{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR705/007/ERR7059747/ERR7059747.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR705/000/ERR7059750/ERR7059750.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR705/008/ERR7059748/ERR7059748.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR705/006/ERR7059746/ERR7059746.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR705/009/ERR7059749/ERR7059749.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["UNIVERSITY OF ZURICH, UNIVERSITY HOSPITAL ZURICH"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB46898"],"long_description":["A high-throughput genome-wirde siRNA screening for lipoprotein uptake was performed on Huh-7 human hepatocarcinoma cells. In order to exclude possible false-positives coming from siRNA molecules targeting genes which are actually not expressed in Huh-7 cells, we determined basal gene expression in this cells line by RNASeq on an Illumina platform. Five samples were sequenced with increasing read depth in order to ensure that all low-expressed genes were detected. Expression levels from the sample with the highest read depth were used to filter the siRNA screening data."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"RNASeq to determine basal gene expression levels in Huh-7 human hepatocarcinoma cells","description":"RNASeq was performed on Huh-7 human hepatocarcinoma cells to determine basal gene expression levels. The data were then used to filter the results of a genome-wide siRNA screening for lipoprotein uptake.","dates":{"last_updated":"2021-11-01","first_public":"2021-11-01"},"accession":"PRJEB46898","cross_references":{}}