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University of Tokyohttps://www.ebi.ac.uk/ena/browser/view/PRJDB6607TakifuguIn sequencing eukaryotic genomes, linkage maps are indispensable for building scaffolds with which to assemble and/or to validate chromosomes. However, current approaches to construct linkage maps are limited by marker density and cost-benefit ratio, especially for wild organisms. We have now devised a new strategy based on artificially generated hybrid organisms to acquire ultra high-density genomic markers at lower cost and build highly accurate linkage maps. Using this method, linkage maps and draft sequences for two species of pufferfish were obtained simultaneously. We anticipate that the method will accelerate genomic analysis of sexually reproducing organisms.ENAfalseTakifuguSimultaneous and highly efficient construction of two independent sets of high-density linkage maps and draft sequences using an artificially generated hybrid organism2023-05-172019-01-03PRJDB660731032