{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["European Bioinformatics Institute","Department of Infectious Diseases, Imperial College London"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJEB95820"],"broker_name":["ArrayExpress"],"long_description":["RNA-seq was carried out to compare the transcriptomes of wild-type MG1655 E coli with mutants lacking the glucose metabolism regulator TmaR , in bacteria that had been exposed to short-term (3hr) and long-term (24hr) nitrogen starvation in Gutnick minimal media. A rifampicin-chase experiment was performed following long-term (24hr) nitrogen starvation, with 150ug/ml rifampicin added at N-24, with samples taken 15 and 60 minutes following treatment. TmaR is required for the formation of Hfq foci, a novel sub-cellular feature that forms during long-term nitrogen starvation. The aim of this work was to use the mutant lacking TmaR to understand the functional role of the Hfq foci, and whether they play a role in sRNA stability during long-term nitrogen starvation."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq analysis of MG1655 Escherichia coli (Wild-type, and mutants lacking the glucose metabolism regulator TmaR) experiencing short- & long-term N starvation and following treatment with rifampicin during long-term N starvation in Gutnick minimal media","description":"RNA-seq analysis of MG1655 Escherichia coli (Wild-type, and mutants lacking the glucose metabolism regulator TmaR) experiencing short- & long-term N starvation and following treatment with rifampicin during long-term N starvation in Gutnick minimal media","dates":{"last_updated":"2025-09-23","first_public":"2025-09-23"},"accession":"PRJEB95820","cross_references":{}}