ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/015/ERR14878415/ERR14878415.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR133/081/ERR13361481/ERR13361481.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/026/ERR14878426/ERR14878426.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/024/ERR14878424/ERR14878424.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/030/ERR14878430/ERR14878430.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/013/ERR14878413/ERR14878413.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/023/ERR14878423/ERR14878423.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/028/ERR14878428/ERR14878428.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/021/ERR14878421/ERR14878421.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/017/ERR14878417/ERR14878417.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/022/ERR14878422/ERR14878422.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/019/ERR14878419/ERR14878419.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/014/ERR14878414/ERR14878414.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR133/080/ERR13361480/ERR13361480.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/025/ERR14878425/ERR14878425.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/027/ERR14878427/ERR14878427.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR133/082/ERR13361482/ERR13361482.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/016/ERR14878416/ERR14878416.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/018/ERR14878418/ERR14878418.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/020/ERR14878420/ERR14878420.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR148/029/ERR14878429/ERR14878429.fastq.gzprimaryOK200GenomicsThe Ohio State Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJEB77080We present universal reverse transcription methods (UmRT and UmRT+) that enhance Nanopore direct RNA sequencing of long and structured RNAs. These protocols enable high-efficiency, full-length recovery of challenging transcripts—including the 20 Kb XIST RNA—and resolve isoform-specific expression, m6A modifications, and poly(A) tail variation. This advance enables high-resolution profiling of native RNAs, with broad utility in transcriptomics, virology, and RNA-based diagnostics.ENAfalseUniversal RNA Linearization Enables Full-length Nanopore DRS Across Whole TranscriptomesNanopore sequencing data for "Universal RNA Linearization Enables Full-length Nanopore DRS Across Whole Transcriptomes" paper2025-05-292025-05-29PRJEB77080