ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/025/ERR12687725/ERR12687725_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/025/ERR12687725/ERR12687725_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/022/ERR12687722/ERR12687722_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/023/ERR12687723/ERR12687723_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/020/ERR12687720/ERR12687720_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/024/ERR12687724/ERR12687724_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/022/ERR12687722/ERR12687722_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/021/ERR12687721/ERR12687721_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/021/ERR12687721/ERR12687721_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/024/ERR12687724/ERR12687724_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/023/ERR12687723/ERR12687723_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR126/020/ERR12687720/ERR12687720_1.fastq.gzprimaryOK200GenomicsEuropean Bioinformatics InstituteHead of Drug Discovery and Evaluation Charles University in Praguehttps://www.ebi.ac.uk/ena/browser/view/PRJEB72886ArrayExpressBenzoxaboroles (BoBs) feature a boron-heterocyclic core and are an important recent innovation in the development of drugs against a range of pathogens and other pathologies. A broad spectrum of pharmacology is associated with chemically diverse BoB derivatives and includes multiple modes-of-action and targets. However, a consensus MoA for BoBs targeting evolutionarily diverse protozoan pathogens has emerged with the identification of CPSF3/CPSF73 in the CPSF complex in both apicomplexan and kinetoplastida parasites. We have detected a functional connection between protein sumoylation and the BoB boron-heterocyclic scaffold using comprehensive genetic screens in Trypanosoma brucei. Strikingly, as part of this sumoylation response, members of the CPSF complex are specifically and rapidly destabilised in a SUMO and proteosome-dependent manner. Here we deposit RNAseq data quantifying the effects of the aminomethyl-benzoxaborole AN3057 exposure on the transcriptome landscape in T. brucei. Specifically, T. brucei bloodstream-form cells in logarithmic growth phase were treated with 400 nm AN3057 (3 × EC50 determined after 24h) for 20 min (T20) and 60 min (T60), respectively. Nontreated control cells were prepared in parallel. All samples were in 2 biological replicates.ENAfalseTrypanosoma brucei transcriptome changes upon exposure to the aminomethyl-benzoxaborole AN3057Trypanosoma brucei transcriptome changes upon exposure to the aminomethyl-benzoxaborole AN30572024-03-082024-03-08PRJEB72886