ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/003/ERR1855433/ERR1855433_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/002/ERR1855432/ERR1855432_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/004/ERR1855434/ERR1855434_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/005/ERR1855435/ERR1855435_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/007/ERR1855437/ERR1855437_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/006/ERR1855436/ERR1855436_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/002/ERR1855442/ERR1855442_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/003/ERR1855443/ERR1855443_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/006/ERR1855436/ERR1855436_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/007/ERR1855437/ERR1855437_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/005/ERR1855435/ERR1855435_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/001/ERR1855441/ERR1855441_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/000/ERR1855440/ERR1855440_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/009/ERR1855439/ERR1855439_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/008/ERR1855438/ERR1855438_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/000/ERR1855440/ERR1855440_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/003/ERR1855443/ERR1855443_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/004/ERR1855444/ERR1855444_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/002/ERR1855432/ERR1855432_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/003/ERR1855433/ERR1855433_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/004/ERR1855434/ERR1855434_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/004/ERR1855444/ERR1855444_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/009/ERR1855439/ERR1855439_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/008/ERR1855438/ERR1855438_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/002/ERR1855442/ERR1855442_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR185/001/ERR1855441/ERR1855441_1.fastq.gzprimaryOK200GenomicsWTCMPWellcome Trust Centre for Molecular Parasitology, University of Glasgow , UKhttps://www.ebi.ac.uk/ena/browser/view/PRJEB19634Kinome-focused RIT-seq: RNAi cell lines were generated as previously described [Jones NG, Thomas EB, Brown E, Dickens NJ, Hammarton TC, Mottram JC. Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen. PLoS pathogens. 2014 10(1):e1003886. doi: 10.1371/journal.ppat.1003886. PubMed PMID: 24453978 PubMed Central PMCID: PMC3894213]. RNAi lines were pooled, initially into 9 pools each containing 19-25 cell lines and frozen. These pools were then defrosted and further pooled and frozen. This stabilate was utilised in a variety of experiments to determine the role of protein kinases in a range of cell-cycle and DNA-repair conditions (see approrpriate papers for details) To recover the RNAi target sequences from the populations, a single universal primer (5’- TAATGCCAACTTTGTACAAA-3’) was used. This primer was barcoded with 14 different 6-nucleotide tags that permitted combining equal amounts of PCR products in a single sequencing sample. Reads were assigned to each experimental condition later in silico. 10 ng of genomic DNA obtained per sample was PCR- amplified in a 50 µl reaction using Q5® High-Fidelity DNA polymerase (NEB, Ipswich, USA). Groups of 14 barcoded PCR products were pooled in a single sequencing sample, and 400 ng processed according to Illumina Miseq library protocols.xref:EuropePMC:PMC5542689ENAfalseTrypanosome protein kinase studies by RITseq.Functional dissection of the 'kinome' (annotated protein kinase set) in Trypanosoma brucei by RITseq2017-02-232017-07-01PRJEB19634