{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Korea Institute of Radiological & Medical Sciences","KIRAMS"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB87508"],"long_description":["An elevated extracellular matrix (ECM) and interstitial fluid pressure (IFP) in gastric cancer limits the targeting of HER2-expressing GC cells when radioimmunotherapy (RIT) with 64Cu-trastuzumab (64Cu-TRZ) is utilized. Here, we used Losartan (LOS) to downregulate ECM and IFP in gastric cancer mice model. In our study we treated the gastric cancer mice model with a dose of 40 mg/kg of LOS. We found that the LOS treatment increases a 2-fold higher Alexa-647-TRZ accumulation which significantly enhanced 64Cu-TRZ. We determined that the LOS-treated samples exhibited reduced mRNA and protein expression of SERPINE1, a gene associated with the ECM degradation. Additionally, LOS treatment resulted in the downregulated mRNA expression of the TGF-β1 and COL13A1, the genes involved in ECM deposition and an upregulated RNA expression of MMP2, a gene associated with the ECM degradation. There were no significant changes in metastatic markers of N-Cadherin and E-Cadherin. Moreover, our study demonstrates that silencing SERPINE1 increases the activity of the MMP2 and decreases COL13A1 with no effect on the N-cadherin and E-cadherin were observed. Our novel combinational therapy of using 64Cu-TRZ with LOS is attributed to the downregulation of SERPINE1 targeting ECM and IFP is highly effective for treatment of gastric cancer."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Tumor microenvironment modulation by SERPINE1 increases radioimmunotherapy in murine model of gastric cancer","description":"SERPINE1-Mediated Tumor Microenvironment Modulation Enhances Radioimmunotherapy in a Murine Gastric Cancer Model","dates":{"last_updated":"2025-03-24","first_public":"2025-03-24"},"accession":"PRJEB87508","cross_references":{}}