ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR203/004/ERR2030974/ERR2030974.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR203/006/ERR2030976/ERR2030976.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR203/005/ERR2030975/ERR2030975.fastq.gzprimaryOK200GenomicsSTANFORD UNIVERSITYEuropean Bioinformatics Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJEB21724ArrayExpressxref:ArrayExpress:E-MTAB-5866We undertook a unbiased genome-wide haploid genetic screen to identify new components in interferon lambda signaling. In addition, we performed a genome-wide screen to identify genes that repress spontaneous activation of interferon stimulated genes in the absence of interferon. Both of these screens were performed using a HAP1 cell line containing GFP reporter under the transcriptional regulation of the Interferon-Stimulated Response Element from IFIT2. We also overexpressed IL28RA (IFNLR1) in this cell line, in order to sensitize the cells to type III interferonENAType III IFNs, Haploid Cells, Interferons, human being, wide/broad, Man (Taxonomy), Genomes, III IFNs, interferon lambda, Type III Interferons, Modern, Type III, type III interferon, IFN-Lambda, whole genome, III IFN, broad, interferon type III, Haploid Cell, man, Cell, Lambda, human, III Interferons, Type III Interferon, IFNs, Human, Type III IFN, Interferon Type, wide, Interferon-Lambda, III, III Interferon, signalling process, Homo sapiens, Haploids, Modern Man, Cells, Interferon Type III, Type, Cell., Haploid, Haploidies, Interferon, Man, single organism signaling, Lambda InterferonsType III IFNs, Haploid Cells, Interferons, human being, wide/broad, Man (Taxonomy), Genomes, III IFNs, interferon lambda, Type III Interferons, Modern, Type III, type III interferon, IFN-Lambda, whole genome, III IFN, broad, interferon type III, Haploid Cell, man, Cell, Lambda, human, III Interferons, Type III Interferon, IFNs, Human, Type III IFN, Interferon Type, wide, Interferon-Lambda, III, III Interferon, signalling process, Homo sapiens, Haploids, Modern Man, Cells, Interferon Type III, Type, Cell., Haploid, Haploidies, Interferon, Man, single organism signaling, Lambda InterferonsfalseHaploid genome-wide screens for essential components and negative regulators of interferon lambda signaling in human cellsHaploid genome-wide screens for essential components and negative regulators of interferon lambda signaling in human cells2017-07-112017-07-25PRJEB21724