ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/051/ERR15006951/ERR15006951_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/053/ERR15006953/ERR15006953_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/054/ERR15006954/ERR15006954_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/055/ERR15006955/ERR15006955_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/053/ERR15006953/ERR15006953_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/052/ERR15006952/ERR15006952_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/051/ERR15006951/ERR15006951_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/052/ERR15006952/ERR15006952_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/054/ERR15006954/ERR15006954_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/055/ERR15006955/ERR15006955_2.fastq.gzprimary200OKGenomicsPhilipps University Marburghttps://www.ebi.ac.uk/ena/browser/view/PRJEB89807A fusion of the HNH domain of CasDinG from Sulfitobacter sp. JL08 (SsCasDinG) to the characterized type IV-A1 CasDinG of Pseudomonas oleovorans (PoCasDinG) was created. An engineered catalytic active type IV-A1 CRISPR-Cas system able to processive degrade target DNA in vivo. Using whole genome sequencing we analyzed the effects on the genome reduction in E. coli BL21-AI also in comparison to the type I-Fv system of Shewanella putrefaciens in association with its CasDinG. This study provides the basis to use the type I-Fv and the engineered type IV-A1 systems as tools in genome reduction and helps to understand the DNA repair mechanisms used to fix CRISPR-Cas induced lesions.ENAfalseEngineered Type IV-A and Type I-Fv CRISPR-Cas effectors function in reduction of the Escherichia coli genomeType IV-A and Type I-Fv application to genome reduction2025-05-272025-05-27PRJEB89807