteins, LFQ intensities were log2-transformed and missing values were replaced from normal distribution (separately for each column, default settings, Perseus). The WT and HEMK1 KO samples were compared against each other as indicated in the figures using the volcano plot function in Perseus (t test, both-sided, 250 randomizations, FDR 0.05. We applied significance thresholds of −log(p-value) > 1.3 (corresponding to p-value < 0.05) and |log2FC| > 0.58496 (corresponding to fold change > 1.5). For mitochondrial proteome analysis, mitochondrial proteins were assigned according to MitoCarta3.0. GO term enrichment analysis was performed using GOrilla.ProteomicsFranziska TraubePARTIALUniversity of Stuttgart, Institute of BiochemistryHomo Sapiens (human)franziska.traube@cup.lmu.deNot availableIBTB - University of StuttgartGermanyfalseHuman HEMK1 methylates all mitochondrial release factors and is required for mitochondrial function under stress conditionsHEMK1 is the mitochondrial homolog of HEMK2, the enzyme that methylates the conserved glutamine in the GGQ loop of eukaryotic translation termination factor eRF1. Previous work reported altered GGQ-loop glutamine methylation of mitochondrial release factors (MRF) after HEMK1 deletion, but direct biochemical evidence for HEMK1 catalytic activity and the functional consequences of MRF methylation for mitochondrial translation and physiology remained unclear. Here, we show that HEMK1 methylates all four human MRFs, with MTRF1L being the preferred substrate, consistent with MTRF1L’s reported role as the main stop-codon-dependent mitochondrial termination factor. We generated HEMK1 knockout HEK293 cells and analyzed their transcriptome and proteome, but detected only limited effects on the global level. In contrast, we observed that many mitochondrially encoded transcripts and proteins were consistently reduced. This decrease was generally stronger at the protein than at the transcript level, supporting a direct role of HEMK1 in mitochondrial translation. The accompanying reduction in mitochondrial transcripts may be a secondary consequence of impaired mitochondrial translation leading to mRNA destabilization via mRNA quality control. HEMK1 knockout clones showed increased sensitivity to mitochondrial stress, including elevated populations with low mitochondrial inner membrane potential and signs of apoptosis. These data indicate that loss of HEMK1 causes a latent mitochondrial defect that is tolerated under standard culture conditions but becomes apparent during mitochondrial or metabolic stress.2026-07-032026-06-30PXD080370NEWT:330879NEWT:377960NEWT:1131NEWT:2042546NEWT:259447NEWT:295546NCBITaxon:2719036NEWT:1334565NEWT:625351NEWT:258594NCBITaxon:1280NEWT:112503NEWT:1129NEWT:309807NEWT:59753NEWT:89184NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:1073882NEWT:6819NEWT:475174NEWT:111205NEWT:1117NEWT:498257NEWT:10036NEWT:1590NEWT:10034NEWT:33114NEWT:661410NEWT:638632NEWT:224326NEWT:376619NCBITaxon:79857NEWT:1096976NEWT:3765NEWT:3767NEWT:1589NEWT:135622NEWT:35786NEWT:1580NEWT:399784NEWT:96731NEWT:656064NEWT:1582NEWT:1584NEWT:36630NEWT:383379NEWT:418106NCBITaxon:12721NEWT:10029NEWT:913645NEWT:641809NEWT:1392488NEWT:38815NEWT:317447NEWT:4688NEWT:111225NEWT:7719NEWT:868565NEWT:135674NEWT:79329NEWT:30069NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:108061NEWT:60711NEWT:224308NEWT:3347NEWT:160621NEWT:212790NEWT:110368NEWT:1310161NEWT:77133NEWT:145481NEWT:1310165NEWT:29058NCBITaxon:79824NEWT:1912919NEWT:410658NEWT:5516NEWT:44688NEWT:44689NEWT:498211NEWT:347256NEWT:5518NEWT:398007NEWT:1527468NEWT:498217NEWT:498216NEWT:11320NEWT:246196NEWT:246197NEWT:145458NEWT:1310173NEWT:44685NEWT:161934NEWT:156587NEWT:1148NEWT:5508NEWT:3329NEWT:419481NEWT:5507NEWT:410661NEWT:1140NCBITaxon:2157NEWT:1143NEWT:1287689NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:150808NEWT:1182590NEWT:3712NEWT:3711NEWT:3714NEWT:270643NEWT:2065263NEWT:177437NEWT:10418NEWT:118698NEWT:1616117NEWT:118696NEWT:34865NEWT:52283NEWT:28174NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:44664NEWT:43346NEWT:3702NEWT:1245466NEWT:3704NEWT:244366NEWT:1246791NEWT:176597NEWT:118694NEWT:2850NEWT:118691NEWT:34871NEWT:33548NEWT:52299NEWT:8165NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:460519NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:1348799NEWT:33995NEWT:55784NEWT:1303443NEWT:9483NEWT:485NEWT:56636NEWT:2709072NEWT:2499525NEWT:2853422NEWT:487NEWT:1538553NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:1185650NEWT:216778NEWT:294607NEWT:1502NEWT:128017NEWT:376686NEWT:237610NEWT:95486NEWT:1883446NEWT:1233435NEWT:118223NEWT:11757NEWT:987059NEWT:109760NEWT:29031NEWT:317047NEWT:491NEWT:460523NEWT:45954NEWT:235443NEWT:1757312NEWT:85694NEWT:109786NEWT:108458NEWT:5936NEWT:320637NEWT:1078283NEWT:3750NEWT:983964NEWT:11706NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:3747NEWT:1116234NEWT:1255228NEWT:410289NEWT:373153NEWT:1640278NEWT:472NEWT:940825NEWT:529507NEWT:1071661NEWT:28128NEWT:470NEWT:5911NEWT:748693NCBITaxon:50557NEWT:39251NEWT:29491NEWT:101841NEWT:446NEWT:153481NEWT:2014887NEWT:33952NEWT:445NEWT:153009NEWT:261756NEWT:166313NEWT:63366NEWT:63367NEWT:215402NEWT:1547NEWT:906914NEWT:28141NEWT:9031NEWT:27292NEWT:1050722NEWT:3728NEWT:108931NEWT:1293497NEWT:1055524NEWT:150475NEWT:267872NEWT:381666NEWT:172269NEWT:9534NEWT:5180NEWT:256737NEWT:9541NEWT:8694NEWT:33936NEWT:8692NEWT:2903NEWT:185579NEWT:13076NEWT:207340NEWT:1006581NEWT:8673NEWT:33940NEWT:550NEWT:554NEWT:451516NEWT:552NEWT:1325291NEWT:42410NEWT:36185NEWT:1054211NEWT:1225786NEWT:771870NEWT:51338NEWT:575412NEWT:28112NEWT:6493NEWT:10832NEWT:6494NEWT:6491NEWT:507601NEWT:520NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:13095NEWT:162425NEWT:104105NEWT:216257NEWT:9986NEWT:8654NEWT:8658NEWT:1268063NEWT:8657NEWT:9983NEWT:8655NEWT:5147NEWT:595495NEWT:28108NEWT:28104NEWT:407821NEWT:568703NCBITaxon:2NEWT:568708NEWT:1613NEWT:986NEWT:52641NEWT:28532NEWT:353152NEWT:298176NEWT:353153NEWT:227377NEWT:1774284NEWT:40674NEWT:1194669NEWT:51329NEWT:443906NEWT:519NEWT:2510939NEWT:7394NEWT:6063NEWT:1328388NEWT:105487NEWT:1548728NEWT:667127NEWT:9557NEWT:377586NEWT:300641NEWT:39655NEWT:9554NEWT:38323NEWT:475932NEWT:256318NEWT:206411NCBITaxon:6191NEWT:229533NEWT:214053NEWT:28550NEWT:80863NEWT:90675NEWT:52639NEWT:52638NEWT:57075NEWT:1666905NEWT:8697NEWT:861109NEWT:8695NEWT:884204NEWT:510516NEWT:1045775NEWT:1123869NEWT:9544NEWT:9545NEWT:979NEWT:7370NEWT:83906NEWT:99488NEWT:1134506NEWT:255470NEWT:38783NEWT:6426NEWT:33090NEWT:9935NEWT:287889NEWT:305959NEWT:1028307NEWT:13489NEWT:29829NEWT:92867NEWT:92866NCBITaxon:3055NEWT:51750NEWT:202950NEWT:441772NEWT:1293091NEWT:48106NEWT:202956NEWT:295027NCBITaxon:11320NEWT:632957NEWT:9925NCBITaxon:9606NEWT:90690NEWT:1436183NEWT:300273NEWT:4232NEWT:416348NEWT:11298NEWT:4236NEWT:7742NEWT:196627NEWT:1076878NEWT:200308NEWT:200302NEWT:870435NEWT:58370NEWT:9913NEWT:9915NEWT:105841NEWT:2666255NEWT:999810NCBITaxon:5693NEWT:28995NEWT:2144189NEWT:1392998NEWT:380394NEWT:1150621NEWT:226900NEWT:1266738NEWT:231490NEWT:244704NEWT:7725NEWT:9909NEWT:9902NEWT:430615NEWT:563041NEWT:72664NEWT:36599NEWT:41949NEWT:249161NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1678078NEWT:749906NEWT:418985NEWT:749907NEWT:150847NEWT:29835NEWT:431947NEWT:69014NEWT:142809NEWT:302409NEWT:130821NEWT:27606NEWT:1519788NEWT:59202NEWT:9975NEWT:8643NEWT:1801631NEWT:1159899NEWT:502780NEWT:860688NEWT:13443NEWT:35231NEWT:1774208NEWT:8644NEWT:1125630NEWT:5141NEWT:46360NCBITaxon:3044782NEWT:202914NEWT:160235NEWT:502779NEWT:297246NEWT:103690NEWT:8639NEWT:1928434NEWT:8637NEWT:1114970NEWT:2096NEWT:285272NEWT:192222NEWT:9963NEWT:1617910NEWT:197911NEWT:197912NEWT:637379NEWT:12107NEWT:2785785NEWT:411901NEWT:1298722NEWT:8626NEWT:186801NEWT:105884NCBITaxon:9615NEWT:8621NEWT:58334NEWT:58331NEWT:6446NEWT:8624NEWT:5116NEWT:6447NEWT:8623NEWT:5597NCBITaxon:3077NEWT:194439NCBITaxon:12637NEWT:38727NEWT:218496NEWT:8618NEWT:70448NEWT:300251NEWT:9945NEWT:9940NEWT:13451NEWT:1247190NEWT:118702NEWT:118700NEWT:2126719NEWT:118705NEWT:1079921NEWT:6945NEWT:2104NEWT:2627626NEWT:6943NEWT:157546NEWT:870726NEWT:91374NEWT:91373NEWT:1414448NEWT:474186NEWT:54195NEWT:10136NEWT:38942NEWT:4762NEWT:6941NEWT:307972NEWT:2102NEWT:34NEWT:38949NEWT:1299114NEWT:1590841NEWT:60876NEWT:1276473NEWT:2282334NEWT:1247NEWT:70667NEWT:869728NEWT:6938NCBITaxon:39054NEWT:1736309NEWT:4751NEWT:36774NEWT:194963NEWT:77298NEWT:15819NEWT:109461NEWT:1085956NEWT:6928NEWT:1239NEWT:36745NEWT:400727NEWT:272560NEWT:272563NEWT:11009NEWT:913998NEWT:68335NEWT:3885NEWT:3888NEWT:649408NEWT:6915NEWT:101510NEWT:3880NEWT:272559NEWT:216816NEWT:153151NEWT:2681598NEWT:515849NEWT:10141NEWT:95767NEWT:466585NEWT:1000589NEWT:85963NEWT:29NEWT:85962NEWT:42197NEWT:143361NEWT:5656NEWT:1292NEWT:1299NEWT:424718NEWT:316407NEWT:1794699NCBITaxon:183674NEWT:167387NEWT:84645NEWT:1123294NEWT:6978NEWT:209285NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:1000561NEWT:253836NEWT:2133NEWT:6973NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:406072NEWT:1454958NEWT:351660NEWT:29176NEWT:201319NEWT:1073569NEWT:4787NEWT:751585NEWT:113549NEWT:180788NEWT:159749NEWT:1270NEWT:388919NEWT:130081NEWT:4784NEWT:360106NEWT:4785NEWT:360104NEWT:10117NEWT:401614NEWT:10116NEWT:1280NEWT:83333NEWT:83334NEWT:242718NEWT:83332NEWT:161NEWT:242715NEWT:2951803NEWT:100226NEWT:1263NEWT:176299NEWT:54126NEWT:75058NEWT:13616NEWT:13613NEWT:172NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1034304NEWT:105023NEWT:866628NEWT:535026NEWT:749200NEWT:1036967NEWT:266264NEWT:272942NEWT:272943NEWT:1882682NEWT:145953NEWT:257309NEWT:33657NEWT:203124NEWT:306254NEWT:230741NEWT:8296NEWT:117008NEWT:167748NEWT:1163394NEWT:645463NEWT:749696NEWT:320549NEWT:990119NEWT:77216NEWT:1111708NEWT:998086NEWT:493760NEWT:93163NEWT:257313NEWT:3827NEWT:400772NEWT:257310NEWT:128161NEWT:930945NEWT:332521NEWT:106350NEWT:29170NEWT:269796NEWT:54571NEWT:412754NEWT:9598NEWT:52389NEWT:2665964NEWT:1423772NEWT:51056NEWT:35815NEWT:53232NEWT:1235987NCBITaxon:2509511NEWT:1639NEWT:188229NEWT:3818NEWT:432359NEWT:9103NEWT:715877NEWT:862238NEWT:29159NEWT:1155748NEWT:9593NEWT:8260NEWT:9595NEWT:2665958NEWT:246200NEWT:6087NEWT:145943NEWT:595536NEWT:1134822NEWT:240906NEWT:89920NEWT:3809NEWT:29158NEWT:44744NEWT:3879NEWT:1328881NEWT:411483NEWT:76336NEWT:3870NEWT:884019NEWT:411490NEWT:169963NEWT:587NEWT:588NEWT:585NCBITaxon:43925NEWT:746128NEWT:212035NCBITaxon:1653394NEWT:1205NEWT:214693NEWT:45609NEWT:239935NEWT:1680NEWT:280148NEWT:9172NEWT:2770979NEWT:112NEWT:423477NEWT:1277351NEWT:1594786NEWT:40366NEWT:3854NEWT:411469NEWT:1679NEWT:333849NEWT:214684NEWT:1758543NEWT:569NEWT:411464NEWT:411460NEWT:1328426NEWT:562NEWT:411470NEWT:10515NEWT:2094720NCBITaxon:2697049NEWT:294750NEWT:1663NEWT:214675NEWT:62141NEWT:65651NEWT:3848NEWT:3847NEWT:198466NEWT:327159NEWT:1045010NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:1131757NEWT:571NEWT:142479NEWT:29595NEWT:241368NEWT:42528NEWT:1969471NEWT:9657NEWT:150596NEWT:30540NEWT:264483NEWT:439733NEWT:76777NEWT:30545NEWT:76773NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:1504802NEWT:1821314NEWT:103351NEWT:40354NEWT:1249668NEWT:426430NEWT:38033NEWT:37181NEWT:929439NEWT:9646NEWT:5286NEWT:7469NEWT:9644NEWT:426428NEWT:63459NEWT:2652157NEWT:2652158NEWT:40353NEWT:1276815NEWT:40324NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:693977NEWT:242231NEWT:197221NEWT:528354NEWT:51453NEWT:1279085NEWT:28231NEWT:7460NEWT:504457NEWT:392227NEWT:1004024NEWT:103372NEWT:862751NEWT:470150NEWT:9627NEWT:1009714NEWT:73239NEWT:38018NEWT:226186NEWT:381518NEWT:30532NEWT:8782NEWT:30538NEWT:115357NEWT:5270NEWT:63436NEWT:63433NEWT:13164NEWT:1601067NEWT:129554NEWT:40302NEWT:1249615NEWT:343232NEWT:343237NEWT:2664179NEWT:623NEWT:217219NEWT:7038NEWT:6192NCBITaxon:1639NEWT:3915NEWT:1581419NCBITaxon:3570232NEWT:405534NEWT:9689NEWT:8355NEWT:633NEWT:9685NEWT:1218948NEWT:7029NEWT:9688NEWT:6182NEWT:9694NEWT:604162NEWT:1046723NEWT:186990NEWT:8364NEWT:6185NEWT:632NEWT:6183NEWT:1423800NEWT:630NCBITaxon:11019NEWT:334747NEWT:3907NEWT:3906NEWT:61235NEWT:343691NEWT:1457551NEWT:15368NEWT:13180NEWT:1491466NEWT:340188NEWT:170187NEWT:1755437NEWT:1042399NEWT:9681NEWT:1719NEWT:692672NEWT:2697049NEWT:568813NEWT:1109443NEWT:267566NEWT:58042NEWT:7004NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1329349NEWT:1089444NEWT:607699NEWT:6161NEWT:206542NEWT:27337NEWT:575584NEWT:186536NEWT:257771NEWT:185687NEWT:3039NEWT:2706014NEWT:8727NEWT:8726NEWT:8725NEWT:10090NEWT:8729NEWT:8720NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:8722NEWT:5697NEWT:6544NEWT:218192NCBITaxon:10114NEWT:2190NEWT:1685931NEWT:242619NEWT:557723NEWT:557722NEWT:160791NEWT:1353008NEWT:34073NEWT:402042NEWT:373995NEWT:5689NEWT:8715NEWT:544404NEWT:287535NEWT:2173NEWT:2175NEWT:1232801NEWT:5691NEWT:61674NEWT:2072580NEWT:669465NEWT:1117957NEWT:6526NEWT:8709NEWT:1064539NEWT:8707NEWT:1306274NEWT:5671NEWT:13146NEWT:6523NEWT:8700NEWT:1692259NEWT:1486917NEWT:2636997NEWT:1843183NEWT:5661NEWT:157592NEWT:3482NEWT:52714NEWT:51866NEWT:58002NEWT:13136NEWT:5660NEWT:5665NEWT:1179777NEWT:6997NEWT:5666NEWT:1746091NEWT:5664NEWT:2157NEWT:70125NEWT:146479NEWT:1843192NEWT:206189NEWT:2160NEWT:295139NEWT:181214NEWT:6108NEWT:1720309NEWT:9612NEWT:1286640NEWT:38865NEWT:3077NEWT:9615NEWT:6100NEWT:713604NEWT:3076NEWT:112273NEWT:33178NEWT:1147787NEWT:39717NEWT:62977NEWT:1440772NEWT:45157NEWT:10040NEWT:8759NEWT:9605NEWT:9606NEWT:27717NEWT:641501NEWT:9601NEWT:8752NEWT:34038NEWT:9604NEWT:8756NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:439375NEWT:1857323NEWT:890382NEWT:174934NEWT:179392NEWT:2823525NEWT:115778NEWT:1538186NEWT:82996NEWT:227086NEWT:3055NEWT:195883NEWT:1450520NEWT:1042311NEWT:1123352NEWT:741258NEWT:82525NEWT:29920NEWT:235909NEWT:47311NCBITaxon:813NEWT:127785NEWT:708248NEWT:47314NEWT:104660NEWT:652611NEWT:94993NEWT:8732NEWT:763037NEWT:1250232NEWT:1052588NEWT:1377NEWT:3555NEWT:71647NEWT:260201NEWT:35554NCBITaxon:12939NEWT:935692NEWT:32046NEWT:2222NEWT:544496NEWT:1736657NEWT:59529NEWT:32049NEWT:1392696NEWT:762376NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:3544NEWT:5722NEWT:7901NEWT:32051NEWT:10245NEWT:55153NEWT:10243NEWT:351581NEWT:43186NEWT:428146NEWT:889247NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:32025NEWT:2649997NEWT:1147161NEWT:43150NEWT:69781NEWT:263737NEWT:1333499NEWT:5702NEWT:95648NEWT:1349NEWT:67593NEWT:100787NEWT:1401257NEWT:44491NEWT:27933NEWT:556484NEWT:7955NEWT:7959NEWT:645159NEWT:2261NEWT:94323NEWT:31156NEWT:3112NEWT:8801NEWT:4442NEWT:192875NEWT:1214915NEWT:1214916NEWT:284218NEWT:1316931NEWT:511145NEWT:213401NEWT:931281NEWT:4432NEWT:5762NEWT:5763NEWT:118099NEWT:32019NEWT:658457NEWT:420247NEWT:35525NEWT:295358NEWT:2246NEWT:2652724NEWT:5757NEWT:68415NCBITaxon:4563NEWT:5755NEWT:44447NEWT:752555NEWT:39097NEWT:347495NEWT:5759NEWT:1736231NEWT:1392NEWT:35974NEWT:2242NEWT:1396NEWT:286NEWT:391619NEWT:287NEWT:1367269NEWT:633149NEWT:1149786NEWT:10239NEWT:68895NEWT:3567NEWT:68888NEWT:4897NEWT:5744NEWT:72901NEWT:213863NEWT:55571NEWT:35500NEWT:1235816NEWT:2041044NEWT:3562NEWT:4896NEWT:1262161NEWT:1314781NEWT:1390NEWT:11557NEWT:294NEWT:1773NEWT:82380NEWT:935293NEWT:375146NEWT:471876NEWT:1772NEWT:263NEWT:11988NEWT:400667NEWT:408169NEWT:36809NEWT:47946NEWT:459758NEWT:1764NEWT:39087NEWT:413996NEWT:243277NEWT:7067NEWT:586722NEWT:243273NEWT:1986198NEWT:274NEWT:7070NCBITaxon:287NEWT:408172NEWT:408170NEWT:1436733NEWT:42229NEWT:1247411NEWT:9238NEWT:13708NEWT:333760NEWT:34607NEWT:1513458NEWT:1233681NEWT:2212966NCBITaxon:40559NEWT:506599NEWT:81077NEWT:84588NEWT:1279007NEWT:85433NEWT:572545NEWT:65349NEWT:300852NEWT:564933NEWT:508771NEWT:1121405NEWT:253NCBITaxon:2759NEWT:93061NEWT:93062NEWT:1284813NEWT:34613NEWT:548470NEWT:7054NCBITaxon:4932NEWT:1748NEWT:108673NEWT:2764168NEWT:943274NEWT:1488406NEWT:644223NEWT:1263082NEWT:272623NEWT:1288389NEWT:272624NEWT:886559NEWT:55521NEWT:118499NEWT:1130798NEWT:55529NEWT:256136NEWT:476272NEWT:195051NEWT:3988NEWT:561007NEWT:649908NEWT:1001135NEWT:147828NEWT:3981NEWT:3983NEWT:203696NEWT:223283NEWT:352472NEWT:235NEWT:505235NEWT:55518NEWT:97441NEWT:1154756NEWT:1202531NEWT:1202532NEWT:360094NEWT:1408223NEWT:1408224NEWT:41364NEWT:1311NEWT:1313NEWT:1218097NEWT:1080348NEWT:1314NEWT:39491NEWT:212142NCBITaxon:5811NEWT:3972NEWT:1499105NEWT:192812NEWT:408188NEWT:571256NEWT:40483NEWT:688333NEWT:1119529NEWT:1303NCBITaxon:548681NEWT:318586NCBITaxon:5820NEWT:272634NEWT:198107NEWT:1979401NEWT:155892NEWT:691NEWT:637910NEWT:28384NEWT:167879NEWT:1872122NEWT:1308NEWT:7091NEWT:1034015NEWT:760568NEWT:141262NEWT:1813NEWT:4087NEWT:9778NEWT:306NEWT:303NEWT:97477NEWT:7111NEWT:113287NEWT:347515NEWT:6269NEWT:4090NEWT:4092NEWT:4093NEWT:786NEWT:4096NEWT:208895NEWT:941442NEWT:536088NEWT:311553NEWT:1226408NEWT:43984NEWT:7108NEWT:40479NEWT:43989NEWT:4076NEWT:142615NEWT:317NEWT:885319NEWT:293939NEWT:497515NEWT:885318NEWT:39488NEWT:1268285NEWT:4081NEWT:273068NEWT:1316582NEWT:227166NEWT:98334NEWT:231663NEWT:4084NEWT:63577NEWT:7574NEWT:1715256NEWT:30640NEWT:348824NEWT:929793NEWT:1507179NEWT:29204NEWT:114155NEWT:4072NEWT:1337056NEWT:4073NEWT:634955NEWT:6239NEWT:102169NEWT:208893NEWT:4054NEWT:301301NEWT:98351NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:63558NEWT:61376NEWT:207559NEWT:107401NEWT:85057NEWT:985076NEWT:1215323NEWT:7159NEWT:267671NEWT:72491NEWT:7160NEWT:8496NEWT:7165NEWT:1257118NEWT:1387333NEWT:8010NEWT:1315282NEWT:7162NEWT:1859NEWT:27448NEWT:2708NEWT:235612NEWT:243232NEWT:243230NEWT:936053NEWT:1080772NEWT:8479NEWT:8485NEWT:119856NEWT:72000NEWT:119857NEWT:49240NEWT:2893330NCBITaxon:3707NEWT:391295NEWT:1170828NEWT:104395NEWT:93934NEWT:9315NEWT:6289NEWT:6287NEWT:38569NEWT:727NEWT:9796NEWT:118562NEWT:725NEWT:7137NEWT:469008NEWT:9321NEWT:596153NEWT:8472NEWT:1836NCBITaxon:10298NEWT:1652998NEWT:105231NEWT:104385NEWT:74649NEWT:1037924NEWT:206672NEWT:9788NEWT:4097NEWT:9305NEWT:436113NEWT:71139NEWT:9785NEWT:6279NEWT:1195774NEWT:9793NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:27457NEWT:4006NEWT:1872635NCBITaxon:5476NEWT:113208NEWT:6669NEWT:1215360NEWT:89453NEWT:1114301NEWT:51515NEWT:13249NEWT:588596NEWT:51511NEWT:8845NEWT:7998NEWT:419610NEWT:5334NEWT:382352NEWT:413071NCBITaxon:10239NEWT:332097NCBITaxon:714NEWT:1076620NEWT:11051NEWT:89462NEWT:452680NEWT:8839NEWT:4472NEWT:2758385NEWT:171101NEWT:714NEWT:5322NEWT:34199NEWT:568996NEWT:421932NEWT:312017NEWT:576131NEWT:553470NCBITaxon:10243NEWT:284593NEWT:100392NEWT:655819NEWT:5315NEWT:11082NEWT:265872NEWT:59463NEWT:337330NEWT:2285NEWT:2287NEWT:6645NEWT:251229NEWT:981087NEWT:180066NEWT:46147NCBITaxon:6312NEWT:11072NEWT:174633NEWT:52830NEWT:1416333NEWT:35024NEWT:135461NEWT:7962NEWT:99287NEWT:1817808NEWT:145262NEWT:145263NEWT:694569NEWT:9739NEWT:111069NEWT:1379791NEWT:51953NEWT:57267NEWT:3197NEWT:13686NEWT:2750454NEWT:264561NEWT:1402135NEWT:36329NEWT:62615NEWT:1555636NEWT:1333550NEWT:646099NEWT:72407NEWT:352914NEWT:349741NEWT:409859NEWT:157295NEWT:1263871NEWT:509169NEWT:6210NEWT:3051137NEWT:9721NEWT:137221NEWT:565050NEWT:409860NEWT:92683NEWT:1298968NEWT:5353NEWT:1034809NEWT:452659NEWT:1193501NEWT:51930NEWT:6689NEWT:194675NEWT:298315NEWT:392547NEWT:46105NEWT:104782NEWT:10181NEWT:9704NEWT:1049056NEWT:698936NEWT:13216NEWT:1009874NEWT:4498NEWT:8855NEWT:11034NEWT:3168NEWT:10185NEWT:78168NEWT:521001NEWT:192011NEWT:340912NEWT:1813887NEWT:224911NEWT:1090946NCBITaxon:11552NEWT:267608NEWT:334249NEWT:184922NEWT:6703NEWT:2NEWT:35670NEWT:511693NEWT:4528NEWT:35677NEWT:136841NEWT:1493NEWT:10376NEWT:1496NEWT:3674NEWT:5851NEWT:660469NEWT:935568NEWT:1365186NEWT:1813019NEWT:45474NEWT:48500NEWT:4513NEWT:5843NEWT:110205NEWT:1117645NEWT:376741NCBITaxon:10407NEWT:1000364NEWT:10360NEWT:1403536NEWT:1427524NEWT:47664NEWT:260799NEWT:317548NEWT:106198NEWT:2323NEWT:3654NEWT:237561NEWT:5833NEWT:3659NEWT:59620NEWT:499229NEWT:31298NEWT:1204162NEWT:1532734NEWT:82688NEWT:5823NEWT:5821NEWT:44139NCBITaxon:4895NEWT:570417NCBITaxon:4896NEWT:675512NEWT:315393NEWT:3649NEWT:28903NEWT:325176NEWT:28909NEWT:3641NEWT:79698NCBITaxon:5796NEWT:12993NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:170721NEWT:34305NEWT:77020NEWT:1908323NEWT:83558NEWT:44586NEWT:626528NEWT:139927NEWT:4558NEWT:5888NEWT:431241NEWT:4550NEWT:4555NEWT:144036NEWT:144030NEWT:288705NEWT:392815NEWT:3217NEWT:4547NEWT:3218NEWT:556182NEWT:374990NEWT:36941NEWT:1432059NEWT:154293NEWT:156471NEWT:3694NEWT:5875NEWT:156476NEWT:4543NEWT:210007NEWT:1857017NEWT:10359NEWT:1358430NEWT:10358NEWT:1381693NEWT:1735272NEWT:109298NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:1917265NEWT:55210NEWT:718308NEWT:4530NEWT:5861NEWT:1895NEWT:2558360NEWT:215358NEWT:64152NEWT:4924NEWT:2748NEWT:8049NEWT:33892NEWT:99931NEWT:9378NEWT:990346NEWT:100816NEWT:192952NEWT:1640346NCBITaxon:1313NEWT:29385NEWT:44544NEWT:529818NEWT:400540NEWT:382NEWT:4911NEWT:152480NEWT:129249NEWT:1888NEWT:1077286NEWT:943129NEWT:131567NEWT:1221594NEWT:10760NEWT:1077281NEWT:1524260NEWT:227321NEWT:192944NEWT:492670NEWT:281677NEWT:106590NEWT:260710NEWT:4918NEWT:1162296NEWT:106592NEWT:596645NEWT:46704NEWT:42345NEWT:482428NEWT:1187947NEWT:4903NEWT:10312NCBITaxon:1773NEWT:190304NEWT:1432499NEWT:8030NEWT:246432NEWT:246437NEWT:36909NEWT:4909NEWT:443256NEWT:1279365NEWT:759272NEWT:1182263NEWT:943146NEWT:646532NEWT:2711NEWT:537457NEWT:479249NEWT:435623NEWT:33889NEWT:10306NEWT:12967NEWT:8022NEWT:42353NEWT:593117NEWT:3633NEWT:319761NEWT:3635NEWT:5811NEWT:86662NEWT:180923NEWT:1451NEWT:198215NEWT:200298NEWT:12916NEWT:1400870NEWT:343NEWT:84005NEWT:225117NEWT:34708NEWT:8090NEWT:537012NEWT:5801NEWT:43665NEWT:5802NEWT:4952NEWT:1266464NEWT:367830NEWT:1093790NEWT:178616NEWT:95145NEWT:358NEWT:375451NEWT:54788NEWT:357NEWT:74381NEWT:2010198NEWT:457428NEWT:106178NEWT:84023NEWT:589904NEWT:559292NEWT:2762NEWT:1174673NEWT:28035NEWT:1313166NEWT:690307NCBITaxon:138NEWT:28038NEWT:1422NEWT:84030NEWT:1423NEWT:4932NEWT:1205903NEWT:3603NEWT:2756NEWT:2759NEWT:38293NEWT:1428NEWT:339NEWT:2751NEWT:330NEWT:139077NEWT:391904NEWT:12922NEWT:316277NEWT:163159NEWT:1306413NEWT:2813599NEWT:518766NEWT:2664373NEWT:190802NEWT:1213463NEWT:7234NEWT:9417NEWT:1216979NEWT:9895NEWT:7237NEWT:5059NEWT:55601NEWT:31631NEWT:509924NEWT:28450NEWT:115104NEWT:5061NEWT:7240NEWT:8570NEWT:127569NEWT:5062NEWT:1408434NEWT:1241935NEWT:220668NEWT:3428464NEWT:9407NEWT:7227NEWT:193079NEWT:9402NEWT:415540NEWT:336722NEWT:675060NEWT:416870NEWT:334542NEWT:313625NEWT:51240NEWT:27592NEWT:5052NEWT:227289NEWT:1985738NEWT:7215NEWT:196139NEWT:5037NEWT:651182NEWT:2172103NCBITaxon:59201NEWT:7220NEWT:1876254NEWT:434271NEWT:1915NEWT:747679NEWT:66712NEWT:42752NEWT:746360NEWT:89764NEWT:543734NEWT:49390NEWT:9870NEWT:256648NEWT:435590NEWT:1902NEWT:208526NEWT:1908NEWT:1169740NEWT:217459NEWT:216129NEWT:1245012NEWT:171250NEWT:528135NEWT:1637254NCBITaxon:38727NEWT:176946NEWT:74790NEWT:2829NEWT:126279NEWT:1228997NEWT:1428006NEWT:62335NEWT:366581NEWT:757417NEWT:216599NEWT:216595NEWT:40559NEWT:51208NEWT:216597NEWT:42736NEWT:1607839NEWT:1283300NEWT:1218707NEWT:1204551NEWT:99586NEWT:228577NEWT:2060328NEWT:48038NEWT:392021NEWT:436486NEWT:51655NEWT:1933298NEWT:260707NEWT:121845NEWT:8595NEWT:7263NEWT:186763NEWT:447135NEWT:185431NEWT:216591NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:1202347NEWT:1295743NEWT:546805NEWT:1505907NEWT:1216981NEWT:1249885NEWT:1972855NEWT:853NEWT:7244NEWT:1415167NEWT:36036NCBITaxon:662NEWT:9430NEWT:851NEWT:71251NEWT:208963NEWT:208964NEWT:181088NEWT:48498NEWT:669202NEWT:1193806NEWT:6306NEWT:4120NEWT:37334NCBITaxon:10357NEWT:2040765NCBITaxon:10358NCBITaxon:10359NEWT:1203258NEWT:49317NEWT:1899318NEWT:696811NEWT:9808NEWT:839NEWT:9803NEWT:1097670NEWT:92519NEWT:943925NEWT:4111NEWT:37349NEWT:4113NEWT:837NEWT:640459NEWT:72568NEWT:8954NEWT:36015NEWT:51641NEWT:35168NEWT:81866NEWT:334147NEWT:1262469NEWT:277944NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:712938NEWT:3258NEWT:112018NEWT:1906951NEWT:52945NEWT:4100NEWT:1159556NEWT:1076NEWT:4102NEWT:1028688NEWT:4101NEWT:6763NEWT:803NEWT:29722NEWT:419947NEWT:2847306NEWT:468911NEWT:13373NEWT:266834NEWT:817NEWT:7604NEWT:6756NEWT:818NEWT:135588NEWT:1061NEWT:29719NEWT:6754NEWT:452589NEWT:509068NEWT:2794344NEWT:4577NEWT:813NEWT:1365218NEWT:337677NEWT:1042986NEWT:1911079NEWT:69373NEWT:427577NEWT:264203NEWT:1480154NEWT:9858NEWT:9859NEWT:512623NEWT:1274414NEWT:2907833NEWT:38626NEWT:10298NEWT:10299NEWT:1087439NEWT:9852NEWT:9860NEWT:660025NEWT:61408NEWT:1355477NEWT:90371NEWT:55194NEWT:2907829NEWT:4153NEWT:11137NEWT:278839NEWT:35128NEWT:39966NEWT:7668NEWT:2929635NEWT:58228NEWT:915099NEWT:1143193NEWT:687914NEWT:1122233NEWT:277988NEWT:859313NEWT:59540NEWT:9838NEWT:9839NEWT:546414NEWT:11167NEWT:9835NEWT:7650NEWT:4146NEWT:5478NEWT:6326NEWT:7656NEWT:5476NEWT:5477NEWT:1882945NEWT:746707NEWT:299767NEWT:28835NEWT:1206781NEWT:5480NEWT:1274432NEWT:222863NEWT:2053667NEWT:859321NEWT:1274426NEWT:9825NEWT:1274423NEWT:393765NEWT:104421NEWT:39946NEWT:9823NEWT:95602NEWT:314539NEWT:39947NEWT:718274NEWT:1274420NEWT:859314NEWT:578458NEWT:605200000-0002-5691-1941