ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639651/DRR639651_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639656/DRR639656_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639651/DRR639651_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639654/DRR639654_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639656/DRR639656_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639652/DRR639652_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639652/DRR639652_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR639/DRR639654/DRR639654_2.fastq.gzftp://primaryOK200GenomicsTakaragaike-1Ishizaki lab Graduate School of Science, Kobe Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJDB20224Marchantia polymorphaThis study explores the role of the R2R3-MYB transcription factor, SHOT GLASS (MpSTG), in the liverwort Marchantia polymorpha. MpSTG is identified as a crucial regulator of gemma cup development and is predominantly expressed in gemma cups, apical notches, and sexual reproductive organs. Loss-of-function mutants in MpSTG form abnormal structures lacking gemmae and fail to develop sexual reproductive organs. To identify factors downstream of MpSTG that regulate gemma cup development, transcriptomic changes in 1-week gemmalings of MpSTG-GR plants were analyzed using RNA sequencing. Differential gene expression analysis revealed that MpSTG overexpression induced by 2- and 8-hour DEX treatments upregulated 82 and 297 genes, respectively, while downregulating 36 and 164 genes. These findings suggest that MpSTG is a key transcription factor regulating both vegetative and sexual reproduction in M. polymorpha.ENAfalseMarchantia polymorpha strain:Takaragaike-1Role of the R2R3-MYB Transcription Factor SHOT GLASS in Promoting Gemma Cup Development and Gametangiophore Formation in the Liverwort Marchantia polymorpha2025-09-242025-06-29PRJDB202243197