ENAGenomicsCentral Institute for Research on Buffaloeshttps://www.ebi.ac.uk/ena/browser/view/PRJNA1027138Bubaline alphaherpesvirus 1In June 2021, a disease suspected of Herpes virus infection with clinical manifestation of diffused pustular vulvovaginitis was reported in a 2 years old Murrah buffalo heifer in the Chhani Badi village, Hanumangarh, Rajasthan of India. The herd consisted of five animals (2 lactating buffaloes, 1 heifer and 2 calves). Other animals of herd were healthy and without any clinical signs. The inspected heifer recovered after a few days. In order to identify the causative agent, vaginal lesion sample using vaginal swab was subjected to initial screening by PCR . Following PCR confirmation, the virus was isolated from the vaginal swab inducing a cytopathic effect in MDBK cells. The final identification of the virus was done by sequence analysis.pathogenpathogen:virusENAvirusesfalseBubaline alphaherpesvirus 1 isolate:HeiferVagina-S102_1Bubaline alphaherpesvirus 1 isolate:HeiferVagina-S102_1 Raw sequence reads2023-10-142023-10-14PRJNA1027138202910