ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/049/SRR26631549/SRR26631549_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/043/SRR26631543/SRR26631543_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/045/SRR26631545/SRR26631545_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/047/SRR26631547/SRR26631547_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/045/SRR26631545/SRR26631545_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/048/SRR26631548/SRR26631548_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/048/SRR26631548/SRR26631548_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/046/SRR26631546/SRR26631546_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/042/SRR26631542/SRR26631542_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/042/SRR26631542/SRR26631542_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/044/SRR26631544/SRR26631544_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/049/SRR26631549/SRR26631549_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/047/SRR26631547/SRR26631547_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/046/SRR26631546/SRR26631546_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/044/SRR26631544/SRR26631544_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR266/043/SRR26631543/SRR26631543_1.fastq.gzprimary200OKGenomics中山眼科中心https://www.ebi.ac.uk/ena/browser/view/PRJNA1034631Mus musculusTo explore the functions for Phb2 gene in early retinal degeneration, we performed RNA-seq analysis on the bulk retinas from control and photoreceptor-specific knockout Phb2 mice at 6 weeks of age. Compared with control mice, there were 778 differentially expressed genes (log2 |fold change|> 1, log10 adjusted p-values 1, log10 adjusted p-values < 0.05) identified to be associated with distinct biological processes. 585 genes were significantly upregulated and 192 genes were significantly downregulated in photoreceptor cell-specific knockout Phb2 mice. The KEGG pathways analysis of up-regulated and down-regulated genes and ranking the enrichment pathways according to the corrected p-value. Overall design: retina were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.RNA libraries were prepared for sequencing using standard Illumina protocolsENAfalseEffect of Phb2 depletion in retinal photoreceptors on gene expression in the bulk retina of C57BL/6J miceEffect of Phb2 depletion in retinal photoreceptors on gene expression in the bulk retina of C57BL/6J mice2025-09-242025-01-03PRJNA1034631GSE24680210090