ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/091/SRR26501891/SRR26501891_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/090/SRR26501890/SRR26501890_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/086/SRR26501886/SRR26501886_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/088/SRR26501888/SRR26501888_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/086/SRR26501886/SRR26501886_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/087/SRR26501887/SRR26501887_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/089/SRR26501889/SRR26501889_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/090/SRR26501890/SRR26501890_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/087/SRR26501887/SRR26501887_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/091/SRR26501891/SRR26501891_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/089/SRR26501889/SRR26501889_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR265/088/SRR26501888/SRR26501888_1.fastq.gzprimaryOK200GenomicsMaIAGE, INRAE - Université Paris-Saclayhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1031675Listeria monocytogenes EGD-eBacteria have developed multiple strategies, such as sporulation, to cope with environmental stress. Non-sporulating bacteria, however, may “hibernate” into a so-called viable but non-culturable (VBNC) state, where they are no longer able to grow in standard culture media and thus become undetectable by conventional growth-based methods. VBNC pathogens pose a significant risk for human and animal health as they can “wake up” back into a vegetative and virulent state. Although hundreds of bacterial species have been reported to enter a VBNC state in response to various stresses (e.g. thermal, osmotic, starvation, antibiotics), the molecular mechanisms governing this phenotypic switch remains largely elusive. Here, we report an in-depth characterization of the VBNC state transition process in the bacterial pathogen Listeria monocytogenes in response to nutritional deprivation. We found that starvation in mineral water drives L.monocytogenes into a VBNC state via a unique mechanism of cell wall shedding that generates cellwall-deficient coccoid forms. Transcriptomic and gene-targeted approaches revealed the stress response regulator SigB and the autolysin NamA as major mediators of cell wall loss and VBNC state transition. Overall design: Comparison between cells in the first day and after 7 days of incubation in mineral water, with biological triplicates.pathogen:bacteriumpathogenxref:PubMed:39358320ENAbacteriafalseDeep starvation induces loss of cell wall and dormancy in ListeriaDeep starvation induces loss of cell wall and dormancy in Listeria2025-09-242024-08-03PRJNA1031675GSE24615716996339358320