{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/091/SRR26501891/SRR26501891_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/090/SRR26501890/SRR26501890_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/086/SRR26501886/SRR26501886_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/088/SRR26501888/SRR26501888_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/086/SRR26501886/SRR26501886_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/087/SRR26501887/SRR26501887_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/089/SRR26501889/SRR26501889_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/090/SRR26501890/SRR26501890_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/087/SRR26501887/SRR26501887_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/091/SRR26501891/SRR26501891_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/089/SRR26501889/SRR26501889_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/088/SRR26501888/SRR26501888_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["MaIAGE, INRAE - Université Paris-Saclay"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1031675"],"scientific_name":["Listeria monocytogenes EGD-e"],"long_description":["Bacteria have developed multiple strategies, such as sporulation, to cope with environmental stress. Non-sporulating bacteria, however, may “hibernate” into a so-called viable but non-culturable (VBNC) state, where they are no longer able to grow in standard culture media and thus become undetectable by conventional growth-based methods. VBNC pathogens pose a significant risk for human and animal health as they can “wake up” back into a vegetative and virulent state. Although hundreds of bacterial species have been reported to enter a VBNC state in response to various stresses (e.g. thermal, osmotic, starvation, antibiotics), the molecular mechanisms governing this phenotypic switch remains largely elusive. Here, we report an in-depth characterization of the VBNC state transition process in the bacterial pathogen Listeria monocytogenes in response to nutritional deprivation. We found that starvation in mineral water drives L.monocytogenes into a VBNC state via a unique mechanism of cell wall shedding that generates cellwall-deficient coccoid forms. Transcriptomic and gene-targeted approaches revealed the stress response regulator SigB and the autolysin NamA as major mediators of cell wall loss and VBNC state transition. Overall design: Comparison between cells in the first day and after 7 days of incubation in mineral water, with biological triplicates."],"tag":["pathogen:bacterium","pathogen","xref:PubMed:39358320"],"repository":["ENA"],"classification":["bacteria"],"additional_accession":[]},"is_claimable":false,"name":"Deep starvation induces loss of cell wall and dormancy in Listeria","description":"Deep starvation induces loss of cell wall and dormancy in Listeria","dates":{"last_updated":"2025-09-24","first_public":"2024-08-03"},"accession":"PRJNA1031675","cross_references":{"GEO":["GSE246157"],"taxon":["169963"],"PubMed":["39358320"]}}