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Bioinformatics InstituteCancer Research Horizonshttps://www.ebi.ac.uk/ena/browser/view/PRJEB74454ArrayExpressThis project aims to compare gRNA design algorithms and single- versus dual-targeting using a benchmark CRISPR-knockout library composed of gRNAs targeting essential and non-essential genes.ENAfalseAmplicon DNA-sequencing of time series of pooled-CRISPR knock-out screens of cell lines using a dual-guide CRISPR libraryAmplicon DNA-sequencing of time series of pooled-CRISPR knock-out screens of cell lines using a dual-guide CRISPR library2025-02-012025-02-01PRJEB74454