ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/004/ERR4769034/ERR4769034.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/003/ERR4769033/ERR4769033.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/005/ERR4769035/ERR4769035.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/000/ERR4769030/ERR4769030.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/007/ERR4769037/ERR4769037.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/002/ERR4769032/ERR4769032.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/001/ERR4769031/ERR4769031.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR476/006/ERR4769036/ERR4769036.fastq.gzprimaryOK200GenomicsEuropean Bioinformatics InstituteEndothelial Cell Biology Unit, Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuvenhttps://www.ebi.ac.uk/ena/browser/view/PRJEB41000ArrayExpressFemoral artery segments from the ligated ('L') right side (downstream of the ligation site) as well as the corresponding segments from the unligated ('UL') left side were dissected out 3 days after femoral artery ligation in adult mice with or without a long-term (from post-natal day 1 onwards) endothelial-specific deletion of transcription factor Prdm16. Fragments from 4 donor mice were pooled to obtain sufficient cell numbers for sequencing. The experiment existed of 4 samples to be sequenced at single-cell resolution (Prdm16 wild-type L or 'Right_WT' Prdm16 wild-type UL or 'Left_WT' Prdm16 endothelial-specific knockout L or 'Right_KO' Prdm16 endothelial-specific knockout UL or 'Left-KO'). The experiment was intended to identify in situ Prdm16 genotype-dependent genes and corresponding functional pathways in arterial endothelial cells that could be responsible for the observed endothelial dysfunction caused by endothelial-specific Prdm16 deletion in mice upon induction of hind limb ischemia.ENAfalsescRNAseq of femoral artery segments in the context of hind limb ischemia in mice with or without an endothelial cell-specific deletion of transcription factor Prdm16scRNAseq of femoral artery segments in the context of hind limb ischemia in mice with or without an endothelial cell-specific deletion of transcription factor Prdm162022-05-022022-05-02PRJEB41000