{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR320/DRR320431/DRR320431_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR320/DRR320431/DRR320431_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"strain":["BW25113"],"omics_type":["Genomics"],"center_name":["Graduate School of Engineering, The University of Tokyo"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJDB12336"],"scientific_name":["Escherichia coli BW25113"],"long_description":["In the early stage of translation elongation, peptidyl-tRNA (pep-tRNA) with short nascent chain frequently dissociates from the ribosome (pep-tRNA drop-off). Inactivation of PTH (peptidyl-tRNA hydrolase) causes rapid accumulation of pep-tRNAs and depletion of aminoacyl(aa)-tRNAs, leading to defective protein synthesis. We established a highly sensitive method for direct profiling of pep-tRNAs accumulated in the cell and identified more than 700 peptide sequences of the pep-tRNAs. In this experiment, to examine the involvement of RRF in pep-tRNA drop-off in the early elongation stage, we performed ribosome profiling of WT and temperature-sensitive RRF strain incubated at 43°C for 30 min."],"tag":["pathogen:priority","pathogen:bacterium","pathogen"],"classification":["bacteria"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Escherichia coli BW25113","description":"Ribosome profiling analyses on WT and RRFTs strain","dates":{"last_updated":"2025-09-24","first_public":"2023-05-21"},"accession":"PRJDB12336","cross_references":{"taxon":["679895"]}}