{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR993/ERR993504/ERR993504.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR993/ERR993505/ERR993505.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["COLORADO STATE UNIVERSITY"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB10584"],"long_description":["Alternative splicing (AS) and alternative polyadenylation (APA) of pre-mRNAs greatly contribute to transcriptome diversity, coding capacity of a genome and gene regulatory mechanisms in eukaryotes. Short-read sequencing of transcriptomes using second-generation sequencing has been extensively used to analyze gene expression and the extent of AS in eukaryotes. However, a major limitation with short-read data is that it is difficult to accurately predict the full-length splice isoforms produced from a gene. Here we sequenced the sorghum transcriptome using Pacific Biosciences single molecule real time long-read isoform sequencing (Iso-Seq) to identify full-length splice isoforms and APA sites. Analysis of these data revealed transcriptome-wide full-length isoforms at an unprecedented scale with over 8000 novel splice isoforms, first for any plant system. In addition, these data uncovered APA of about 5000 expressed genes, and >1400 novel genes that were not previously annotated. These results complement available sorghum genome in refining its transcriptome and study gene regulation in this important bioenergy crop."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"IsoSeq analysis of sorghum","description":"A survey of the sorghum transcriptome using single-molecule long reads","dates":{"last_updated":"2016-05-20","first_public":"2015-10-27"},"accession":"PRJEB10584","cross_references":{}}