{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR103/018/ERR10390818/ERR10390818_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR103/018/ERR10390818/ERR10390818_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["European Bioinformatics Institute","Karolinska Institutet, Scilifelab"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB56887"],"broker_name":["ArrayExpress"],"long_description":["To study cancer cells heterogeneity at the single cell level we grew cancer cells as spheroids and extracted their RNA preform SmartSeq3xpress. We grew MDA-MB-231 cells on agar coated plates for 5-10 days in DMEM 10% FBS. The spheroids were incubated for 2 hours with Calcein AM and Vybrant Dye 10uM at 37C and washed twice with PBS. After dissociation with trypsinLE 0.25% the cells were facs sorted and the fluorescence intensity for each cell was recorded. The RNA were extracted and the cDNA libraries were built according to the SmartSeq3xpress protocol."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Single cell RNAseq on MDA-MB-231 spheroids and in co-culture with THP1","description":"Single cell RNAseq on MDA-MB-231 spheroids and in co-culture with THP1","dates":{"last_updated":"2023-10-10","first_public":"2023-10-10"},"accession":"PRJEB56887","cross_references":{}}