ENAGenomicsEuropean Bioinformatics InstituteDepartment of Pathology, University of Cambridgehttps://www.ebi.ac.uk/ena/browser/view/PRJEB75792ArrayExpressTo understand the response of human Hofbauer cells to Listeria monocytogenes infection and how it differs between first trimester and term, we established an infection assay using primary human first-trimester and term Hofbauer cells isolated by fluorescence-activated cell sorting and cultured in vitro for 7 days. We then profiled and compared the transcriptomes of control cells (treated with PBS or Heat-Killed Listeria monocytogenes) and cells infected with Listeria monocytogenes for 8 hours using the NEBNext® Ultra II Directional RNA Library Prep Kit (Illumina).ENAfalseBulk RNA-seq profiling the response of human first-trimester and term Hofbauer cells to Listeria monocytogenes infection in vitroBulk RNA-seq profiling the response of human first-trimester and term Hofbauer cells to Listeria monocytogenes infection in vitro2025-08-052025-08-05PRJEB75792