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rechts der Isar (TUM)University Medicine Goettingenhttps://www.ebi.ac.uk/ena/browser/view/PRJEB63203Described experiments are published in https://doi.org/10.1002/advs.202307695. Two murine PDAC cell lines were transduced with pLenti-PGK-puro-Prkaa1 and empty control plasmids to generate Prkaa1 overexpressing cell lines (8182_PGK_empty vs. 8182_PGK_Prkaa1, 9091_PGK_empty vs. 9091_PGK_Prkaa1, Addgene #19068 and #204356). Furthermore, three murine PDAC cell lines were used to generate Prkaa1 KO cell lines. For each cell line, one control plasmid (LacZ) and two different sgRNAs targeting Prkaa1 were used (8570_LacZ vs. 8570_Prkaa1_KO1 vs. 8570_Prkaa1_KO2, 9091_LacZ vs. 9091_Prkaa1_KO1 vs. 9091_Prkaa1_KO2, 8248_LacZ vs. 8248_Prkaa1_KO1 vs. 8248_Prkaa1_KO2, doi: 10.1038/s43018-021-00326-1). Finally, two murine PDAC cell lines were treated with PF-3758309 for 24 hours (8182_con vs. 8182_PF3758309, 9091_con vs. 9091_PF3758309). Parental murine cell lines (9091, 8248, 8182, 8570) which were used for the described experiments are first described in https://doi.org/10.1038/nature25459.xref:EuropePMC:PMC11336956ENAAI450832, ampk2, treatment, Therapy, AMPKalpha1, PF-3758309, disease management., AI194361, C130083N04Rik, Therapeutic, ampk, AL024255, ampka1, PF3758309, Therapies, Treatment, prkaa, AMPK, AMPKa1, Treatments, prkaa1AI450832, pancreatic ductal carcinoma., ampk2, AMPKalpha1, AI194361, C130083N04Rik, ampk, AL024255, ampka1, PDAC, prkaa, AMPK, AMPKa1, prkaa1falsePrkaa1 in PDACPrkaa1 Overexpression, KO models, and PF-3758309 treatment2024-06-192024-06-19PRJEB63203