ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/018/ERR12654318/ERR12654318_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/030/ERR12654330/ERR12654330_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/026/ERR12654326/ERR12654326_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/034/ERR12654334/ERR12654334_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/037/ERR12654337/ERR12654337_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/017/ERR12654317/ERR12654317_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/016/ERR12654316/ERR12654316_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/032/ERR12654332/ERR12654332_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/025/ERR12654325/ERR12654325_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/020/ERR12654320/ERR12654320_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/029/ERR12654329/ERR12654329_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/017/ERR12654317/ERR12654317_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/033/ERR12654333/ERR12654333_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/023/ERR12654323/ERR12654323_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/027/ERR12654327/ERR12654327_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/021/ERR12654321/ERR12654321_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/028/ERR12654328/ERR12654328_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/029/ERR12654329/ERR12654329_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/034/ERR12654334/ERR12654334_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/035/ERR12654335/ERR12654335_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/015/ERR12654315/ERR12654315_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/031/ERR12654331/ERR12654331_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/024/ERR12654324/ERR12654324_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/016/ERR12654316/ERR12654316_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/020/ERR12654320/ERR12654320_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/032/ERR12654332/ERR12654332_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/023/ERR12654323/ERR12654323_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/022/ERR12654322/ERR12654322_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/019/ERR12654319/ERR12654319_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/026/ERR12654326/ERR12654326_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/036/ERR12654336/ERR12654336_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/035/ERR12654335/ERR12654335_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/031/ERR12654331/ERR12654331_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/025/ERR12654325/ERR12654325_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/019/ERR12654319/ERR12654319_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/037/ERR12654337/ERR12654337_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/030/ERR12654330/ERR12654330_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/024/ERR12654324/ERR12654324_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/015/ERR12654315/ERR12654315_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/018/ERR12654318/ERR12654318_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/033/ERR12654333/ERR12654333_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/036/ERR12654336/ERR12654336_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/028/ERR12654328/ERR12654328_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/027/ERR12654327/ERR12654327_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/021/ERR12654321/ERR12654321_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/022/ERR12654322/ERR12654322_2.fastq.gzprimaryOK200GenomicsSchool of Medicine, University of NottinghamEuropean Bioinformatics Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJEB72641ArrayExpressColorectal cancer (CRC) cell lines (LS1034 and HCT116, representing different genetic backgrounds of CRC) were co-cultured with iPSC-derived intestinal stroma (described in other protocols). Control conditions were each cell line cultured separately. A dose response for HCT116 on stroma was tested using a varying seeding density. After culture, cells were separated using MACS based on EpCAM expression (EpCAM+: CRC cell line / EpCAM-: stroma) and then measured for reciprocal changes in gene expression by RNAseq (i.e. CRC effect on stromal expression and vice verse on cell lines).ENAfalseRNAseq of colorectal cancer cell lines (LS1034/HCT116) cultured with iPSC-derived intestinal stroma, separated by EpCAM expression (MACS). Monocultures of each cell line and co-cultures with LS1034 or HCT116 with stroma.RNAseq of colorectal cancer cell lines (LS1034/HCT116) cultured with iPSC-derived intestinal stroma, separated by EpCAM expression (MACS). Monocultures of each cell line and co-cultures with LS1034 or HCT116 with stroma.2025-02-022025-02-02PRJEB72641