ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045180/ERR045180_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045183/ERR045183_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045179/ERR045179_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR063/ERR063461/ERR063461_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045182/ERR045182_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045181/ERR045181_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045182/ERR045182_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045179/ERR045179_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045180/ERR045180_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045183/ERR045183_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR063/ERR063461/ERR063461_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR045/ERR045181/ERR045181_1.fastq.gzprimary200OKGenomicsTGACThe Genome Analysis Centrehttps://www.ebi.ac.uk/ena/browser/view/PRJEB2672Next generation sequencing (NGS) technologies are providing new ways to accelerate fine-mapping and gene isolation in many species. To date, the majority of these efforts have focused on diploid organisms with readily available whole genome sequence information. In this study, as a proof of concept, we tested the use of NGS for SNP discovery in tetraploid wheat lines differing for the previously cloned grain protein content (GPC) gene GPC-B1. Bulked segregant analysis (BSA) was used to define a subset of putative SNPs within the candidate gene region, which were then used to fine-map GPC-B1.ENAfalseNACombining SNP discovery from next-generation sequencing data with Bulked Segregant Analysis to fine-map genes in polyploid wheat2016-05-202012-01-11PRJEB2672