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2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/005/ERR3833805/ERR3833805_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/000/ERR3833830/ERR3833830_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/005/ERR3833815/ERR3833815_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/007/ERR3833817/ERR3833817_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/005/ERR3833805/ERR3833805_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/002/ERR3833822/ERR3833822_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/009/ERR3833839/ERR3833839_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/004/ERR3833834/ERR3833834_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/007/ERR3833807/ERR3833807_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/009/ERR3833819/ERR3833819_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/001/ERR3833811/ERR3833811_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/006/ERR3833836/ERR3833836_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/004/ERR3833824/ERR3833824_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/001/ERR3833841/ERR3833841_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/008/ERR3833818/ERR3833818_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/005/ERR3833835/ERR3833835_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/003/ERR3833813/ERR3833813_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/002/ERR3833832/ERR3833832_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/000/ERR3833820/ERR3833820_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR383/007/ERR3833837/ERR3833837_2.fastq.gzprimaryOK200GenomicsUniversity of SouthamptonEuropean Bioinformatics Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJEB36346ArrayExpressHere we investigate the relevance of the microenvironment in follicular lymphoma by studying the effect of the lectin DC-SIGN, expressed by macrophages on B-cell receptor activation. We compare the effect of DC-SIGN and anti-IgM stimulation on FL by treating 3 FL samples with 20 μg/ml of goat F(ab’)2 anti-IgM, 20 μg/ml of DC-SIGN-Fc or left untreated for 4 hours at 37°C. Total RNA was extracted using an RNeasy mini kit (Qiagen) and polyA libraries were 75bp PE sequenced on a HiSeq4000 (Ilumina). After gene expression profiling analysis we found that, although DC-SIGN-Fc appears to elicit a weaker transcriptional response than anti-IgM, the responses are closely related and encompass a wide range of canonical BCR response pathways.ENAC-type lectin domain family 4 member L, Large Follicular Center-Cell, CIRE, Nodular Histiocytic Lymphomas, CD209a antigen, HIV-1-gp120 binding C-type lectin, Small Follicular Center-Cell Lymphoma, Brill-Symmers, Small Cleaved-Cell, Nodular Large Follicular Center Cell Lymphoma, Large Lymphoid Lymphoma, Follicular Large-Cell Lymphomas, Small Cleaved Cell Lymphoma, Mixed-Cell Lymphoma, Lymphomas, prevention and control, CLEC4L, Follicular, Mixed Small and Large Cell, Lymphocytic, Histiocytic Lymphoma, Follicular Lymphomas, reference sample, Mixed-Cell Lymphomas, Nodular Large Follicular Center-Cell Lymphoma, prevention., Small Follicular Center Cell, DC-SIGN, Follicular Mixed-Cell Lymphoma, Mixed Cell Lymphoma, Follicular Large-Cell Lymphoma, Small Follicular Center-Cell, preventive measures, Large-Cell, Large Cell Lymphoma, Mixed Small and Large Lymphoid, Large Follicular Center Cell, SIGNR5, Histiocytic Lymphomas, Poorly-Differentiated, Nodular, Clients, SIGN-R1, SIGNR1 protein, Dcsign, Mixed Lymphocytic-Histiocytic, Giant Follicular, Follicular Lymphoma, CD209, Cd209b protein, Controlled, Giant Follicular Lymphoma, Disease, Controlling, Small Lymphoid, preventive therapy, Follicular Large Cell, follicular, Large-Cell Lymphomas, mouse, Follicular Mixed-Cell, Small and Large Cleaved-Cell, CD209 antigen, Client, Small and Large Cleaved Cell, Mixed Lymphocytic Histiocytic, CDSIGN, HIV-1gp120 binding C-type lectin, Giant Follicular Lymphomas, Dendritic cell-specific ICAM-3-grabbing non-integrin, DC-SIGN protein, Nodular Lymphomas, Mixed Cell, Nodular Histiocytic Lymphoma, Brill Symmers Disease, Large Cell, Cd209a protein, Nodular Lymphoma, Giant, Large-Cell Lymphoma, DC-SIGN-related protein 4, DC-SIGN1, Histiocytic, prophylaxis, CD209b antigen, patient, Small Cleaved Cell, human, DC-specific ICAM-3 grabbing nonintegrin protein, OtB7, Grade 1, Grade 2, Follicular Mixed-Cell Lymphomas, Grade 3, Brill-Symmers Disease, lymphoma, Lymphocytic Lymphoma, Nodular Histiocytic, Patient, DCSIGN protein, Small Follicular Center Cell Lymphoma, control, Lymphoma, Follicular Large-Cell, DC-SIGNR4, Follicular Large Cell Lymphoma, SIGN-R1 protein, Poorly Differentiated, DC-SIGNR2, DC-SIGNR1, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, Large Lymphoid, Small Cleaved-Cell Lymphoma, CD209 protein, DC-SIGN-related protein 1, Mixed-Cell, Follicular Mixed Cell Lymphoma, DC-SIGN-related protein 2C-type lectin domain family 4 member L, Large Follicular Center-Cell, CIRE, Nodular Histiocytic Lymphomas, CD209a antigen, HIV-1-gp120 binding C-type lectin, Small Follicular Center-Cell Lymphoma, Brill-Symmers, Small Cleaved-Cell, Nodular Large Follicular Center Cell Lymphoma, Large Lymphoid Lymphoma, Follicular Large-Cell Lymphomas, Small Cleaved Cell Lymphoma, Mixed-Cell Lymphoma, Lymphomas, prevention and control, CLEC4L, Follicular, Mixed Small and Large Cell, Lymphocytic, Histiocytic Lymphoma, Follicular Lymphomas, reference sample, Mixed-Cell Lymphomas, Nodular Large Follicular Center-Cell Lymphoma, prevention., Small Follicular Center Cell, DC-SIGN, Follicular Mixed-Cell Lymphoma, Mixed Cell Lymphoma, Follicular Large-Cell Lymphoma, Small Follicular Center-Cell, preventive measures, Large-Cell, Large Cell Lymphoma, Mixed Small and Large Lymphoid, Large Follicular Center Cell, SIGNR5, Histiocytic Lymphomas, Poorly-Differentiated, Nodular, Clients, SIGN-R1, SIGNR1 protein, Dcsign, Mixed Lymphocytic-Histiocytic, Giant Follicular, Follicular Lymphoma, CD209, Cd209b protein, Controlled, Giant Follicular Lymphoma, Disease, Controlling, Small Lymphoid, preventive therapy, Follicular Large Cell, follicular, Large-Cell Lymphomas, mouse, Follicular Mixed-Cell, Small and Large Cleaved-Cell, CD209 antigen, Client, Small and Large Cleaved Cell, Mixed Lymphocytic Histiocytic, CDSIGN, HIV-1gp120 binding C-type lectin, Giant Follicular Lymphomas, Dendritic cell-specific ICAM-3-grabbing non-integrin, DC-SIGN protein, Nodular Lymphomas, Mixed Cell, Nodular Histiocytic Lymphoma, Brill Symmers Disease, Large Cell, Cd209a protein, Nodular Lymphoma, Giant, Large-Cell Lymphoma, DC-SIGN-related protein 4, DC-SIGN1, Histiocytic, prophylaxis, CD209b antigen, patient, Small Cleaved Cell, human, DC-specific ICAM-3 grabbing nonintegrin protein, OtB7, Grade 1, Grade 2, Follicular Mixed-Cell Lymphomas, Grade 3, Brill-Symmers Disease, lymphoma, Lymphocytic Lymphoma, Nodular Histiocytic, Patient, DCSIGN protein, Small Follicular Center Cell Lymphoma, control, Lymphoma, Follicular Large-Cell, DC-SIGNR4, Follicular Large Cell Lymphoma, SIGN-R1 protein, Poorly Differentiated, DC-SIGNR2, DC-SIGNR1, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, Large Lymphoid, Small Cleaved-Cell Lymphoma, CD209 protein, DC-SIGN-related protein 1, Mixed-Cell, Follicular Mixed Cell Lymphoma, DC-SIGN-related protein 2falseRNAseq of follicular lymphoma patient samples treated with anti-IgM, DC-SIGN or an isotype controlRNAseq of follicular lymphoma patient samples treated with anti-IgM, DC-SIGN or an isotype control2020-01-212020-01-31PRJEB36346