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and Paris Cite Sorbonne University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB17508"],"long_description":["Background: Circulating tumor DNA (ctDNA) PCR-based methods have been approved at diagnosis or recurrence as companion tests for anti-EGFR therapies in lung cancer. However, the value of ctDNA to monitor treatment efficacy requires further evaluation. Using a newly validated Next-Generation Sequencing (NGS) approach we tested the clinical utility of ctDNA monitoring.Methods and Findings: We conducted a prospective observational study of ctDNA in monitoring patients with newly diagnosed advanced non-small cell lung cancers (NSCLC). The primary objective was the prognostic value of baseline ctDNA detection on overall survival. In this study 124 patients were included and plasma samples were informative at baseline (n=105), at first evaluation (n=85) and at tumor progression (n=66). CtDNA was assessed by ultra-deep targeted NGS using a dedicated variant caller algorithm. Common mutations were validated with digital PCR. Findings: We showed that optimized targeted NGS is a powerful method for ctDNA detection in clinics. We demonstrated that ctDNA is a prognostic and an early monitoring marker of treatment efficacy in NSCLC patients."],"repository":["ENA"],"description_synonyms":["Cancer of the lung, Malignant tumor of lung, Pulmonary Neoplasms, Lung cancer, Lung, DNS, Procedures, (Deoxyribonucleotide)n, Nucleobase, DNAn+1, malignant lung tumor, Neoplasms, lung cancer, Cancer of Lung, malignant tumor of lung, malignant neoplasm of the lung, Double-Stranded, Procedure, somatic, autosomal dominant, Deoxyribonucleic acids, (Deoxyribonucleotide)n+m, method, Techniques, Client., Deoxyribonucleic Acid, cancer of lung, Method, method used in an experiment, Base2, Base1, Studies, malignant lung tumour, Nonsmall cell lung cancer, Neoplasm, Malignant lung tumor, malignant tumour of lung, ds-DNA, Pulmonary Cancer, desoxyribose nucleic acid, Technique, Pulmonary cancer, Pulmonary, Lung Cancer, Lung malignant tumors, malignant tumor of the lung, thymus nucleic acid, malignant tumour of the lung, malignant lung neoplasm, Pulmonary Neoplasm, adenocarcinoma of lung, alveolar cell carcinoma, susceptibility to, bases, Basen, LNCR, Double Stranded, Deoxyribonucleic acid, Lung Cancers, Methodological, Cancers, Methodological Study, plan specification, Study, response to tyrosine kinase inhibitor in, nonsmall cell lung cancer, resistance to, Malignant neoplasm of lung, Methodological Studies, Lung Neoplasm, Patient, malignant neoplasm of lung, Clients, Base, ds DNA, Desoxyribonukleinsaeure, protection against, Double-Stranded DNA, Pulmonary Cancers, nucleobases, (Deoxyribonucleotide)m, DNA, deoxyribonucleic acids, Cancer of the Lung, lung neoplasm, DNAn, somatic mutation, base, Cancer"],"name_synonyms":["Cancer of the lung, Malignant tumor of lung, Pulmonary Neoplasms, Lung cancer, Lung, DNS, (Deoxyribonucleotide)n, DNAn+1, malignant lung tumor, Neoplasms, lung cancer, Cancer of Lung, malignant tumor of lung, malignant neoplasm of the lung, Double-Stranded, somatic, autosomal dominant, Deoxyribonucleic acids, (Deoxyribonucleotide)n+m, Deoxyribonucleic Acid, cancer of lung, malignant lung tumour, Nonsmall cell lung cancer, Neoplasm, Malignant lung tumor, malignant tumour of lung, ds-DNA, Pulmonary Cancer, desoxyribose nucleic acid, Pulmonary cancer, Pulmonary, Lung Cancer, Lung malignant tumors, malignant tumor of the lung, thymus nucleic acid, malignant tumour of the lung, malignant lung neoplasm, Pulmonary Neoplasm, adenocarcinoma of lung, alveolar cell carcinoma, susceptibility to, LNCR, Double Stranded, Deoxyribonucleic acid, Lung Cancers, Cancers, somatic., response to tyrosine kinase inhibitor in, nonsmall cell lung cancer, resistance to, Malignant neoplasm of lung, Lung Neoplasm, malignant neoplasm of lung, ds DNA, Desoxyribonukleinsaeure, protection against, Double-Stranded DNA, Pulmonary Cancers, (Deoxyribonucleotide)m, DNA, deoxyribonucleic acids, Cancer of the Lung, lung neoplasm, DNAn, somatic mutation, Cancer"],"additional_accession":[]},"is_claimable":false,"name":"NGS of circulating DNA in lung cancer","description":"Next-Generation-Sequencing with the Base-Position Error Rate (BPER) method on circulating DNA in lung cancer patients","dates":{"last_updated":"2016-10-31","first_public":"2016-12-30"},"accession":"PRJEB17508","cross_references":{}}