{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB80123"],"long_description":["Red blood cell development is a unique process where greatly reduced transcriptome and proteome complexity facilitates vast hemoglobin production. Here, we describe the cooperative role of cytoplasmic poly(A) polymerase TENT5C and the poly(A) tail-protecting LARP4/5 RNA-binding proteins in ensuring proper hemoglobin production. To investigate the role of Tent5c and Larp4/5 in poly(A) tail regulation in terminal erythropoiesis, we generated Nanopore direct RNA sequencing (DRS) libraries from RNA isolated from murine fetal liver erythroblasts in fetal age 14.5 day in WT and TENT5C KO mice. Libraries were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the Flow Cell Type FLO-MIN102 (R9.4.1) with the MinION (ONT) device controlled by MinKNOW (ONT) software. Obtained reads were basecalled using Guppy (ONT). Quality and fragment size distribution of the libraries was determined by electrophoresis on Agilent 2100 Bioanalyzer (Agilent Technologies Inc.)."],"repository":["ENA"],"description_synonyms":["Policy, Economic Policy, DXErtd793., Policies, polynuclear neutrophilic leukocyte, Home Economics, Indices, Index, Conditions, Hypothesis, Utility, Factor, Production, Living Cost, erythropoiesis, Macroeconomic Factors, Consumption, Economics, Easterlin, neutrocyte, Economic Condition, Generic Action, Household, neutrophilic leucocyte, Consumer Price Index, Consumer Price, Utility Theory, neutrophil leukocyte, erythrocyte cell differentiation, Macroeconomic, hemerythrin, Microeconomic, Action, Theory, Utility Theories, Factors, red blood cell differentiation, DXErtd793e, Household Consumption, Erythropoieses, neutrophil leucocyte, Consumer Price Indices, Economic Policies, Microeconomic Factors, Living Costs, neutrophilic leukocyte, Economic Conditions, Home, oxygen-carrying, RBC differentiation, Condition, Easterlin Hypothesis, Remittance, Capital, Cost of Living, polymorphonuclear leukocyte, polynuclear neutrophilic leucocyte, Globin, PMN, Household Consumptions, polymorphonuclear leucocyte, Economic, globin, Theories, hemocyanin, poly, polymorphonuclear neutrophil, Remittances"],"name_synonyms":["Policy, Economic Policy, DXErtd793., Policies, polynuclear neutrophilic leukocyte, Home Economics, Indices, Index, Conditions, Hypothesis, Utility, Factor, Production, Living Cost, erythropoiesis, Macroeconomic Factors, Consumption, Economics, Easterlin, neutrocyte, Economic Condition, Generic Action, Household, neutrophilic leucocyte, Consumer Price Index, Consumer Price, Utility Theory, neutrophil leukocyte, erythrocyte cell differentiation, Macroeconomic, hemerythrin, Microeconomic, Action, Theory, Utility Theories, Factors, red blood cell differentiation, DXErtd793e, Household Consumption, Erythropoieses, neutrophil leucocyte, Consumer Price Indices, Economic Policies, Microeconomic Factors, Living Costs, neutrophilic leukocyte, Economic Conditions, Home, oxygen-carrying, RBC differentiation, Condition, Easterlin Hypothesis, Remittance, Capital, Cost of Living, polymorphonuclear leukocyte, polynuclear neutrophilic leucocyte, Globin, PMN, Household Consumptions, polymorphonuclear leucocyte, Economic, globin, Theories, hemocyanin, poly, polymorphonuclear neutrophil, Remittances"],"additional_accession":[]},"is_claimable":false,"name":"Efficient globin production during terminal erythropoiesis depends on the synergistic action of TENT5C poly(A) polymerase and LARP4/5","description":"Efficient globin production during terminal erythropoiesis depends on the synergistic action of TENT5C poly(A) polymerase and LARP4/5","dates":{"last_updated":"2024-11-14","first_public":"2024-11-14"},"accession":"PRJEB80123","cross_references":{}}