ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR103/018/ERR10390818/ERR10390818_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR103/018/ERR10390818/ERR10390818_1.fastq.gzprimary200OKGenomicsEuropean Bioinformatics InstituteKarolinska Institutet, Scilifelabhttps://www.ebi.ac.uk/ena/browser/view/PRJEB56887ArrayExpressTo study cancer cells heterogeneity at the single cell level we grew cancer cells as spheroids and extracted their RNA preform SmartSeq3xpress. We grew MDA-MB-231 cells on agar coated plates for 5-10 days in DMEM 10% FBS. The spheroids were incubated for 2 hours with Calcein AM and Vybrant Dye 10uM at 37C and washed twice with PBS. After dissociation with trypsinLE 0.25% the cells were facs sorted and the fluorescence intensity for each cell was recorded. The RNA were extracted and the cDNA libraries were built according to the SmartSeq3xpress protocol.ENAfalseSingle cell RNAseq on MDA-MB-231 spheroids and in co-culture with THP1Single cell RNAseq on MDA-MB-231 spheroids and in co-culture with THP12023-10-102023-10-10PRJEB56887