{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/074/ERR13231274/ERR13231274_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/075/ERR13231275/ERR13231275_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/078/ERR13231278/ERR13231278_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/079/ERR13231279/ERR13231279_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/076/ERR13231276/ERR13231276_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/077/ERR13231277/ERR13231277_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/074/ERR13231274/ERR13231274_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/077/ERR13231277/ERR13231277_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/079/ERR13231279/ERR13231279_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/078/ERR13231278/ERR13231278_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/076/ERR13231276/ERR13231276_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR132/075/ERR13231275/ERR13231275_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["universita degli studi di verona"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB76303"],"long_description":["Previous studies in sports science suggested that regular exercise has a positive impact on human health. However, the effects of endurance sports and their underlying mechanisms are still not com-pletely understood. One of the main debates regards the modulation of immune dynamics in high-intensity exercise. As part of the \"Run 4 Science\" project in Verona, Italy, we conducted a single-cell RNA sequencing analysis on half-marathon amateur runners to investigate the transcriptional dynam-ics of peripheral blood mononuclear cells following endurance exercise. Blood samples were collected from 4 participants before and after running a half-marathon to carry out a comprehensive tran-scriptomic analysis of immune cells at the single-cell level. Our analysis revealed significant altera-tions in the transcriptional profiles following endurance physical exercise. Modulations in myeloid cells suggested the activation of stress response (6 related pathways, p-value < 0.04) and pathways related to viral processes (4 related pathways, p-value < 0.03), while in lymphoid cells they hinted to a shift towards immune activation (24 related pathways, p-value < 0.01). Additionally, transcriptional changes in platelets point to an activation of the coagulation process (5 related pathways, p-value < 0.005). Single-cell data was also analysed following a pseudo-bulk approach (i.e., simulating a bulk RNAseq experiment) to gain further biological insights. Our findings suggest that a pseudo-bulk analysis could offer complementary findings to classical single-cell analysis methods and demonstrate that endurance physical exercise, such as running a half-marathon, induces substantial changes in the transcriptional dynamics of immune cells. These insights contribute to a better understanding of the immune modulation mediated by endurance exercise and may inform future training routines or nutri-tional guidelines based on individual gene expression levels."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing analysis reveals exercise-induced transcriptional dynamics in half-marathon runners","description":"Single-cell RNA sequencing analysis reveals exercise-induced transcriptional dynamics in half-marathon runners","dates":{"last_updated":"2024-09-16","first_public":"2024-09-16"},"accession":"PRJEB76303","cross_references":{}}