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Bioinformatics Institute","SHANGHAI JIAO TONG UNIVERSITY"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB36594"],"broker_name":["ArrayExpress"],"long_description":["Circulating tumor cells (CTCs) represent the molecular characteristics of tumor sites and travel in the blood for seeding distant metastases. \"EpCAM+/pan-cytokeratin (CK)+/CD45-/DAPI+\" has been widely accepted as a CTC definition, especially in breast cancer, prostate cancer and colorectal cancer. However, reports on CTC detection in non-small cell lung cancer are limited due to a lack of efficient CTC marker. We describe hexokinase 2 (HK2) that assays elevated glycolysis of cancer cells, called Warburg effect, as a new marker for CTC detection in lung adenocarcinoma (LUAD), especially the CK negative CTCs. Single-cell sequencing was used to confirm the malignancy of putative CTCs by detecting genome-wide copy number alternations characteristic of malignant cells. We employed this marker in a variety of liquid biopsies from LUAD patients, including peripheral blood, pleural effusion and cerebrospinal fluid."],"repository":["ENA"],"description_synonyms":["Hexokinase A, bronchogenic lung adenocarcinoma, Lung Adenocarcinomas, Adenocarcinoma, Hexokinase II, Hexokinase D, Lung, Malignant Neoplasm, Malignancy, Neoplasms, adenocarcinoma of lung, Benign Neoplasm, Benign Neoplasms, LUAD., non-small cell lung adenocarcinoma, Cancers, ATP:D-hexose 6-phosphotransferase, Tumor, Malignant, Adenocarcinomas, Cell, Malignant Neoplasms, Neoplasias, nonsmall cell adenocarcinoma, Lung Adenocarcinoma, adenocarcinoma of the lung, Benign, lung adenocarcinoma, hk1-A, Hexokinase, Neoplasm, Malignancies, other neoplasm, Neoplasia, Cancer, Tumors"],"name_synonyms":["Hexokinase A, bronchogenic lung adenocarcinoma, Lung Adenocarcinomas, Adenocarcinoma, Hexokinase II, Hexokinase D, Lung, Malignant Neoplasm, Malignancy, Neoplasms, adenocarcinoma of lung, Benign Neoplasm, Benign Neoplasms, LUAD., non-small cell lung adenocarcinoma, Cancers, ATP:D-hexose 6-phosphotransferase, Tumor, Malignant, Adenocarcinomas, Cell, Malignant Neoplasms, Neoplasias, nonsmall cell adenocarcinoma, Lung Adenocarcinoma, adenocarcinoma of the lung, Benign, lung adenocarcinoma, hk1-A, Hexokinase, Neoplasm, Malignancies, other neoplasm, Neoplasia, Cancer, Tumors"],"additional_accession":[]},"is_claimable":false,"name":"Hexokinase 2 detects cytokeratin negative circulating tumor cells in lung adenocarcinoma","description":"Hexokinase 2 detects cytokeratin negative circulating tumor cells in lung adenocarcinoma","dates":{"last_updated":"2020-02-05","first_public":"2021-03-01"},"accession":"PRJEB36594","cross_references":{}}