ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/003/ERR3672193/ERR3672193_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/002/ERR3672192/ERR3672192_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/005/ERR3672195/ERR3672195_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/001/ERR3672191/ERR3672191_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/002/ERR3672192/ERR3672192_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/000/ERR3672190/ERR3672190_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/008/ERR3672198/ERR3672198_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/004/ERR3672194/ERR3672194_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/001/ERR3672191/ERR3672191_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/009/ERR3672199/ERR3672199_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/006/ERR3672196/ERR3672196_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/009/ERR3672189/ERR3672189_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/009/ERR3672199/ERR3672199_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/003/ERR3672193/ERR3672193_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/007/ERR3672197/ERR3672197_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/008/ERR3672188/ERR3672188_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/006/ERR3672196/ERR3672196_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/009/ERR3672189/ERR3672189_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/008/ERR3672188/ERR3672188_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/007/ERR3672197/ERR3672197_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/004/ERR3672194/ERR3672194_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/000/ERR3672190/ERR3672190_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/008/ERR3672198/ERR3672198_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR367/005/ERR3672195/ERR3672195_2.fastq.gzprimaryOK200GenomicsEuropean Bioinformatics InstituteCentre for Genomic Regulation (CRG)https://www.ebi.ac.uk/ena/browser/view/PRJEB35489ArrayExpressUsing Mycoplasma pneumoniae as a model organism, we conditionally depleted the two essential ATP-dependent proteases (Lon and FtsH) of this bacterium, by engineering three strains carrying a Lon and/or FtsH inducible expression locus. An integrative comparative study combining label-free shotgun proteomics and RNA-seq allowed us to decipher the global cellular response to Lon and FtsH depletion and to define protease substrates in this genome-reduced organism.ENAMEG4, FTSH, DmelCG3576, lon, Dlag1, RNA-seq, LonHS, CG8798, PIM1, 1200017E13Rik, Eaton Agent., Lag1, DLag1, l(1)G0365, DLAG1, Ftsh, Prss15, LONP, DmelCG8798, YME1L, PRSS15, Lon, LON, lag1, l(1)G0489, CG3576, PAMP, Whole Transcriptome Shotgun Sequencing, hLON, anon-WO0118547.350, CG15898, l(1)G0061, FtsH1MEG4, FTSH, DmelCG3576, lon, Dlag1, RNA-seq, LonHS, CG8798, PIM1, 1200017E13Rik, Eaton Agent., Lag1, DLag1, l(1)G0365, DLAG1, Ftsh, Prss15, LONP, DmelCG8798, YME1L, PRSS15, Lon, LON, lag1, l(1)G0489, CG3576, PAMP, Whole Transcriptome Shotgun Sequencing, hLON, anon-WO0118547.350, CG15898, l(1)G0061, FtsH1falseRNA-seq of Lon and FtsH conditional mutants of Mycoplasma pneumoniae under depleting and inducing conditionsRNA-seq of Lon and FtsH conditional mutants of Mycoplasma pneumoniae under depleting and inducing conditions2019-11-202020-11-10PRJEB35489