ENAGenomicsSouth African Sugarcane Research Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJEB29575Two novel primers were developed to sequence all the regions in Acetolactate synthase from grasses in which herbicide inhibiting mutations have been described. These primers were employed to characterize new ALS mutants in sugarcane. The primers were designed to be universal to true grasses and new ALS genes were amplified and sequenced for a range of species across the BOP and PACMAD clades.ENAfalseSequencing of Acetolactate Synthase genes in the grass family using novel universal primersDevelopment of novel universal primers in the true grasses to amplify and sequence the functional core of Acetolactate Synthase.2018-11-082021-01-13PRJEB29575