ENAGenomicsThe Ohio State Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1013281The isolation of Bacillus proteolyticus OSUB18 from switchgrass has unveiled its significant potential in both the enhancement of plant growth and the suppression of plant diseases. The elucidation of mechanisms governing this intricate plant-microbe interaction involved the utilization of the model plant Arabidopsis thaliana. Notably, OSUB18 treatment has been observed to foster the growth of Arabidopsis plants under diverse abiotic stress conditions. Employing advanced RNA-seq technology, this study meticulously investigated differentially expressed genes within Arabidopsis plants due to OSUB18 inoculation. Remarkably, our phenotypic findings closely align with the outcomes derived from RNA-seq and Metascape analysis. Moreover, the introduction of OSUB18 through local infiltration modulates the plant's systemic immune response, thereby priming Arabidopsis plants to effectively counteract phytopathogens with varying lifestyles. Furthermore, OSUB18 exhibits pronounced antagonistic effects against a range of phytopathogens, encompassing bacteria, fungi, and oomycetes, as demonstrated in vitro. In summation, our findings substantiate that OSUB18 exerts a stimulatory influence on plant growth, potentially attributed to the remodelling of root architecture and the comprehensive mitigation of various plant diseases. This dual effect is achieved through both direct antagonism and the indirect priming of host systemic resistance, as corroborated by our comprehensive phenotypic observations and transcriptome analysis.xref:EuropePMC:PMC10740446ENAfalsePlant growth promotion and plant disease suppression through inoculation with Bacillus proteolyticus OSUB182024-07-242024-05-11PRJNA1013281