ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/074/ERR15009374/ERR15009374_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/082/ERR15009382/ERR15009382_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/078/ERR15009378/ERR15009378_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/085/ERR15009385/ERR15009385_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/086/ERR15009386/ERR15009386_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/080/ERR15009380/ERR15009380_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/073/ERR15009373/ERR15009373_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/068/ERR15009368/ERR15009368_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/077/ERR15009377/ERR15009377_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/087/ERR15009387/ERR15009387_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/081/ERR15009381/ERR15009381_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/083/ERR15009383/ERR15009383_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/069/ERR15009369/ERR15009369_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/075/ERR15009375/ERR15009375_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/077/ERR15009377/ERR15009377_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/076/ERR15009376/ERR15009376_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/071/ERR15009371/ERR15009371_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/082/ERR15009382/ERR15009382_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/080/ERR15009380/ERR15009380_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/076/ERR15009376/ERR15009376_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/072/ERR15009372/ERR15009372_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/068/ERR15009368/ERR15009368_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/087/ERR15009387/ERR15009387_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/079/ERR15009379/ERR15009379_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/070/ERR15009370/ERR15009370_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/074/ERR15009374/ERR15009374_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/067/ERR15009367/ERR15009367_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/071/ERR15009371/ERR15009371_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/086/ERR15009386/ERR15009386_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/083/ERR15009383/ERR15009383_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/084/ERR15009384/ERR15009384_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/067/ERR15009367/ERR15009367_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/073/ERR15009373/ERR15009373_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/085/ERR15009385/ERR15009385_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/075/ERR15009375/ERR15009375_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/072/ERR15009372/ERR15009372_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/084/ERR15009384/ERR15009384_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/081/ERR15009381/ERR15009381_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/079/ERR15009379/ERR15009379_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/078/ERR15009378/ERR15009378_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/070/ERR15009370/ERR15009370_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR150/069/ERR15009369/ERR15009369_1.fastq.gzprimaryOK200GenomicsNational Centre for Scientific ResearchCNRShttps://www.ebi.ac.uk/ena/browser/view/PRJEB89852Arbuscular mycorrhiza (AM) with soilborne Glomeromycota fungi was pivotal in the conquest of land by plants almost half a billion years ago. In flowering plants, it is hypothesized that AM is initiated by the perception of AM fungi-derived chito- and lipochito-oligosaccharides (COs/LCOs) in the host via Lysin Motif Receptor-Like Kinases (LysM-RLKs). However, it remains uncertain whether plant perception of these molecules is a prerequisite for AM establishment and for its origin. Here, we made use of the reduced LysM-RLK complement present in the liverwort Marchantia paleacea to assess the conservation of the role played by this class of receptors during AM and in CO/LCO perception. Our reverse genetic approach demonstrates the critical function of a single LysM-RLK, MpaLYKa, in AM formation, thereby supporting an ancestral function for this receptor in symbiosis. Binding studies, cytosolic calcium variation recordings and genome-wide transcriptomics indicate that another LysM-RLK of M. paleacea, MpaLYR, is also required for triggering a response to COs and tested LCOs, despite being dispensable for AM formation. Collectively, our results demonstrate that the perception of symbionts by LysM-RLK is an ancestral feature in land plants, and suggest the existence of yet-uncharacterized AM fungi signals.ENAfalseA plant Lysin Motif Receptor-Like Kinase plays an ancestral function in mycorrhizaFunctions of plant Lysin Motif Receptor-Like Kinase2025-05-232025-05-23PRJEB89852