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Cells were irradiated with one dose of 15 Gy ionizing X rays in a Faxitron FX-650 machine control cell culture dishes were not irradiated. After a day of radiation, adherent cells were detached by trypsinization and replated on 10-cm dishes in 8 ml RPMI-1640/+DMEM medium with 10% fetal bovine serum to reach 70%-90% confluence after three days, when adherent cells were harvested by scraping. Harvested cells were pelleted and kept frozen at -80 ºC. For each cell-line, three experiments were performed to obtain three pairs of control and irradiated cell pellets. One µg of total RNA isolated from each of the cell pellets using Norgen-Biotek Total RNA Isolation kit with an on-column DNAse I treatment step was used to prepare sequencing library for directional RNA sequencing to obtain paired 76 b reads on an Illumina NextSeq 500 instrument. Twenty-four libraries were sequenced in each flow lane, and each library was sequenced in four lanes of one flow cell. On-system software was used to demultiplex sequencing data.ENACancer of the lung, MAU2L, Malignant tumor of lung, Pulmonary Neoplasms, Lung cancer, Lung, human being, SCC4, RAY, malignant lung tumor, Neoplasms, Modern, lung cancer, Cancer of Lung, Gene, malignant tumor of lung, malignant neoplasm of the lung, somatic, autosomal dominant, Human, AU040256, H-ray, Homo sapiens, cancer of lung, ray, KIAA0892, head and neck carcinoma cell., malignant lung tumour, Nonsmall cell lung cancer, Neoplasm, Malignant lung tumor, malignant tumour of lung, scc4, Pulmonary Cancer, Man, Pulmonary cancer, Pulmonary, Radiations, Lung Cancer, Lung malignant tumors, malignant tumor of the lung, malignant tumour of the lung, malignant lung neoplasm, RAB1C, Gene Expressions, Man (Taxonomy), Pulmonary Neoplasm, adenocarcinoma of lung, alveolar cell carcinoma, susceptibility to, LNCR, Lung Cancers, Cancers, Expressions, human, kiaa0892, response to tyrosine kinase inhibitor in, nonsmall cell lung cancer, resistance to, Malignant neoplasm of lung, Lung Neoplasm, mau-2, malignant neoplasm of lung, Raja trachyderma, Modern Man, FaDu, protection against, Pulmonary Cancers, Expression, Cancer of the Lung, lung neoplasm, 9530019H02Rik, somatic mutation, CancerRadiations, MAU2L, primary cancer, SCC4, Malignant Neoplasm, Malignancy, Neoplasms, Benign Neoplasm, Benign Neoplasms, Cancers, Tumor, Malignant, malignant tumor, Malignant Neoplasms, kiaa0892, Neoplasias, MT, Benign, malignant neoplasm, mau-2, KIAA0892, FaDu, Neoplasm, Cell., scc4, Malignancies, Neoplasia, Cancer, TumorsfalseEffect of ionizing radiation on FaDu, H522, HSC2, and SCC4 cancer cellsEffect on gene expression after 4 days of a single, 15 Gy dose of ionizing X ray radiation on H522 human lung cancer, and FaDu, HSC2 and SCC4 human head and neck cancer cell-lines2017-03-312018-01-23PRJEB20193