ENAapplication/xmlftp://primary200OKGenomicsMolecular Genetics, Technical University Darmstadthttps://www.ebi.ac.uk/ena/browser/view/PRJEB60364xref:PubMed:36980882Nanopore direct RNA sequencing was performed on culture-derived cells of the bloodstream and insect life cycle stages of Trypanosoma brucei. Novel isoforms of annotated transcripts as well as novel non-coding RNAs were identified. 10% of the putative lncRNAs are differentially expressed, the majority being up-regulated in bloodstream cells. In addition, differential gene expression analysis based on long reads confirmed the stage-regulated genes identified by other techniques.ENAfalseNanopore DRS of Trypanosoma bruceiNanopore-based direct RNA sequencing of the Trypanosoma brucei transcriptome2023-04-202023-03-09PRJEB6036436980882