{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["INRAE","Institut National de Recherche pour L'agriculture, l'alimentation et l'Environnement"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB83122"],"long_description":["Pectin is a complex plant heteropolysaccharide whose structure and function differ depending on its source. In animal feed, breaking down pectin is essential, as its presence increases feed viscosity and reduces nutrient absorption. Soybean meal, a protein-rich poultry feed ingredient, contains significant amounts of pectin, the structure of which remains unclear. Consequently, the enzyme activities required to degrade soybean meal pectin and how they interact are still open questions. In this study, we produced 15 recombinant fungal carbohydrate-active enzymes (CAZymes) identified from fungal secretomes acting on pectin. After observing that these enzymes were not active on soybean meal pectin when used alone, we developed a semi miniaturized method to evaluate their effect as multi-activity cocktails. We designed and tested 12 enzyme pools, containing up to 15 different CAZymes, using several hydrolysis markers. Thanks to our multi activity enzymatic approach combined with a Pearson correlation matrix, we identified 10 fungal CAZymes efficient on soybean meal pectin, 9 of which originate from Talaromyces versatilis. Based on enzyme specificity and linkage analysis, we propose a structural model for soybean meal pectin. Our findings underscore the importance of combining CAZymes to improve the degradation of agricultural co-products."],"repository":["ENA"],"description_synonyms":["Rice Meals, Semolina Flours, biochemical pathways, single-organism catabolic process, multicellular organismal catabolic process, cellular breakdown, Almond Flours, Corn Meals, Almond Flour, degradation, Pectinic Acid, Grain Meals, Biocatalysts, Almond Meals, Almond Meal, Nut Flour, Corn Flour, Semolina Flour, Methoxypectin, Potato, Flours, Methoxylpectin, Corn Meal, enzyme activity, Rice Flour, Rice Flours, Meals, cellular catabolism, Wheat Flours, Grain Flour, Semolina, Soybean Flours, Methoxy, Pectin, Flour, Corn Flours, Soybean Meal, Nut Flours, Zinc Pectinate, cellular degradation, Almond, Wheat Meal, secretion, Methoxy Pectin, Rice Meal, Grain Meal, Wheat, Soybean Meals, Calcium, Grain, Meal, breakdown, enzymes, biodegradation, breakdown of molecule., Soybean, breakdown of chemical, catabolism, Grain Flours, Wheat Flour, Rice, breakdown of substance, Enzyme, Pectinate, Corn, Potato Flour, Nut, Soybean Flour, Wheat Meals, Zinc, Calcium Pectinate, Biocatalyst, biotransformation, Potato Flours"],"name_synonyms":["Rice Meals, Semolina Flours, Almond Flours, Corn Meals, Almond Flour, Grain Meals, Pectinic Acid, Almond Meals, Almond Meal, Biocatalysts, Nut Flour, Corn Flour, Semolina Flour, Potato, Methoxypectin, Flours, Corn Meal, Methoxylpectin, Rice Flour, Rice Flours, Meals, Wheat Flours, Grain Flour, Semolina, Soybean Flours, Methoxy, Pectin, Flour, Corn Flours, Soybean Meal, Nut Flours, Zinc Pectinate, Almond, Wheat Meal, Methoxy Pectin, Rice Meal, Grain Meal, Wheat, Soybean Meals, Calcium, Grain, Meal, Soybean, Grain Flours, Biocatalyst., Wheat Flour, Rice, Enzyme, Corn, Pectinate, Potato Flour, Nut, Soybean Flour, Wheat Meals, Zinc, Calcium Pectinate, Potato Flours"],"additional_accession":[]},"is_claimable":false,"name":"Unlocking soybean meal pectin recalcitrance using a multi enzyme cocktail approach","description":"Fungal pectin-degrading enzymes for soybean meal degradation","dates":{"last_updated":"2024-12-08","first_public":"2024-12-08"},"accession":"PRJEB83122","cross_references":{}}