{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Yangzhou University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB52726"],"long_description":["CRISPR-Cas is an adaptive immune system that protects prokaryotes from the invasion of foreign genetic elements. The components and immunity mechanisms of CRISPR-Cas has been extensively studied, but the regulation of this system in Staphylococci remains unclear. Here, we show that the cell-cell communication, known as quorum sensing (QS) represses the expression and interference of Type III-A CRISPR-Cas system by preventing the SarA and ArcR-mediated activation of cas genes in S. aureus. The QS regulator, AgrA, directly binds the promoters of two transcriptional regulator encoding genes sarA and arcR to inhibit their expression. However, both SarA and ArcR act as positive regulators to promote the transcription of cas genes through direct binding a novel promoter Pcas, which was identified to locate within cas1 displays as a critical regulatory node to initiate the transcription of cas10 and csm3. Our results unveil a new regulatory mechanism for QS-mediated repression of the Type III-A CRISPR-Cas system, which may allow S. aureus to acquire foreign genetic elements encoding antibiotic resistance or virulence factors specifically at high cell density."],"repository":["ENA"],"description_synonyms":["MGC130048, CRISPR Locus, GH05739, CRISPR Loci, CAS1, CRISPR-Cas Loci, DmelCG2102, Materials, SGCG_HUMAN, Clustered Regularly Interspaced Short Palindromic Repeat, cell-cell signaling involved in quorum sensing, Crkas, Sara1, CRISPR Spacer, P120CAS, A4, Gene, CRISPR Array, SMADIP, l(2)k03902, TYPE, DAGA4, Quorum, 2610100P18Rik, MADHIP, III, GH14582, Quorum Quenchings, NSP, Quenching, 35DAG, PPP1R173, Dcas, cold antibody disease, 3, developmental verbal apraxia, EWSR1, SARFH, MAM, gamma-SG, CRKAS, p120(ctn), Sarfh, SCG3, Sara, Staphlococcus pyogenes citreus, Arrays, CRISPR Spacers, speech-language disorder-1, DmelCG2043, AA407533, gamma sarcoglycan, lcp3, Genetic, Elements, Staphilococcus aureus, speech-language disorder 1, LCP-3, CG13281, cold antibody, Micrococcus aureus, SPCH1, CG3606, pen19, CRISPR Spacer Sequence, l(3)j1C2, CRISPR Sequences, CRISPR Cluster, CRISPR, CRISPRs, DmelCG13281, CRISPR-Cas Locus, detection of cell density by secreted molecule, SARA1, ming, CP3, Capts, Loci, AI385681, E030027L17, gamma-sarcoglycan, CG7776, Staphylococus aureus, Pen19, P19, CASS1, Spacer Sequence, P130CAS, P130Cas, CRISPR Spacer Sequences, CRISPR Clusters, CRISPR Sequence, l(3)neo33, LcpIII, Sensing, 35 kDa dystrophin-associated glycoprotein, p120, anon-Pen19, cold antibody hemolytic anemia, SG-gamma, e(Pc), Xpo2, XPO2, CRISPR Arrays, das, CRISPR-Cas, Clusters, p19, BcDNA:GM09207, Cistrons, L[[3]]CP3, SGCG, LGMD2C, BcDNA:LD14270, Sequences, CTNND, CRISPR Element, p120(CTN), masra2, Sequence, anemia, CRISPR Elements, CG2102, TFIID, Genetic Materials, sarcoglycan, Cas, CAS, Madhip, Spacer, LCP3, activation, Caz, Quorum Quenching, Quenchings, developmental apraxia of speech, dSARA, DmelCG7776, Element, Staphylococcus aureus subsp. anaerobius, sara, DMDA1, DMLCP3, cold antibody hemolytic anaemia, SAR1, l(2)28-28-12, anon-35Fa, cas, 1600019H17Rik, P120CTN, gamma (35kDa dystrophin-associated glycoprotein), SARA, CG15667, Locus, hemolytic, dcas, Streptococcus aureus, p120(CAS), DmelCG5861, CSE1, DMDA, Micrococcus pyogenes, Staphylococcus pyogenes aureus, DmelCG15667, articulatory apraxia, Cluster, anon-35Fc, Material, 35kD dystrophin-associated glycoprotein, DmelLcp3, E(PC), BG:DS02740.11, anon-48Ac, SCARMD2, Spacers, DmelCG3606, Array, CRISPR Cas Loci, p120 catenin, p120(cas), Cistron, Spacer Sequences, CG2043, Genetic Material., Cadherin-associated Src substrate"],"name_synonyms":["MGC130048, CRISPR Locus, GH05739, CRISPR Loci, CAS1, CRISPR-Cas Loci, DmelCG2102, Materials, SGCG_HUMAN, Clustered Regularly Interspaced Short Palindromic Repeat, cell-cell signaling involved in quorum sensing, Crkas, Sara1, CRISPR Spacer, P120CAS, A4, Gene, CRISPR Array, SMADIP, l(2)k03902, TYPE, DAGA4, Quorum, 2610100P18Rik, MADHIP, III, GH14582, Quorum Quenchings, NSP, Quenching, 35DAG, PPP1R173, Dcas, cold antibody disease, 3, developmental verbal apraxia, EWSR1, SARFH, MAM, gamma-SG, CRKAS, p120(ctn), Sarfh, SCG3, Sara, Staphlococcus pyogenes citreus, Arrays, CRISPR Spacers, speech-language disorder-1, DmelCG2043, AA407533, gamma sarcoglycan, lcp3, Genetic, Elements, Staphilococcus aureus, speech-language disorder 1, LCP-3, CG13281, cold antibody, Micrococcus aureus, SPCH1, CG3606, pen19, CRISPR Spacer Sequence, l(3)j1C2, CRISPR Sequences, CRISPR Cluster, CRISPR, CRISPRs, DmelCG13281, CRISPR-Cas Locus, detection of cell density by secreted molecule, SARA1, ming, CP3, Capts, Loci, AI385681, E030027L17, gamma-sarcoglycan, CG7776, Staphylococus aureus, Pen19, P19, CASS1, Spacer Sequence, P130CAS, P130Cas, CRISPR Spacer Sequences, CRISPR Clusters, CRISPR Sequence, l(3)neo33, LcpIII, Sensing, 35 kDa dystrophin-associated glycoprotein, p120, anon-Pen19, cold antibody hemolytic anemia, SG-gamma, e(Pc), Xpo2, XPO2, CRISPR Arrays, das, CRISPR-Cas, Clusters, p19, BcDNA:GM09207, Cistrons, L[[3]]CP3, SGCG, LGMD2C, BcDNA:LD14270, Sequences, CTNND, CRISPR Element, p120(CTN), masra2, Sequence, anemia, CRISPR Elements, CG2102, TFIID, Genetic Materials, sarcoglycan, Cas, CAS, Madhip, Spacer, LCP3, activation, Caz, Quorum Quenching, Quenchings, developmental apraxia of speech, dSARA, DmelCG7776, Element, Staphylococcus aureus subsp. anaerobius, sara, DMDA1, DMLCP3, cold antibody hemolytic anaemia, SAR1, l(2)28-28-12, anon-35Fa, cas, 1600019H17Rik, P120CTN, gamma (35kDa dystrophin-associated glycoprotein), SARA, CG15667, Locus, hemolytic, dcas, Streptococcus aureus, p120(CAS), DmelCG5861, CSE1, DMDA, Micrococcus pyogenes, Staphylococcus pyogenes aureus, DmelCG15667, articulatory apraxia, Cluster, anon-35Fc, Material, 35kD dystrophin-associated glycoprotein, DmelLcp3, E(PC), BG:DS02740.11, anon-48Ac, SCARMD2, Spacers, DmelCG3606, Array, CRISPR Cas Loci, p120 catenin, p120(cas), Cistron, Spacer Sequences, CG2043, Genetic Material., Cadherin-associated Src substrate"],"additional_accession":[]},"is_claimable":false,"name":"Quorum sensing represses Type III-A CRISPR-Cas in Staphylococcus aureus by inhibiting SarA and ArcR-mediated activation of cas genes","description":"Quorum sensing represses Type III-A CRISPR-Cas in Staphylococcus aureus by inhibiting SarA and ArcR-mediated activation of cas genes","dates":{"last_updated":"2022-12-01","first_public":"2022-12-01"},"accession":"PRJEB52726","cross_references":{}}