ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR993/ERR993504/ERR993504.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR993/ERR993505/ERR993505.fastq.gzprimary200OKGenomicsCOLORADO STATE UNIVERSITYhttps://www.ebi.ac.uk/ena/browser/view/PRJEB10584Alternative splicing (AS) and alternative polyadenylation (APA) of pre-mRNAs greatly contribute to transcriptome diversity, coding capacity of a genome and gene regulatory mechanisms in eukaryotes. Short-read sequencing of transcriptomes using second-generation sequencing has been extensively used to analyze gene expression and the extent of AS in eukaryotes. However, a major limitation with short-read data is that it is difficult to accurately predict the full-length splice isoforms produced from a gene. Here we sequenced the sorghum transcriptome using Pacific Biosciences single molecule real time long-read isoform sequencing (Iso-Seq) to identify full-length splice isoforms and APA sites. Analysis of these data revealed transcriptome-wide full-length isoforms at an unprecedented scale with over 8000 novel splice isoforms, first for any plant system. In addition, these data uncovered APA of about 5000 expressed genes, and >1400 novel genes that were not previously annotated. These results complement available sorghum genome in refining its transcriptome and study gene regulation in this important bioenergy crop.ENAfalseIsoSeq analysis of sorghumA survey of the sorghum transcriptome using single-molecule long reads2016-05-202015-10-27PRJEB10584