ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636247/DRR636247_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636244/DRR636244_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636245/DRR636245_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636246/DRR636246_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636247/DRR636247_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636244/DRR636244_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636245/DRR636245_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR636/DRR636246/DRR636246_1.fastq.gzprimaryOK200GenomicsDepartment of Urology, Graduate School of Biomedical and Health Sciences, Hiroshima universityhttps://www.ebi.ac.uk/ena/browser/view/PRJDB20188Homo sapiensTo investigate the functional role of KDM6A in renal cell carcinoma (RCC), we established KDM6A-knockout (KO) cell lines by employing CRISPR/Cas9-mediated disruption of the KDM6A gene in 786-O and Caki-1 wild-type (WT) cells. Comparative RNA sequencing analyses were performed to identify molecular pathways associated with KDM6A deficiency. Total RNA was extracted from both KDM6A-KO and WT control cells and processed into libraries using the SureSelect Strand-Specific RNA Library Preparation Kit (Agilent Technologies). Transcriptome analysis was conducted with the HiSeq 2500 next-generation sequencer (Illumina), and the resulting sequence reads were aligned to the human genome (hg38). The RNA sequencing dataset, including samples from KDM6A-KO 786-O, KDM6A-KO Caki-1, WT 786-O, and WT Caki-1 cells, will be publicly available.ENAfalseHomo sapiensGene Expression Alterations in KDM6A-Knockout Renal Cell Carcinoma Cells2025-09-242025-01-30PRJDB201889606